PVCA_PSEAE
ID PVCA_PSEAE Reviewed; 328 AA.
AC Q9I1L5;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=L-tyrosine isonitrile synthase {ECO:0000305};
DE EC=4.1.99.24 {ECO:0000305|PubMed:18824174};
DE AltName: Full=Paerucumarin biosynthesis protein PvcA {ECO:0000305};
GN Name=pvcA {ECO:0000303|PubMed:18689486};
GN OrderedLocusNames=PA2254 {ECO:0000312|EMBL:AAG05642.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18689486; DOI=10.1128/jb.00801-08;
RA Clarke-Pearson M.F., Brady S.F.;
RT "Paerucumarin, a new metabolite produced by the pvc gene cluster from
RT Pseudomonas aeruginosa.";
RL J. Bacteriol. 190:6927-6930(2008).
RN [3] {ECO:0007744|PDB:3E59}
RP X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS), FUNCTION, SUBUNIT, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=18824174; DOI=10.1016/j.jmb.2008.09.027;
RA Drake E.J., Gulick A.M.;
RT "Three-dimensional structures of Pseudomonas aeruginosa PvcA and PvcB, two
RT proteins involved in the synthesis of 2-isocyano-6,7-dihydroxycoumarin.";
RL J. Mol. Biol. 384:193-205(2008).
CC -!- FUNCTION: Involved in the biosynthesis of paerucumarin, a cyclized
CC isocyano derivative of tyrosine (PubMed:18689486, PubMed:18824174).
CC Responsible for the synthesis of the isonitrile group on tyrosine using
CC the C2 of ribulose 5-phosphate as the source of the carbon atom
CC (Probable). {ECO:0000269|PubMed:18689486, ECO:0000269|PubMed:18824174,
CC ECO:0000305|PubMed:18824174}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-ribulose 5-phosphate + L-tyrosine = (2S)-3-(4-
CC hydroxyphenyl)-2-isocyanopropanoate + formaldehyde + H(+) + H2O +
CC hydroxyacetone + phosphate; Xref=Rhea:RHEA:45732, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16842, ChEBI:CHEBI:27957,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58121, ChEBI:CHEBI:58315,
CC ChEBI:CHEBI:140647; EC=4.1.99.24;
CC Evidence={ECO:0000305|PubMed:18824174};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45733;
CC Evidence={ECO:0000305|PubMed:18824174};
CC -!- SUBUNIT: Monomer in solution. {ECO:0000269|PubMed:18824174}.
CC -!- DISRUPTION PHENOTYPE: Disruption of the gene maintains the ability of
CC the strain to produce the pyoverdine siderophore (PubMed:18689486,
CC PubMed:18824174). Disruption mutant cannot produce paerucumarin
CC (PubMed:18689486). {ECO:0000269|PubMed:18689486,
CC ECO:0000269|PubMed:18824174}.
CC -!- SIMILARITY: Belongs to the isocyanide synthase family. {ECO:0000305}.
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DR EMBL; AE004091; AAG05642.1; -; Genomic_DNA.
DR PIR; H83363; H83363.
DR RefSeq; NP_250944.1; NC_002516.2.
DR RefSeq; WP_003113733.1; NZ_QZGE01000014.1.
DR PDB; 3E59; X-ray; 2.10 A; A/B/C/D=1-328.
DR PDBsum; 3E59; -.
DR AlphaFoldDB; Q9I1L5; -.
DR SMR; Q9I1L5; -.
DR STRING; 287.DR97_6296; -.
DR PaxDb; Q9I1L5; -.
DR PRIDE; Q9I1L5; -.
DR DNASU; 878099; -.
DR EnsemblBacteria; AAG05642; AAG05642; PA2254.
DR GeneID; 878099; -.
DR KEGG; pae:PA2254; -.
DR PATRIC; fig|208964.12.peg.2356; -.
DR PseudoCAP; PA2254; -.
DR HOGENOM; CLU_038280_0_0_6; -.
DR InParanoid; Q9I1L5; -.
DR OMA; TRDDWLT; -.
DR BioCyc; MetaCyc:MON-20393; -.
DR BioCyc; PAER208964:G1FZ6-2293-MON; -.
DR BRENDA; 4.1.99.24; 5087.
DR EvolutionaryTrace; Q9I1L5; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR InterPro; IPR007817; Isocyanide_synthase_DIT1.
DR InterPro; IPR017133; PvcA.
DR PANTHER; PTHR37285; PTHR37285; 1.
DR Pfam; PF05141; DIT1_PvcA; 1.
DR PIRSF; PIRSF037196; Pyoverdine_chromoph_PvcA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Lyase; Reference proteome.
FT CHAIN 1..328
FT /note="L-tyrosine isonitrile synthase"
FT /id="PRO_0000453966"
FT HELIX 9..18
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 25..28
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 33..53
FT /evidence="ECO:0007829|PDB:3E59"
FT STRAND 58..62
FT /evidence="ECO:0007829|PDB:3E59"
FT TURN 72..74
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 82..101
FT /evidence="ECO:0007829|PDB:3E59"
FT STRAND 106..110
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 114..117
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 118..120
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 125..142
FT /evidence="ECO:0007829|PDB:3E59"
FT STRAND 145..149
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 152..154
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 156..161
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 165..176
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 180..187
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 191..208
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 217..245
FT /evidence="ECO:0007829|PDB:3E59"
FT STRAND 246..255
FT /evidence="ECO:0007829|PDB:3E59"
FT STRAND 261..266
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 278..280
FT /evidence="ECO:0007829|PDB:3E59"
FT STRAND 281..293
FT /evidence="ECO:0007829|PDB:3E59"
FT HELIX 295..300
FT /evidence="ECO:0007829|PDB:3E59"
FT STRAND 304..308
FT /evidence="ECO:0007829|PDB:3E59"
FT STRAND 311..317
FT /evidence="ECO:0007829|PDB:3E59"
SQ SEQUENCE 328 AA; 37134 MW; DE646CA9BEF43B29 CRC64;
MYAIAEDTLP ARVLKELLLY RRRYPEHRQS ASEADEIRRI EQVQLPRIAA FIEAGEPIEF
VLPAFPAKSP NPGKVLDSRP DMAERLSLSF LNHLCQRIQL FYAPGAKITV CSDGRVFGDL
VRIGDAHISA YQDALRLMIE EIGATHIGVF NLEDVRAFEA QRDNHEQLRQ LLIGGYAEPL
ESIRETLLAS EEGLLLYRAI TRFLYEDGLT PDYQGSKTAL QRDAKERAYG VIQRSWAWGA
LLADQFPRAI RLSIHPQPAD SLKFGIHMMP TRDDWLTPWH GVAVNTEDRF VLMKRSEVLE
LGGELVQING QPSHYRLPAR AARRAAVA
