ID   PUTP_STAES              Reviewed;         511 AA.
AC   Q8CNP2;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Sodium/proline symporter;
DE   AltName: Full=Proline permease;
GN   Name=putP; OrderedLocusNames=SE_1587;
OS   Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=176280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 12228 / FDA PCI 1200;
RX   PubMed=12950922; DOI=10.1046/j.1365-2958.2003.03671.x;
RA   Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q.,
RA   Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H.,
RA   Zhao G.-P., Qu D., Danchin A., Wen Y.-M.;
RT   "Genome-based analysis of virulence genes in a non-biofilm-forming
RT   Staphylococcus epidermidis strain (ATCC 12228).";
RL   Mol. Microbiol. 49:1577-1593(2003).
CC   -!- FUNCTION: Catalyzes the sodium-dependent uptake of extracellular L-
CC       proline. {ECO:0000250|UniProtKB:P07117}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out);
CC         Xref=Rhea:RHEA:28967, ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
CC         Evidence={ECO:0000250|UniProtKB:P07117};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC       family. {ECO:0000305}.
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DR   EMBL; AE015929; AAO05186.1; -; Genomic_DNA.
DR   RefSeq; NP_765142.1; NC_004461.1.
DR   RefSeq; WP_002484895.1; NZ_WBME01000010.1.
DR   AlphaFoldDB; Q8CNP2; -.
DR   SMR; Q8CNP2; -.
DR   STRING; 176280.SE_1587; -.
DR   EnsemblBacteria; AAO05186; AAO05186; SE_1587.
DR   KEGG; sep:SE_1587; -.
DR   PATRIC; fig|176280.10.peg.1551; -.
DR   eggNOG; COG0591; Bacteria.
DR   HOGENOM; CLU_018808_15_2_9; -.
DR   OMA; CWTDLIQ; -.
DR   Proteomes; UP000001411; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005298; F:proline:sodium symporter activity; IEA:InterPro.
DR   GO; GO:0031402; F:sodium ion binding; IEA:InterPro.
DR   GO; GO:0015824; P:proline transport; IEA:InterPro.
DR   CDD; cd11475; SLC5sbd_PutP; 1.
DR   Gene3D; 1.20.1730.10; -; 1.
DR   InterPro; IPR038377; Na/Glc_symporter_sf.
DR   InterPro; IPR011851; Na/Pro_symporter.
DR   InterPro; IPR001734; Na/solute_symporter.
DR   Pfam; PF00474; SSF; 1.
DR   TIGRFAMs; TIGR02121; Na_Pro_sym; 1.
DR   TIGRFAMs; TIGR00813; sss; 1.
DR   PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Ion transport; Membrane; Sodium;
KW   Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..511
FT                   /note="Sodium/proline symporter"
FT                   /id="PRO_0000364106"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        407..427
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        438..458
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        467..487
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   511 AA;  56025 MW;  2028D63BA046A568 CRC64;
     MFTLGTALSN QIDANWQTYV MIIVYFIILL IIGFYGYRQA TGNLSEFMLG GRSIGPYITA
     LSAGASDMSG WMIMGLPGSV YSTGLSAIWI TIGLTLGAYI NYFVVAPRLR VFTEIAGDAI
     TLPDFFKNRL DDKKNIIKII SGLIIVVFFT LYTHSGFVSG GKLFESAFGL NYHAGLLIVA
     IIVIFYTFFG GYLAVSITDF FQGVIMLIAM VMVPIVALLK LNGWDTFHDI AQMKPTNLDL
     FRGTTVLGIV SLFSWGLGYF GQPHIIVRFM SIKSHKLLPK ARRLGISWMA VGLLGAIGVG
     LTGISFISER HIKSEDPETL FIVMSQILFH PLVGGFLLAA ILAAIMSTIS SQLLVTSSSL
     TEDFYKLIRG SDKASSHQKE FVLIGRLSVL LVAIVAITIA WHPNDTILNL VGNAWAGFGA
     AFSPLVLYSL YWKDLTRAGA ISGMVAGAVV VIVWISWIKP LATINAFFGM YEIIPGFIIS
     VLITYIVSKL TKKPDDYVIE NLNKVKHIVK E