ID   PXA1_SCHPO              Reviewed;         495 AA.
AC   O14200; P78842;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=PXA domain protein 1;
GN   Name=pxa1; ORFNames=SPAC5D6.07c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 175-495.
RC   STRAIN=PR745;
RX   PubMed=9501991; DOI=10.1093/dnares/4.6.363;
RA   Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.;
RT   "Identification of open reading frames in Schizosaccharomyces pombe
RT   cDNAs.";
RL   DNA Res. 4:363-369(1997).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=18256467; DOI=10.1271/bbb.70666;
RA   Hosomi A., Kawanishi Y.Y., Tanaka N., Takegawa K.;
RT   "PXA domain-containing protein Pxa1 is required for normal vacuole function
RT   and morphology in Schizosaccharomyces pombe.";
RL   Biosci. Biotechnol. Biochem. 72:548-556(2008).
CC   -!- FUNCTION: Required for required for normal vacuolar morphology and for
CC       vacuolar protein transport. Also required for endosome-to-Golgi protein
CC       transport. {ECO:0000269|PubMed:18256467}.
CC   -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000269|PubMed:18256467};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:18256467}.
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DR   EMBL; CU329670; CAB10855.1; -; Genomic_DNA.
DR   EMBL; D89191; BAA13853.1; -; mRNA.
DR   PIR; T38959; T38959.
DR   PIR; T42750; T42750.
DR   RefSeq; NP_593362.1; NM_001018794.2.
DR   AlphaFoldDB; O14200; -.
DR   SMR; O14200; -.
DR   STRING; 4896.SPAC5D6.07c.1; -.
DR   iPTMnet; O14200; -.
DR   MaxQB; O14200; -.
DR   PaxDb; O14200; -.
DR   PRIDE; O14200; -.
DR   EnsemblFungi; SPAC5D6.07c.1; SPAC5D6.07c.1:pep; SPAC5D6.07c.
DR   GeneID; 2541884; -.
DR   KEGG; spo:SPAC5D6.07c; -.
DR   PomBase; SPAC5D6.07c; pxa1.
DR   VEuPathDB; FungiDB:SPAC5D6.07c; -.
DR   eggNOG; ENOG502R9KS; Eukaryota.
DR   HOGENOM; CLU_551142_0_0_1; -.
DR   InParanoid; O14200; -.
DR   OMA; KGASERW; -.
DR   PRO; PR:O14200; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0005768; C:endosome; IDA:PomBase.
DR   GO; GO:0031303; C:integral component of endosome membrane; NAS:PomBase.
DR   GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IMP:PomBase.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0042147; P:retrograde transport, endosome to Golgi; IMP:PomBase.
DR   GO; GO:0007033; P:vacuole organization; IMP:PomBase.
DR   InterPro; IPR003114; Phox_assoc.
DR   Pfam; PF02194; PXA; 1.
DR   SMART; SM00313; PXA; 1.
DR   PROSITE; PS51207; PXA; 1.
PE   2: Evidence at transcript level;
KW   Endosome; Membrane; Protein transport; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..495
FT                   /note="PXA domain protein 1"
FT                   /id="PRO_0000116654"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        235..255
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        446..466
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          1..174
FT                   /note="PXA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00553"
FT   REGION          402..436
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        292
FT                   /note="S -> A (in Ref. 2; BAA13853)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   495 AA;  56508 MW;  4DE89F93C1E40FA7 CRC64;
     MAKLSSLLNP IISKILEIYV YSWYSGISKD ALFPSQCEQV GGSIVHELEK RLSRQDAMDL
     LFYEIPFLLI KHIENTEEAK LRFALPQGQI LEIDTIYHSL HPHIALEKEE NELVYCRLLV
     EDILKYLLPA TNSKSEIECV ILREALAVQI HKSIQVASSP ETMYKFIIYL SKAILQPSRR
     PWKESITTAV RWVWHAFRIL LITRGVPYFS TAWFQFYLKL FSQKDNVSSS DLTRWFFFYT
     LLYPWIALVS AFVAETMTLC CIVTIFYDKN VNRQWKQYIL TSVSNMDKGN PSGGSQSTNV
     TTFRRFSQSS YPRRSNYRRR ISTSSKSLYE LSPSKFKSIP ITSNPPPMLN LSKGSTSVEP
     TFCETNASVA LSTVTSTPVF STDSSPLSSR TRENLLSLIP SAVSSPTKAN TNKSHQRSFS
     IPKATKDSQT PSENSAATLK QAAIDAYSQI PVIPFFLPSD KLIMLVESEY RNKHIFYSLL
     NSFTMVMFPE LRHTK