ID   PXL2B_RAT               Reviewed;         201 AA.
AC   D3ZVR7;
DT   05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Prostamide/prostaglandin F synthase;
DE            Short=Prostamide/PG F synthase;
DE            Short=Prostamide/PGF synthase;
DE            EC=1.11.1.20 {ECO:0000250|UniProtKB:Q9DB60};
DE   AltName: Full=Peroxiredoxin-like 2B;
GN   Name=Prxl2b {ECO:0000312|RGD:1308251}; Synonyms=Fam213b;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the reduction of prostaglandin-ethanolamide H(2)
CC       (prostamide H(2)) to prostamide F(2alpha) with NADPH as proton donor.
CC       Also able to reduce prostaglandin H(2) to prostaglandin F(2alpha) (By
CC       similarity). {ECO:0000250|UniProtKB:Q9DB60}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[thioredoxin]-dithiol + prostaglandin H2 = [thioredoxin]-
CC         disulfide + prostaglandin F2alpha; Xref=Rhea:RHEA:28214, Rhea:RHEA-
CC         COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:29950,
CC         ChEBI:CHEBI:50058, ChEBI:CHEBI:57404, ChEBI:CHEBI:57405;
CC         EC=1.11.1.20; Evidence={ECO:0000250|UniProtKB:Q9DB60};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[thioredoxin]-disulfide + prostamide F2alpha = [thioredoxin]-
CC         dithiol + prostamide H2; Xref=Rhea:RHEA:26373, Rhea:RHEA-COMP:10698,
CC         Rhea:RHEA-COMP:10700, ChEBI:CHEBI:29950, ChEBI:CHEBI:50058,
CC         ChEBI:CHEBI:53081, ChEBI:CHEBI:53082; EC=1.11.1.20;
CC         Evidence={ECO:0000250|UniProtKB:Q9DB60};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q9DB60}.
CC   -!- SIMILARITY: Belongs to the peroxiredoxin-like PRXL2 family.
CC       Prostamide/prostaglandin F synthase subfamily. {ECO:0000305}.
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DR   EMBL; CH473968; EDL81278.1; -; Genomic_DNA.
DR   RefSeq; NP_001102167.1; NM_001108697.1.
DR   AlphaFoldDB; D3ZVR7; -.
DR   SMR; D3ZVR7; -.
DR   STRING; 10116.ENSRNOP00000018453; -.
DR   iPTMnet; D3ZVR7; -.
DR   PhosphoSitePlus; D3ZVR7; -.
DR   PaxDb; D3ZVR7; -.
DR   PeptideAtlas; D3ZVR7; -.
DR   PRIDE; D3ZVR7; -.
DR   GeneID; 362676; -.
DR   KEGG; rno:362676; -.
DR   CTD; 127281; -.
DR   RGD; 1308251; Prxl2b.
DR   eggNOG; KOG4498; Eukaryota.
DR   HOGENOM; CLU_094994_0_0_1; -.
DR   InParanoid; D3ZVR7; -.
DR   OMA; QRPVCND; -.
DR   OrthoDB; 1255928at2759; -.
DR   PhylomeDB; D3ZVR7; -.
DR   TreeFam; TF313804; -.
DR   PRO; PR:D3ZVR7; -.
DR   Proteomes; UP000002494; Chromosome 5.
DR   Proteomes; UP000234681; Chromosome 5.
DR   Bgee; ENSRNOG00000013468; Expressed in duodenum and 18 other tissues.
DR   Genevisible; D3ZVR7; RN.
DR   GO; GO:0005737; C:cytoplasm; ISS:CAFA.
DR   GO; GO:0005829; C:cytosol; ISO:RGD.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:CAFA.
DR   GO; GO:0043209; C:myelin sheath; IDA:UniProtKB.
DR   GO; GO:0016209; F:antioxidant activity; IBA:GO_Central.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; ISS:UniProtKB.
DR   GO; GO:0047017; F:prostaglandin-F synthase activity; ISS:CAFA.
DR   GO; GO:0001516; P:prostaglandin biosynthetic process; ISS:UniProtKB.
DR   InterPro; IPR032801; PXL2A/B/C.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR28630; PTHR28630; 1.
DR   Pfam; PF13911; AhpC-TSA_2; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Lipid biosynthesis; Lipid metabolism; NADP; Oxidoreductase; Phosphoprotein;
KW   Prostaglandin biosynthesis; Prostaglandin metabolism; Reference proteome.
FT   CHAIN           1..201
FT                   /note="Prostamide/prostaglandin F synthase"
FT                   /id="PRO_0000406969"
FT   MOD_RES         108
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8TBF2"
SQ   SEQUENCE   201 AA;  21607 MW;  D8FC444C21207494 CRC64;
     MSTLDLGRVG ACVLKHAVTG EAVELRSLWQ EKACVVAGLR RFGCMVCRWI AQDLSNLRGI
     LDQNDVRLVG IGPEALGLQE FLDGGYFSGE LYLDESKQIY KELGFKRYNS LSILPAALGK
     PVRDVASKAK AVGIQGNLSG DLLQSGGLLV VSKGGDRVLL HFIQSSPGDY VPQENILQAL
     GISAEVCSSK PPQCDEEVCG R