PWP1_MOUSE
ID PWP1_MOUSE Reviewed; 501 AA.
AC Q99LL5; Q9D6T6;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Periodic tryptophan protein 1 homolog;
GN Name=Pwp1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Tongue;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II, and FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic brain;
RX PubMed=15345747; DOI=10.1074/mcp.m400085-mcp200;
RA Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
RT "Phosphoproteomic analysis of the developing mouse brain.";
RL Mol. Cell. Proteomics 3:1093-1101(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49; SER-58 AND SER-493, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Chromatin-associated factor that regulates transcription (By
CC similarity). Regulates Pol I-mediated rRNA biogenesis and, probably,
CC Pol III-mediated transcription (By similarity). Regulates the
CC epigenetic status of rDNA (By similarity).
CC {ECO:0000250|UniProtKB:Q13610}.
CC -!- SUBUNIT: Associates with the RNA polymerase (Pol I) complex. Interacts
CC with POLR1E. {ECO:0000250|UniProtKB:Q13610}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q13610}. Nucleus,
CC nucleolus {ECO:0000250|UniProtKB:Q13610}. Chromosome
CC {ECO:0000250|UniProtKB:Q13610}. Note=Associates with chromatin regions
CC of rDNA. {ECO:0000250|UniProtKB:Q13610}.
CC -!- SIMILARITY: Belongs to the WD repeat PWP1 family. {ECO:0000305}.
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DR EMBL; AK009972; BAB26622.1; -; mRNA.
DR EMBL; AK082158; BAC38427.1; -; mRNA.
DR EMBL; BC003199; AAH03199.1; -; mRNA.
DR EMBL; BC023137; AAH23137.1; -; mRNA.
DR CCDS; CCDS24092.1; -.
DR RefSeq; NP_598754.2; NM_133993.3.
DR AlphaFoldDB; Q99LL5; -.
DR SMR; Q99LL5; -.
DR BioGRID; 222026; 27.
DR IntAct; Q99LL5; 4.
DR STRING; 10090.ENSMUSP00000001836; -.
DR iPTMnet; Q99LL5; -.
DR PhosphoSitePlus; Q99LL5; -.
DR EPD; Q99LL5; -.
DR jPOST; Q99LL5; -.
DR MaxQB; Q99LL5; -.
DR PaxDb; Q99LL5; -.
DR PeptideAtlas; Q99LL5; -.
DR PRIDE; Q99LL5; -.
DR ProteomicsDB; 302036; -.
DR Antibodypedia; 18254; 87 antibodies from 25 providers.
DR DNASU; 103136; -.
DR Ensembl; ENSMUST00000001836; ENSMUSP00000001836; ENSMUSG00000001785.
DR GeneID; 103136; -.
DR KEGG; mmu:103136; -.
DR UCSC; uc007gnc.2; mouse.
DR CTD; 11137; -.
DR MGI; MGI:1914735; Pwp1.
DR VEuPathDB; HostDB:ENSMUSG00000001785; -.
DR eggNOG; KOG0270; Eukaryota.
DR GeneTree; ENSGT00390000017324; -.
DR HOGENOM; CLU_023867_1_1_1; -.
DR InParanoid; Q99LL5; -.
DR OMA; CFVPRGV; -.
DR OrthoDB; 737539at2759; -.
DR PhylomeDB; Q99LL5; -.
DR TreeFam; TF314868; -.
DR BioGRID-ORCS; 103136; 27 hits in 72 CRISPR screens.
DR ChiTaRS; Pwp1; mouse.
DR PRO; PR:Q99LL5; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q99LL5; protein.
DR Bgee; ENSMUSG00000001785; Expressed in primitive streak and 258 other tissues.
DR ExpressionAtlas; Q99LL5; baseline and differential.
DR Genevisible; Q99LL5; MM.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:1990889; F:H4K20me3 modified histone binding; IDA:MGI.
DR GO; GO:0034773; P:histone H4-K20 trimethylation; IMP:MGI.
DR GO; GO:0033140; P:negative regulation of peptidyl-serine phosphorylation of STAT protein; IMP:MGI.
DR GO; GO:2000738; P:positive regulation of stem cell differentiation; IMP:MGI.
DR GO; GO:1901838; P:positive regulation of transcription of nucleolar large rRNA by RNA polymerase I; ISO:MGI.
DR GO; GO:0006364; P:rRNA processing; IEA:InterPro.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR044285; PWP1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR14091; PTHR14091; 1.
DR Pfam; PF00400; WD40; 3.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Chromosome; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW Ribosome biogenesis; Transcription; Transcription regulation; WD repeat.
FT CHAIN 1..501
FT /note="Periodic tryptophan protein 1 homolog"
FT /id="PRO_0000051172"
FT REPEAT 185..229
FT /note="WD 1"
FT REPEAT 253..293
FT /note="WD 2"
FT REPEAT 296..336
FT /note="WD 3"
FT REPEAT 382..422
FT /note="WD 4"
FT REPEAT 427..467
FT /note="WD 5"
FT REGION 38..107
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..107
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 21
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q13610"
FT MOD_RES 49
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:15345747,
FT ECO:0007744|PubMed:17242355, ECO:0007744|PubMed:19144319,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 58
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 85
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q13610"
FT MOD_RES 484
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q13610"
FT MOD_RES 493
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 247
FT /note="K -> R (in Ref. 1; BAB26622)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 501 AA; 55587 MW; 704A050633F0C2B1 CRC64;
MNRSRQVTCV AWVRRGVAKE TPDKVELSKE EVNRLIAEAK GKLQEEGGSE EEEAGNPSED
GMQSGPTQAP PRESLEDGDP QDDRTLDDDE LAGYDLDNYD EEDNPDAETI GESLLGLTVY
GSNDQDPYVT LKDTEQYEHE DFLIKPTDNL IVCGRAEQEQ CNLEVHVYNQ EEESFYVHHD
ILLSAYPLSV EWLNFDPSPD ASTGNYIAVG NMTPVIEVWD LDIVDSLEPV FTLGSKLSKK
KKKKGKKSSS AEGHTDAVLD LSWNKTVRNV LASASADSTV VLWDLSVGKS VARLTAHTDK
VQTLQFHPFE AQTLISGSYD KSVALYDCRD PSQNHRQWRF SGQIERVTWN HFSPCHFLAS
TDDGFVYNLD ARSDKPIFTL NAHNDEISGL DLSSQIKGCL VTASADKFVK IWDILGDRPS
LIHSRDMKMG VLFCSSCCPD LPFVYAFGGQ KEGLRVWDIS TVSSVNEAFG RRERLVIGGT
KGLSVSGPCG SRSPQQTPME S
