PUUR_ECOLI
ID PUUR_ECOLI Reviewed; 185 AA.
AC P0A9U6; P38522; P76839; P77417;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=HTH-type transcriptional regulator PuuR;
GN Name=puuR; Synonyms=ycjC; OrderedLocusNames=b1299, JW1292;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 65-185.
RX PubMed=1840553; DOI=10.1016/0378-1119(91)90028-a;
RA Heim R., Strehler E.E.;
RT "Cloning an Escherichia coli gene encoding a protein remarkably similar to
RT mammalian aldehyde dehydrogenases.";
RL Gene 99:15-23(1991).
RN [5]
RP FUNCTION AS A REPRESSOR, AND NOMENCLATURE.
RC STRAIN=K12;
RX PubMed=15590624; DOI=10.1074/jbc.m411114200;
RA Kurihara S., Oda S., Kato K., Kim H.G., Koyanagi T., Kumagai H., Suzuki H.;
RT "A novel putrescine utilization pathway involves gamma-glutamylated
RT intermediates of Escherichia coli K-12.";
RL J. Biol. Chem. 280:4602-4608(2005).
RN [6]
RP FUNCTION AS HTH-TYPE TRANSCRIPTIONAL REGULATOR, AND DISRUPTION PHENOTYPE.
RX PubMed=20639325; DOI=10.1128/jb.00308-10;
RA Kurihara S., Kato K., Asada K., Kumagai H., Suzuki H.;
RT "A putrescine-inducible pathway comprising PuuE-YneI in which gamma-
RT aminobutyrate is degraded into succinate in Escherichia coli K-12.";
RL J. Bacteriol. 192:4582-4591(2010).
CC -!- FUNCTION: Represses puuA, puuD and puuP. {ECO:0000269|PubMed:15590624,
CC ECO:0000269|PubMed:20639325}.
CC -!- PATHWAY: Amine and polyamine degradation; putrescine degradation
CC [regulation].
CC -!- DISRUPTION PHENOTYPE: Cells enhance strongly the transcription of puuE.
CC {ECO:0000269|PubMed:20639325}.
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DR EMBL; U00096; AAC74381.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA14868.1; -; Genomic_DNA.
DR EMBL; M38433; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; F64878; F64878.
DR RefSeq; NP_415815.1; NC_000913.3.
DR RefSeq; WP_001278727.1; NZ_STEB01000005.1.
DR AlphaFoldDB; P0A9U6; -.
DR SMR; P0A9U6; -.
DR BioGRID; 4263529; 9.
DR DIP; DIP-48257N; -.
DR IntAct; P0A9U6; 1.
DR STRING; 511145.b1299; -.
DR jPOST; P0A9U6; -.
DR PaxDb; P0A9U6; -.
DR PRIDE; P0A9U6; -.
DR EnsemblBacteria; AAC74381; AAC74381; b1299.
DR EnsemblBacteria; BAA14868; BAA14868; BAA14868.
DR GeneID; 67417375; -.
DR GeneID; 945886; -.
DR KEGG; ecj:JW1292; -.
DR KEGG; eco:b1299; -.
DR PATRIC; fig|511145.12.peg.1355; -.
DR EchoBASE; EB2327; -.
DR eggNOG; COG1396; Bacteria.
DR eggNOG; COG1917; Bacteria.
DR HOGENOM; CLU_085376_1_4_6; -.
DR InParanoid; P0A9U6; -.
DR OMA; VELLFVH; -.
DR PhylomeDB; P0A9U6; -.
DR BioCyc; EcoCyc:EG12431-MON; -.
DR UniPathway; UPA00188; -.
DR PRO; PR:P0A9U6; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0009447; P:putrescine catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:EcoCyc.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR013096; Cupin_2.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF07883; Cupin_2; 1.
DR Pfam; PF01381; HTH_3; 1.
DR SMART; SM00530; HTH_XRE; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
DR PROSITE; PS50943; HTH_CROC1; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..185
FT /note="HTH-type transcriptional regulator PuuR"
FT /id="PRO_0000149731"
FT DOMAIN 12..66
FT /note="HTH cro/C1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT DNA_BIND 23..42
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00257"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 185 AA; 20092 MW; D7D0D3FD794A7768 CRC64;
MSDEGLAPGK RLSEIRQQQG LSQRRAAELS GLTHSAISTI EQDKVSPAIS TLQKLLKVYG
LSLSEFFSEP EKPDEPQVVI NQDDLIEMGS QGVSMKLVHN GNPNRTLAMI FETYQPGTTT
GERIKHQGEE IGTVLEGEIV LTINGQDYHL VAGQSYAINT GIPHSFSNTS AGICRIISAH
TPTTF
