ID   PUTP_STAEQ              Reviewed;         511 AA.
AC   Q5HN32;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Sodium/proline symporter;
DE   AltName: Full=Proline permease;
GN   Name=putP; OrderedLocusNames=SERP1440;
OS   Staphylococcus epidermidis (strain ATCC 35984 / RP62A).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176279;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35984 / RP62A;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
CC   -!- FUNCTION: Catalyzes the sodium-dependent uptake of extracellular L-
CC       proline. {ECO:0000250|UniProtKB:P07117}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out);
CC         Xref=Rhea:RHEA:28967, ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
CC         Evidence={ECO:0000250|UniProtKB:P07117};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC       family. {ECO:0000305}.
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DR   EMBL; CP000029; AAW54820.1; -; Genomic_DNA.
DR   RefSeq; WP_001830425.1; NC_002976.3.
DR   AlphaFoldDB; Q5HN32; -.
DR   SMR; Q5HN32; -.
DR   STRING; 176279.SERP1440; -.
DR   EnsemblBacteria; AAW54820; AAW54820; SERP1440.
DR   KEGG; ser:SERP1440; -.
DR   eggNOG; COG0591; Bacteria.
DR   HOGENOM; CLU_018808_15_2_9; -.
DR   OMA; CWTDLIQ; -.
DR   OrthoDB; 231452at2; -.
DR   Proteomes; UP000000531; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005298; F:proline:sodium symporter activity; IEA:InterPro.
DR   GO; GO:0031402; F:sodium ion binding; IEA:InterPro.
DR   GO; GO:0015824; P:proline transport; IEA:InterPro.
DR   CDD; cd11475; SLC5sbd_PutP; 1.
DR   Gene3D; 1.20.1730.10; -; 1.
DR   InterPro; IPR038377; Na/Glc_symporter_sf.
DR   InterPro; IPR011851; Na/Pro_symporter.
DR   InterPro; IPR001734; Na/solute_symporter.
DR   Pfam; PF00474; SSF; 1.
DR   TIGRFAMs; TIGR02121; Na_Pro_sym; 1.
DR   TIGRFAMs; TIGR00813; sss; 1.
DR   PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Ion transport; Membrane;
KW   Reference proteome; Sodium; Sodium transport; Symport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..511
FT                   /note="Sodium/proline symporter"
FT                   /id="PRO_0000364107"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        407..427
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        438..458
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        467..487
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   511 AA;  56039 MW;  148C74F0FC095D25 CRC64;
     MFTLGTALSN QIDANWQTYV MIIVYFIILL IIGFYGYRQA TGNLSEFMLG GRSIGPYITA
     LSAGASDMSG WMIMGLPGSV YSTGLSAIWI TIGLTLGAYI NYFVVAPRLR VYTEIAGDAI
     TLPDFFKNRL DDKKNIIKII SGLIIVVFFT LYTHSGFVSG GKLFESAFGL NYHAGLLIVA
     IIVIFYTFFG GYLAVSITDF FQGVIMLIAM VMVPIVALLK LNGWDTFHDI AQMKPTNLDL
     FRGTTVLGIV SLFSWGLGYF GQPHIIVRFM SIKSHKLLPK ARRLGISWMA VGLLGAIGVG
     LTGISFISER HIKLEDPETL FIVMSQILFH PLVGGFLLAA ILAAIMSTIS SQLLVTSSSL
     TEDFYKLIRG SDKASSHQKE FVLIGRLSVL LVAIVAITIA WHPNDTILNL VGNAWAGFGA
     AFSPLVLYSL YWKDLTRAGA ISGMVAGAVV VIVWISWIKP LATINAFFGM YEIIPGFIVS
     VLITYIVSKL TKKPDDYVIE NLNKVKHVVK E