ID   PXL2B_BOVIN             Reviewed;         201 AA.
AC   Q58CY6; A4FUH8;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Prostamide/prostaglandin F synthase;
DE            Short=Prostamide/PG F synthase;
DE            Short=Prostamide/PGF synthase;
DE            EC=1.11.1.20 {ECO:0000250|UniProtKB:Q9DB60};
DE   AltName: Full=Peroxiredoxin-like 2B;
GN   Name=PRXL2B; Synonyms=FAM213B;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal muscle;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reduction of prostaglandin-ethanolamide H(2)
CC       (prostamide H(2)) to prostamide F(2alpha) with NADPH as proton donor.
CC       Also able to reduce prostaglandin H(2) to prostaglandin F(2alpha) (By
CC       similarity). {ECO:0000250|UniProtKB:Q9DB60}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[thioredoxin]-dithiol + prostaglandin H2 = [thioredoxin]-
CC         disulfide + prostaglandin F2alpha; Xref=Rhea:RHEA:28214, Rhea:RHEA-
CC         COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:29950,
CC         ChEBI:CHEBI:50058, ChEBI:CHEBI:57404, ChEBI:CHEBI:57405;
CC         EC=1.11.1.20; Evidence={ECO:0000250|UniProtKB:Q9DB60};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[thioredoxin]-disulfide + prostamide F2alpha = [thioredoxin]-
CC         dithiol + prostamide H2; Xref=Rhea:RHEA:26373, Rhea:RHEA-COMP:10698,
CC         Rhea:RHEA-COMP:10700, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058,
CC         ChEBI:CHEBI:53081, ChEBI:CHEBI:53082; EC=1.11.1.20;
CC         Evidence={ECO:0000250|UniProtKB:Q9DB60};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q9DB60}.
CC   -!- SIMILARITY: Belongs to the peroxiredoxin-like PRXL2 family.
CC       Prostamide/prostaglandin F synthase subfamily. {ECO:0000305}.
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DR   EMBL; BT021811; AAX46658.1; -; mRNA.
DR   EMBL; BC114900; AAI14901.1; -; mRNA.
DR   RefSeq; NP_001035688.1; NM_001040598.1.
DR   AlphaFoldDB; Q58CY6; -.
DR   SMR; Q58CY6; -.
DR   STRING; 9913.ENSBTAP00000002262; -.
DR   PaxDb; Q58CY6; -.
DR   PRIDE; Q58CY6; -.
DR   GeneID; 617001; -.
DR   KEGG; bta:617001; -.
DR   CTD; 127281; -.
DR   eggNOG; KOG4498; Eukaryota.
DR   HOGENOM; CLU_094994_0_0_1; -.
DR   InParanoid; Q58CY6; -.
DR   OrthoDB; 1255928at2759; -.
DR   TreeFam; TF313804; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISS:CAFA.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:CAFA.
DR   GO; GO:0043209; C:myelin sheath; ISS:CAFA.
DR   GO; GO:0016209; F:antioxidant activity; IBA:GO_Central.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; ISS:UniProtKB.
DR   GO; GO:0047017; F:prostaglandin-F synthase activity; ISS:CAFA.
DR   GO; GO:0001516; P:prostaglandin biosynthetic process; ISS:UniProtKB.
DR   InterPro; IPR032801; PXL2A/B/C.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR28630; PTHR28630; 1.
DR   Pfam; PF13911; AhpC-TSA_2; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Lipid biosynthesis; Lipid metabolism; NADP; Oxidoreductase; Phosphoprotein;
KW   Prostaglandin biosynthesis; Prostaglandin metabolism; Reference proteome.
FT   CHAIN           1..201
FT                   /note="Prostamide/prostaglandin F synthase"
FT                   /id="PRO_0000284636"
FT   MOD_RES         108
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TBF2"
FT   CONFLICT        191
FT                   /note="L -> P (in Ref. 2; AAI14901)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   201 AA;  21497 MW;  BAD811DBA835263E CRC64;
     MSTVDLARVG ACVLKHAVTG EAVELRNLWQ EQACVVAGLR RFGCMVCRWI ARDLSNLKGL
     LDQHGVRLVG VGPEALGLQE FLDGGYFAGE LYLDESKQFY KELGFKRYNS LSILPAALGK
     PVREVAAKAK AVGIQGNLSG DLLQSGGLLV VAKGGDKVLL HFVQKSPGDY APLESILQAL
     GISAEVGPSE LPQCDEEACS R