PWP2A_XENTR
ID PWP2A_XENTR Reviewed; 784 AA.
AC F7AQ22; B1H3K5;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2017, sequence version 2.
DT 03-AUG-2022, entry version 42.
DE RecName: Full=PWWP domain-containing protein 2A;
GN Name=pwwp2a;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=20431018; DOI=10.1126/science.1183670;
RA Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT "The genome of the Western clawed frog Xenopus tropicalis.";
RL Science 328:633-636(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 115-784.
RC TISSUE=Testis;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=28645917; DOI=10.15252/embj.201695757;
RA Puenzeler S., Link S., Wagner G., Keilhauer E.C., Kronbeck N.,
RA Spitzer R.M., Leidescher S., Markaki Y., Mentele E., Regnard C.,
RA Schneider K., Takahashi D., Kusakabe M., Vardabasso C., Zink L.M.,
RA Straub T., Bernstein E., Harata M., Leonhardt H., Mann M., Rupp R.A.,
RA Hake S.B.;
RT "Multivalent binding of PWWP2A to H2A.Z regulates mitosis and neural crest
RT differentiation.";
RL EMBO J. 36:2263-2279(2017).
CC -!- FUNCTION: H2A.Z-specific chromatin binding protein which plays an
CC important role in the neural crest cell differentiation and/or
CC migration during early development and is essential for the development
CC of the head and eye (PubMed:28645917). Acts as an adapter between
CC distinct nucleosome components (H3K36me3 or H2A.Z) and chromatin-
CC modifying complexes, contributing to the regulation of the levels of
CC histone acetylation at actively transcribed genes (By similarity).
CC {ECO:0000250|UniProtKB:Q96N64, ECO:0000269|PubMed:28645917}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q96N64}.
CC -!- DEVELOPMENTAL STAGE: Expressed early during development in the
CC neuroectoderm and neural crest. {ECO:0000269|PubMed:28645917}.
CC -!- DISRUPTION PHENOTYPE: Embryos exhibit lack of retinal tissue combined
CC with a reduced head size. Defects in neural crest stem cell migration
CC and differentiation seen. {ECO:0000269|PubMed:28645917}.
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DR EMBL; AAMC01100632; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAMC01100633; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAMC01100634; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC161427; AAI61427.1; -; mRNA.
DR AlphaFoldDB; F7AQ22; -.
DR SMR; F7AQ22; -.
DR STRING; 8364.ENSXETP00000012601; -.
DR PaxDb; F7AQ22; -.
DR eggNOG; ENOG502QU6V; Eukaryota.
DR HOGENOM; CLU_020678_0_0_1; -.
DR InParanoid; F7AQ22; -.
DR TreeFam; TF331271; -.
DR Proteomes; UP000008143; Genome assembly.
DR Proteomes; UP000790000; Unplaced.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0035064; F:methylated histone binding; ISS:UniProtKB.
DR GO; GO:0001654; P:eye development; IMP:UniProtKB.
DR GO; GO:0060322; P:head development; IMP:UniProtKB.
DR GO; GO:1901675; P:negative regulation of histone H3-K27 acetylation; ISS:UniProtKB.
DR GO; GO:2000616; P:negative regulation of histone H3-K9 acetylation; ISS:UniProtKB.
DR GO; GO:0014033; P:neural crest cell differentiation; IMP:UniProtKB.
DR GO; GO:0001755; P:neural crest cell migration; IMP:UniProtKB.
DR InterPro; IPR000313; PWWP_dom.
DR Pfam; PF00855; PWWP; 1.
DR SMART; SM00293; PWWP; 1.
DR PROSITE; PS50812; PWWP; 1.
PE 2: Evidence at transcript level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..784
FT /note="PWWP domain-containing protein 2A"
FT /id="PRO_0000441730"
FT DOMAIN 684..744
FT /note="PWWP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00162"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 244..272
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 463..567
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 605..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 244..266
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 473..500
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 505..519
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 520..567
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 625..654
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 444
FT /note="E -> K (in Ref. 2; AAI61427)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 784 AA; 87069 MW; D76258ED4D6D6699 CRC64;
MAAVAGAPGP GEGGESEQDS ETIPGERRLG RLSCEPMEAE LDSERRSLVR CEPCCGMEID
TQENAGPDIE QLGDSGAEQV YGIHTNSEFV SVSAVDYMGI NQGQIAPENR KARSEQLPRE
PFLQRQPIPM ILSEVTDSGI YTGLCLEEAK FSSNNGSEAS LNYSECDNRS VGTTEGSVVT
SGIDSLIPGS EVQVTLDHII QDALVVSFCH GNRIFSGVLM DLSKRFGPHG IPVTVHPRRE
YKNKPVESIQ EESKSFHEEP LVKSEENSPE DVTPIQQLEN CKVQNLWTTK PPPLFHEGAP
YPPPLFIRDT YNQSIPQPPP RKIKRPKKKI YREEPTSIMN AIKLRPRQVL CDKCKNNVVA
DKKEIRKVAT DSYKTEEGKR RRHETVTTVN KKLKTDHKVN GKSQNESQKR TPITKVTNLA
HGRGKVVKVP SQTSAAKTQM HTKEVLQNKN MDHVKAREVL KMAKEKAQKK QKVTTSSKNA
HSKVHFTRRL QNSSSGTLPP RLRIKPQRYR NEENDSSLKP GLETLRSSKI GIKPQTRYSA
TRSAGETPSE IQSPSNGPEE ISSEMQDTNV CVPPEEQDLQ QTLGKRGSKS NITVYMTINQ
KKANSSSASV CSSDSTDDMK SSHSECSSTE NFDFPPGSMH APSSSSSSSA SSKEEKKLSN
SLKIKMFSKN VSKCVTPDGR TICVGDIVWA KIYGFPWWPA RILAITVSRK DTGLLVRQEA
RISWFGSPTT SFLALSQLTP FLENFQSRFN KKRKGLYRKA ITEAAKAAKQ LTPEVRALLT
QFET
