ID   AAEA_ECOSM              Reviewed;         310 AA.
AC   B1LGK7;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=p-hydroxybenzoic acid efflux pump subunit AaeA {ECO:0000255|HAMAP-Rule:MF_01544};
DE            Short=pHBA efflux pump protein A {ECO:0000255|HAMAP-Rule:MF_01544};
GN   Name=aaeA {ECO:0000255|HAMAP-Rule:MF_01544};
GN   OrderedLocusNames=EcSMS35_3537;
OS   Escherichia coli (strain SMS-3-5 / SECEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=439855;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SMS-3-5 / SECEC;
RX   PubMed=18708504; DOI=10.1128/jb.00661-08;
RA   Fricke W.F., Wright M.S., Lindell A.H., Harkins D.M., Baker-Austin C.,
RA   Ravel J., Stepanauskas R.;
RT   "Insights into the environmental resistance gene pool from the genome
RT   sequence of the multidrug-resistant environmental isolate Escherichia coli
RT   SMS-3-5.";
RL   J. Bacteriol. 190:6779-6794(2008).
CC   -!- FUNCTION: Forms an efflux pump with AaeB. {ECO:0000255|HAMAP-
CC       Rule:MF_01544}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01544}; Single-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01544}.
CC   -!- INDUCTION: Positively coregulated with aaeB and aaeX by AaeR.
CC       {ECO:0000255|HAMAP-Rule:MF_01544}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000255|HAMAP-Rule:MF_01544}.
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DR   EMBL; CP000970; ACB18923.1; -; Genomic_DNA.
DR   RefSeq; WP_000854033.1; NC_010498.1.
DR   AlphaFoldDB; B1LGK7; -.
DR   SMR; B1LGK7; -.
DR   EnsemblBacteria; ACB18923; ACB18923; EcSMS35_3537.
DR   KEGG; ecm:EcSMS35_3537; -.
DR   HOGENOM; CLU_018816_15_2_6; -.
DR   OMA; EVEPTFN; -.
DR   Proteomes; UP000007011; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01544; AaeA; 1.
DR   InterPro; IPR039562; MFP_biotin_lipoyl_2.
DR   InterPro; IPR022871; PHBA_efflux_pump_AaeA.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF13533; Biotin_lipoyl_2; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..310
FT                   /note="p-hydroxybenzoic acid efflux pump subunit AaeA"
FT                   /id="PRO_1000146718"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01544"
SQ   SEQUENCE   310 AA;  34761 MW;  BA6F840F325B6F23 CRC64;
     MKTLIRKFSR TAITVVLVIL AFIAIFNAWV YYTESPWTRD ARFSADVVAI APDVSGLITQ
     VNVHDNQLVK KGQVLFTIDQ PRYQKALEEA QADVAYYQVL AQEKRQEAGR RNRLGVQAMS
     REEIDQANNV LQTVLHQLAK AQATRDLAKL DLERTVIRAP ADGWVTNLNV YTGEFITRGS
     TAVALVKQNS FYVLAYMEET KLEGVRPGYR AEITPLGSNK VLKGTVDSVA AGVTNASSTR
     DDKGMATIDS NLEWVRLAQR VPVRIRLDNQ QENIWPAGTT ATVVVTGKQD RDESQDSFFR
     KMAHRLREFG