ID   PUTP_STAHJ              Reviewed;         512 AA.
AC   Q4L7L6;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Sodium/proline symporter;
DE   AltName: Full=Proline permease;
GN   Name=putP; OrderedLocusNames=SH1050;
OS   Staphylococcus haemolyticus (strain JCSC1435).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=279808;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCSC1435;
RX   PubMed=16237012; DOI=10.1128/jb.187.21.7292-7308.2005;
RA   Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y.,
RA   Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C.,
RA   Hiramatsu K.;
RT   "Whole-genome sequencing of Staphylococcus haemolyticus uncovers the
RT   extreme plasticity of its genome and the evolution of human-colonizing
RT   staphylococcal species.";
RL   J. Bacteriol. 187:7292-7308(2005).
CC   -!- FUNCTION: Catalyzes the sodium-dependent uptake of extracellular L-
CC       proline. {ECO:0000250|UniProtKB:P07117}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out);
CC         Xref=Rhea:RHEA:28967, ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
CC         Evidence={ECO:0000250|UniProtKB:P07117};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE04359.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AP006716; BAE04359.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_016931028.1; NC_007168.1.
DR   AlphaFoldDB; Q4L7L6; -.
DR   SMR; Q4L7L6; -.
DR   STRING; 279808.SH1050; -.
DR   PRIDE; Q4L7L6; -.
DR   EnsemblBacteria; BAE04359; BAE04359; SH1050.
DR   GeneID; 58062774; -.
DR   KEGG; sha:SH1050; -.
DR   eggNOG; COG0591; Bacteria.
DR   HOGENOM; CLU_018808_15_2_9; -.
DR   OrthoDB; 231452at2; -.
DR   Proteomes; UP000000543; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005298; F:proline:sodium symporter activity; IEA:InterPro.
DR   GO; GO:0031402; F:sodium ion binding; IEA:InterPro.
DR   GO; GO:0015824; P:proline transport; IEA:InterPro.
DR   CDD; cd11475; SLC5sbd_PutP; 1.
DR   Gene3D; 1.20.1730.10; -; 1.
DR   InterPro; IPR038377; Na/Glc_symporter_sf.
DR   InterPro; IPR011851; Na/Pro_symporter.
DR   InterPro; IPR001734; Na/solute_symporter.
DR   InterPro; IPR018212; Na/solute_symporter_CS.
DR   Pfam; PF00474; SSF; 1.
DR   TIGRFAMs; TIGR02121; Na_Pro_sym; 1.
DR   TIGRFAMs; TIGR00813; sss; 1.
DR   PROSITE; PS00457; NA_SOLUT_SYMP_2; 1.
DR   PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Ion transport; Membrane; Sodium;
KW   Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..512
FT                   /note="Sodium/proline symporter"
FT                   /id="PRO_0000364108"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        288..308
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        410..430
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        438..458
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        467..487
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   512 AA;  56122 MW;  AA5D80CABFC7CBFE CRC64;
     MLILGASLPS QVNTNWQTYI MIAIYFIILL IIGYYGYKQA TGNLSEYMLG GRSIGPYITA
     LSAGASDMSG WMIMGLPGSV YSTGLSAIWI TIGLTLGAYV NYLVVAPRLR VYTEVAGDAI
     TLPDFFKNRL NDKENIIKII SGLIIVVFFT LYTHSGFVSG GKLFESAFGL NYHFGLILVA
     VIVIAYTFFG GYLAVSITDF FQGVIMLIAM VMVPIVAMLQ LNGWDTFSRV AEMKPTNMDL
     FRGTTFLGIV SLFAWGLGYF GQPHIIVRFM SIKSQKMLPK ARRLGISWMT VGLLGAVGVG
     LTGIAFIPDN HIKMDDPETL FIIMSQILFH PLVGGFLLAA ILAAIMSTIS SQLLVTSSSL
     TEDFYKLIRG EERVKTHQKE FVLVGRLSVL VVAIVAIAIA WSPNDTILNL VGNAWAGFGA
     SFSPLVLFSL YWKKLTRAGA ISGMVAGALV VIIWIAWIKP LASINEIFGM YEIIPGFIIS
     VIVTYVVSLL TQHPGDFVER DIEKVKSIVR EK