PXA1_YEAST
ID PXA1_YEAST Reviewed; 870 AA.
AC P41909; D6W3M2;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 05-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Peroxisomal long-chain fatty acid import protein 2;
DE AltName: Full=Peroxisomal ABC transporter 1;
GN Name=PXA1; Synonyms=PAL1, PAT2, SSH2; OrderedLocusNames=YPL147W;
GN ORFNames=LPI1W, P2607;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=CH1305;
RX PubMed=7597071; DOI=10.1073/pnas.92.13.6012;
RA Shani N., Watkins P.A., Valle D.;
RT "PXA1, a possible Saccharomyces cerevisiae ortholog of the human
RT adrenoleukodystrophy gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:6012-6016(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8647887; DOI=10.1083/jcb.132.4.549;
RA Swartzman E.E., Viswanathan M.N., Thorner J.;
RT "The PAL1 gene product is a peroxisomal ATP-binding cassette transporter in
RT the yeast Saccharomyces cerevisiae.";
RL J. Cell Biol. 132:549-563(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=8948103;
RX DOI=10.1002/(sici)1097-0061(199611)12:14<1483::aid-yea34>3.0.co;2-o;
RA Purnelle B., Coster F., Goffeau A.;
RT "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a
RT small nuclear RNA, a new putative protein kinase and two new putative
RT regulators.";
RL Yeast 12:1483-1492(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP FUNCTION.
RX PubMed=8670886; DOI=10.1002/j.1460-2075.1996.tb00755.x;
RA Hettema E.H., van Roermund C.W.T., Distel B., van den Berg M., Vilela C.,
RA Rodrigues-Pousada C., Wanders R.J.A., Tabak H.F.;
RT "The ABC transporter proteins Pat1 and Pat2 are required for import of
RT long-chain fatty acids into peroxisomes of Saccharomyces cerevisiae.";
RL EMBO J. 15:3813-3822(1996).
CC -!- FUNCTION: Involved in the import of activated long-chain fatty acids
CC from the cytosol to the peroxisomal matrix.
CC {ECO:0000269|PubMed:8670886}.
CC -!- SUBUNIT: Forms a heterodimer with PXA2.
CC -!- INTERACTION:
CC P41909; P11076: ARF1; NbExp=2; IntAct=EBI-14344, EBI-2816;
CC P41909; P34230: PXA2; NbExp=2; IntAct=EBI-14344, EBI-2464632;
CC -!- SUBCELLULAR LOCATION: Peroxisome membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCD family.
CC Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB68215.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAC49009.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U17065; AAC49009.1; ALT_INIT; Genomic_DNA.
DR EMBL; L38491; AAC37480.1; -; Genomic_DNA.
DR EMBL; X96770; CAA65546.1; -; Genomic_DNA.
DR EMBL; U43703; AAB68215.1; ALT_INIT; Genomic_DNA.
DR EMBL; Z73503; CAA97852.1; -; Genomic_DNA.
DR EMBL; Z73502; CAA97851.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11288.1; -; Genomic_DNA.
DR PIR; S65158; S65158.
DR RefSeq; NP_015178.1; NM_001183961.1.
DR AlphaFoldDB; P41909; -.
DR SMR; P41909; -.
DR BioGRID; 36036; 113.
DR ComplexPortal; CPX-1730; Peroxisomal ABC transporter complex PXA1-PXA2.
DR DIP; DIP-7809N; -.
DR IntAct; P41909; 31.
DR MINT; P41909; -.
DR STRING; 4932.YPL147W; -.
DR TCDB; 3.A.1.203.6; the atp-binding cassette (abc) superfamily.
DR iPTMnet; P41909; -.
DR MaxQB; P41909; -.
DR PaxDb; P41909; -.
DR PRIDE; P41909; -.
DR EnsemblFungi; YPL147W_mRNA; YPL147W; YPL147W.
DR GeneID; 855956; -.
DR KEGG; sce:YPL147W; -.
DR SGD; S000006068; PXA1.
DR VEuPathDB; FungiDB:YPL147W; -.
DR eggNOG; KOG0064; Eukaryota.
DR GeneTree; ENSGT00950000182955; -.
DR HOGENOM; CLU_007587_1_1_1; -.
DR InParanoid; P41909; -.
DR OMA; IPRWNSK; -.
DR BioCyc; YEAST:G3O-34044-MON; -.
DR BRENDA; 7.6.2.4; 984.
DR Reactome; R-SCE-1369062; ABC transporters in lipid homeostasis.
