ID   PVHB_TALVA              Reviewed;         371 AA.
AC   A0A3Q9U4G3;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   10-APR-2019, sequence version 1.
DT   03-AUG-2022, entry version 6.
DE   RecName: Full=Diels-Alderase pvhB {ECO:0000303|PubMed:30609896};
DE            EC=5.5.1.- {ECO:0000269|PubMed:30609896};
DE   AltName: Full=Varicidin biosynthesis cluster protein B {ECO:0000303|PubMed:30609896};
GN   Name=pvhB {ECO:0000303|PubMed:30609896};
OS   Talaromyces variabilis (Penicillium variabile).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae; Talaromyces.
OX   NCBI_TaxID=28576;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   PATHWAY.
RC   STRAIN=HXQ-H-1;
RX   PubMed=30609896; DOI=10.1021/jacs.8b12010;
RA   Tan D., Jamieson C.S., Ohashi M., Tang M.C., Houk K.N., Tang Y.;
RT   "Genome-mined Diels-Alderase catalyzes formation of the cis-
RT   octahydrodecalins of varicidin A and B.";
RL   J. Am. Chem. Soc. 141:769-773(2019).
CC   -!- FUNCTION: Diels-Alderase; part of the gene cluster that mediates the
CC       biosynthesis of varicidin A, an antifungal natural product containing a
CC       cis-octahydrodecalin core (PubMed:30609896). The PKS module of pvhA
CC       together with the enoylreductase pvhC catalyze the formation of the
CC       polyketide unit which is then conjugated to L-isoleucine by the
CC       condensation domain of the NRPS module (PubMed:30609896). Activity of
CC       the Dieckmann cyclase domain (RED) of pvhA results in release of an
CC       acyclic tetramate (PubMed:30609896). The cytochrome P450 monooxygenase
CC       pvhE then catalyzes the oxidation of the C21 methyl group to a to
CC       carboxylate group (PubMed:30609896). The methyltransferase pvhD then
CC       further methylates the pvhE product (PubMed:30609896). The Diels-
CC       Alderase pvhB is able to catalyzes Diels-Alder cycloaddition using both
CC       pvhE and pvhD products as substrates to form the decalin ring, yielding
CC       varicidin B and A, respectively (PubMed:30609896).
CC       {ECO:0000269|PubMed:30609896}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:30609896}.
CC   -!- SIMILARITY: Belongs to the Diels-Alderase family. {ECO:0000305}.
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DR   EMBL; MK359105; AZZ09608.1; -; Genomic_DNA.
DR   EMBL; MK376933; AZZ09615.1; -; Genomic_DNA.
DR   SMR; A0A3Q9U4G3; -.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Isomerase.
FT   CHAIN           1..371
FT                   /note="Diels-Alderase pvhB"
FT                   /id="PRO_0000453341"
SQ   SEQUENCE   371 AA;  40854 MW;  B004FEA3124EA2A8 CRC64;
     MGNKMTKRIS HFNIENAIIP GEVSASAHVP GENNIFPKYS SNINDRTWEI WCCEGINHEG
     AGVMFGFQRA AFNKKMGGGW RVQIIAVWPD GKNWYKALAL PESIFEESMA SGDITSVWKT
     ADNSTNVSFT YHKVDNKIDV VVCIPGVATG KASFQGQPGD SGLSTSPSLG PYVSYMRPVG
     RADMTSDMIF HFPDDGTTRQ ISFSDGHGGF DRYWGEKLWL SIQSESYFMR AFLGPYVIQI
     IRLVSTEELG KQQWPIARLF HQGKLVCAPQ RCGSPDEQGI SQDTLVMSKV HDSDTGALSA
     EFHDKSTGYD LTFVEAGNAR RRWTFTVRHQ TKIWSLPIGP KGGNSGFVEH VCGGLAGETC
     EGLGVSGQIV Y