PWP2_MOUSE
ID PWP2_MOUSE Reviewed; 919 AA.
AC Q8BU03; Q8BTR0;
DT 09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Periodic tryptophan protein 2 homolog;
GN Name=Pwp2; Synonyms=Pwp2h;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-895, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-891; SER-895; SER-898 AND
RP SER-902, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-55, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat PWP2 family. {ECO:0000305}.
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DR EMBL; AK088254; BAC40239.1; -; mRNA.
DR EMBL; AK088983; BAC40686.1; -; mRNA.
DR CCDS; CCDS35958.1; -.
DR RefSeq; NP_083822.1; NM_029546.2.
DR AlphaFoldDB; Q8BU03; -.
DR SMR; Q8BU03; -.
DR BioGRID; 225929; 41.
DR STRING; 10090.ENSMUSP00000045812; -.
DR iPTMnet; Q8BU03; -.
DR PhosphoSitePlus; Q8BU03; -.
DR SwissPalm; Q8BU03; -.
DR EPD; Q8BU03; -.
DR jPOST; Q8BU03; -.
DR MaxQB; Q8BU03; -.
DR PaxDb; Q8BU03; -.
DR PRIDE; Q8BU03; -.
DR ProteomicsDB; 301927; -.
DR DNASU; 110816; -.
DR Ensembl; ENSMUST00000042556; ENSMUSP00000045812; ENSMUSG00000032834.
DR GeneID; 110816; -.
DR KEGG; mmu:110816; -.
DR UCSC; uc007fxk.1; mouse.
DR CTD; 5822; -.
DR MGI; MGI:1341200; Pwp2.
DR VEuPathDB; HostDB:ENSMUSG00000032834; -.
DR eggNOG; KOG0291; Eukaryota.
DR GeneTree; ENSGT00550000074981; -.
DR HOGENOM; CLU_010458_0_0_1; -.
DR InParanoid; Q8BU03; -.
DR OMA; QSEQYIM; -.
DR OrthoDB; 290630at2759; -.
DR PhylomeDB; Q8BU03; -.
DR TreeFam; TF300853; -.
DR Reactome; R-MMU-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR BioGRID-ORCS; 110816; 28 hits in 75 CRISPR screens.
DR ChiTaRS; Pwp2; mouse.
DR PRO; PR:Q8BU03; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q8BU03; protein.
DR Bgee; ENSMUSG00000032834; Expressed in primitive streak and 246 other tissues.
DR ExpressionAtlas; Q8BU03; baseline and differential.
DR Genevisible; Q8BU03; MM.
DR GO; GO:0034388; C:Pwp2p-containing subcomplex of 90S preribosome; IBA:GO_Central.
DR GO; GO:0032040; C:small-subunit processome; IBA:GO_Central.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR027145; PWP2.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR007148; SSU_processome_Utp12.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR PANTHER; PTHR19858; PTHR19858; 1.
DR Pfam; PF04003; Utp12; 1.
DR Pfam; PF00400; WD40; 3.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 13.
DR SUPFAM; SSF50998; SSF50998; 2.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Acetylation; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW WD repeat.
FT CHAIN 1..919
FT /note="Periodic tryptophan protein 2 homolog"
FT /id="PRO_0000051176"
FT REPEAT 12..50
FT /note="WD 1"
FT REPEAT 53..93
FT /note="WD 2"
FT REPEAT 94..132
FT /note="WD 3"
FT REPEAT 142..181
FT /note="WD 4"
FT REPEAT 186..225
FT /note="WD 5"
FT REPEAT 286..325
FT /note="WD 6"
FT REPEAT 328..368
FT /note="WD 7"
FT REPEAT 371..410
FT /note="WD 8"
FT REPEAT 413..452
FT /note="WD 9"
FT REPEAT 456..498
FT /note="WD 10"
FT REPEAT 499..538
FT /note="WD 11"
FT REPEAT 541..580
FT /note="WD 12"
FT REPEAT 603..642
FT /note="WD 13"
FT REPEAT 700..740
FT /note="WD 14"
FT REGION 238..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 885..919
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 900..919
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 55
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 891
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 895
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 898
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 902
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 202
FT /note="D -> H (in Ref. 1; BAC40686)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 919 AA; 102910 MW; 4B9BAC5B60A6A2D0 CRC64;
MKFAYRFSNL LGTVYRCGNL NFTHDGNSVI SPVGNRVTVF DLKNNRSNTL PLATKYNIKC
VGLSPDGRLA IIVDEGGAAL LVSLVCRSVL HHFHFKGSVH SVSFSPDGRK FVVTKGNIAQ
MYHAPGKKRE FNAFVLDKTY FGPYDETTCI DWTDDSKCFV VGSKDMSTWV FGAERWDNLI
YYALSGHKDA IVACFFESNS LDLYSLSQDG ALCVWQCDTP PEGLRLKAPR GWKADILQRE
KEEEEEDEEE GDRETTIRGK TTPAEQERVG KVKYSRLAKY FLNKEGDFNN LTSAAYHKKT
HLLVTGFASG IFHLHELPEF NLIHSLSISD QRVASVAINS SGDWIAFGCS GMGQLLVWEW
QSESYVLKQQ GHFNSMVALA YSPDGQYIVT GGDDGKVKVW NTLSGFCFVT LTEHSSGVTG
VTFTTTGHVI VTSSLDGTVR AFDLHRYRNF RTFTSPRPTQ FSCVAVDSSG EIVSAGAQDS
FEIFVWSMQT GRLLDVLSGH EGPVSGLCFN PMKSILASAS WDKTVRLWDM FDSWRTKETL
TLTSDALAVT FRPDGAELAV ATLNSQITFW DPENAVQVGS IEGRHDLKTG RKELDKITAK
HSAKGKAFTT LCYSADGQSI LAGGMSKFVC LYHVREQILV KRFELSCNLS LDAMEEFLNR
RKMTEFGNLA LIDQDAGEEN GVAVPLPGVR KGDMSSRHFK PEIRVTSLRF SPTGRCWAAT
STEGLLIFSL DAQMLFDPFE LDTSVTPGRI REALRQREFT RAILMAFRLN EKKLAQEALE
AVPQNEIEVV STSLPELYVV KVLEFLAASF EESRHLEFYL IWTQKLLMSH GQRLKSRAGQ
LLPVVQFLQK GLQRHLDDVS KLCDWNRFNI QYVLAVSKQR GMKRTLEPVD TEEDSDASDE
DSLHLLRAAG EEEEEEMLI