DR Reactome; R-SCE-2046105; Linoleic acid (LA) metabolism.
DR Reactome; R-SCE-2046106; alpha-linolenic acid (ALA) metabolism.
DR Reactome; R-SCE-390247; Beta-oxidation of very long chain fatty acids.
DR Reactome; R-SCE-9603798; Class I peroxisomal membrane protein import.
DR PRO; PR:P41909; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P41909; protein.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IPI:ComplexPortal.
DR GO; GO:0005779; C:integral component of peroxisomal membrane; IDA:SGD.
DR GO; GO:0005778; C:peroxisomal membrane; IBA:GO_Central.
DR GO; GO:0005777; C:peroxisome; IDA:UniProtKB.
DR GO; GO:0140359; F:ABC-type transporter activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0042626; F:ATPase-coupled transmembrane transporter activity; IMP:SGD.
DR GO; GO:0005324; F:long-chain fatty acid transporter activity; IGI:UniProtKB.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IGI:UniProtKB.
DR GO; GO:0015908; P:fatty acid transport; IMP:SGD.
DR GO; GO:0015916; P:fatty-acyl-CoA transport; IGI:UniProtKB.
DR GO; GO:0042758; P:long-chain fatty acid catabolic process; IMP:UniProtKB.
DR GO; GO:0015910; P:long-chain fatty acid import into peroxisome; IDA:ComplexPortal.
DR GO; GO:0007031; P:peroxisome organization; IBA:GO_Central.
DR GO; GO:0042760; P:very long-chain fatty acid catabolic process; IMP:UniProtKB.
DR Gene3D; 1.20.1560.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011527; ABC1_TM_dom.
DR InterPro; IPR036640; ABC1_TM_sf.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR005283; FA_transporter.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF06472; ABC_membrane_2; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF90123; SSF90123; 1.
DR TIGRFAMs; TIGR00954; 3a01203; 1.
DR PROSITE; PS50929; ABC_TM1F; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Glycoprotein; Membrane; Nucleotide-binding; Peroxisome;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..870
FT /note="Peroxisomal long-chain fatty acid import protein 2"
FT /id="PRO_0000093314"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 210..501
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 608..866
FT /note="ABC transporter"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT BINDING 645..652
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 281
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 403
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 870 AA; 100025 MW; 45B20F888802E00A CRC64;
MSTTLAAPAK LKSLLLNLHT HCIGLHVNDV TPKVYFKLLI RHLLQISRSN AAHPKLRRRA
QILLVSLFLS GVTLFSGVTY STFKIILKCY KFYKFPWKRR NRRPLIRRTR SQMQLDSGAR
IMYIPEVELV DRQSPDDNKF MNATDKKKRK RIFIPPKDND VYEHDKFLFK NVELERAKNS
QLFYSKFLNQ MNVLSKILIP TVFDKNFLLL TAQIFFLVMR TWLSLFVAKL DGQIVKNIIA
GRGRSFLWDL GCWFLIAVPA SYTNSAIKLL QRKLSLNFRV NLTRYIHDMY LDKRLTFYKL
IFDAKASNSV IKNIDNSITN DVAKFCDATC SVFANIAKPV IDLIFFSVYL RDNLGTVGVA
GIFVNYFITG FILRKYTPPL GKLAGERSAS DGDYYNYHLN MINNSEEIAF YQGTAVERTK
VKELYDVLME KMLLVDKVKF GYNMLEDYVL KYTWSGLGYV FASIPIVMST LATGINSEEK
NMKEFIVNKR LMLSLADAGS RLMHSIKDIS QLTGYTNRIF TLLSVLHRVH SLNFNYGAVP
SILSIRTEDA SRNSNLLPTT DNSQDAIRGT IQRNFNGIRL ENIDVIIPSV RASEGIKLIN
KLTFQIPLHI DPITSKSNSI QDLSKANDIK LPFLQGSGSS LLILGPNGCG KSSIQRIIAE
IWPVYNKNGL LSIPSENNIF FIPQKPYFSR GGTLRDQIIY PMSSDEFFDR GFRDKELVQI
LVEVKLDYLL KRGVGLTYLD AIADWKDLLS GGEKQRVNFA RIMFHKPLYV VLDEATNAIS
VDMEDYLFNL LKRYRFNFIS ISQRPTLIKY HEMLLEIGEN RDGKWQLQAV GTDEAITSID
NEIEELERKL ERVKGWEDER TKLREKLEII
