ID   PXPA2_PSEAE             Reviewed;         247 AA.
AC   Q9I203;
DT   04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=5-oxoprolinase subunit A 2 {ECO:0000255|HAMAP-Rule:MF_00691};
DE            Short=5-OPase subunit A 2 {ECO:0000255|HAMAP-Rule:MF_00691};
DE            EC=3.5.2.9 {ECO:0000255|HAMAP-Rule:MF_00691};
DE   AltName: Full=5-oxoprolinase (ATP-hydrolyzing) subunit A 2 {ECO:0000255|HAMAP-Rule:MF_00691};
GN   Name=pxpA2 {ECO:0000255|HAMAP-Rule:MF_00691}; OrderedLocusNames=PA2112;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes the cleavage of 5-oxoproline to form L-glutamate
CC       coupled to the hydrolysis of ATP to ADP and inorganic phosphate.
CC       {ECO:0000255|HAMAP-Rule:MF_00691}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-oxo-L-proline + ATP + 2 H2O = ADP + H(+) + L-glutamate +
CC         phosphate; Xref=Rhea:RHEA:10348, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58402, ChEBI:CHEBI:456216; EC=3.5.2.9;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00691};
CC   -!- SUBUNIT: Forms a complex composed of PxpA, PxpB and PxpC.
CC       {ECO:0000255|HAMAP-Rule:MF_00691}.
CC   -!- SIMILARITY: Belongs to the LamB/PxpA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00691}.
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DR   EMBL; AE004091; AAG05500.1; -; Genomic_DNA.
DR   PIR; G83382; G83382.
DR   RefSeq; NP_250802.1; NC_002516.2.
DR   RefSeq; WP_003113623.1; NZ_QZGE01000014.1.
DR   AlphaFoldDB; Q9I203; -.
DR   SMR; Q9I203; -.
DR   STRING; 287.DR97_5741; -.
DR   PaxDb; Q9I203; -.
DR   PRIDE; Q9I203; -.
DR   EnsemblBacteria; AAG05500; AAG05500; PA2112.
DR   GeneID; 879110; -.
DR   KEGG; pae:PA2112; -.
DR   PATRIC; fig|208964.12.peg.2202; -.
DR   PseudoCAP; PA2112; -.
DR   HOGENOM; CLU_069535_0_0_6; -.
DR   InParanoid; Q9I203; -.
DR   OMA; DRTYKQD; -.
DR   PhylomeDB; Q9I203; -.
DR   BioCyc; PAER208964:G1FZ6-2150-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0017168; F:5-oxoprolinase (ATP-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IBA:GO_Central.
DR   HAMAP; MF_00691; PxpA; 1.
DR   InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR   InterPro; IPR005501; LamB/YcsF/PxpA-like.
DR   PANTHER; PTHR30292; PTHR30292; 1.
DR   Pfam; PF03746; LamB_YcsF; 1.
DR   SUPFAM; SSF88713; SSF88713; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..247
FT                   /note="5-oxoprolinase subunit A 2"
FT                   /id="PRO_0000185026"
SQ   SEQUENCE   247 AA;  26334 MW;  310ECE26900540E6 CRC64;
     MNNLLLNCDI GESFGAWRMG LDAEVMPLID CANIACGFHA GDPGTMRRTV ALAVEHGVRI
     GAHPGYPDLV GFGRRSLACS AQEVEDLLLY QIGALDGLCR AQGTRVRYVK PHGALYQDMM
     RDPAILAAVL RALAAYDRTL PLMLMSTRDN TAARALGEAH GIELWFEAFA DRAYDGAGYL
     VSRSQPGAVH HDAETVLAQA LLLARGAALR ASDGSALALE AQTLCVHGDN PASLAAVRRI
     RDALEAA