PXPB_ECOLI
ID PXPB_ECOLI Reviewed; 218 AA.
AC P0AAV4; P75744;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=5-oxoprolinase subunit B {ECO:0000305};
DE Short=5-OPase subunit B {ECO:0000305};
DE EC=3.5.2.9 {ECO:0000269|PubMed:28830929};
DE AltName: Full=5-oxoprolinase (ATP-hydrolyzing) subunit B {ECO:0000305};
GN Name=pxpB {ECO:0000303|PubMed:28830929}; Synonyms=ybgJ;
GN OrderedLocusNames=b0711, JW0701;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, AND DISRUPTION PHENOTYPE.
RX PubMed=28830929; DOI=10.1074/jbc.m117.805028;
RA Niehaus T.D., Elbadawi-Sidhu M., de Crecy-Lagard V., Fiehn O., Hanson A.D.;
RT "Discovery of a widespread prokaryotic 5-oxoprolinase that was hiding in
RT plain sight.";
RL J. Biol. Chem. 292:16360-16367(2017).
RN [5] {ECO:0007744|PDB:5DUD}
RP X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS).
RA Arbing M.A., Kaufmann M., Shin A., Medrano-Soto A., Cascio D.,
RA Eisenberg D.;
RT "Crystal structure of E. coli YbgJK.";
RL Submitted (SEP-2015) to the PDB data bank.
CC -!- FUNCTION: Catalyzes the cleavage of 5-oxoproline to form L-glutamate
CC coupled to the hydrolysis of ATP to ADP and inorganic phosphate.
CC {ECO:0000269|PubMed:28830929}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-oxo-L-proline + ATP + 2 H2O = ADP + H(+) + L-glutamate +
CC phosphate; Xref=Rhea:RHEA:10348, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58402, ChEBI:CHEBI:456216; EC=3.5.2.9;
CC Evidence={ECO:0000269|PubMed:28830929};
CC -!- SUBUNIT: Forms a complex composed of PxpA, PxpB and PxpC.
CC {ECO:0000250|UniProtKB:P60495}.
CC -!- DISRUPTION PHENOTYPE: Deletion of the gene slows growth on minimal
CC medium with ammonium as nitrogen source. {ECO:0000269|PubMed:28830929}.
CC -!- SIMILARITY: Belongs to the PxpB family. {ECO:0000305}.
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DR EMBL; U00096; AAC73805.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35375.1; -; Genomic_DNA.
DR PIR; F64806; F64806.
DR RefSeq; NP_415239.1; NC_000913.3.
DR RefSeq; WP_001188343.1; NZ_STEB01000035.1.
DR PDB; 5DUD; X-ray; 2.80 A; B/D=1-218.
DR PDBsum; 5DUD; -.
DR AlphaFoldDB; P0AAV4; -.
DR SMR; P0AAV4; -.
DR BioGRID; 4259931; 194.
DR DIP; DIP-48145N; -.
DR IntAct; P0AAV4; 5.
DR STRING; 511145.b0711; -.
DR jPOST; P0AAV4; -.
DR PaxDb; P0AAV4; -.
DR PRIDE; P0AAV4; -.
DR EnsemblBacteria; AAC73805; AAC73805; b0711.
DR EnsemblBacteria; BAA35375; BAA35375; BAA35375.
DR GeneID; 66671020; -.
DR GeneID; 945311; -.
DR KEGG; ecj:JW0701; -.
DR KEGG; eco:b0711; -.
DR PATRIC; fig|1411691.4.peg.1562; -.
DR EchoBASE; EB3090; -.
DR eggNOG; COG2049; Bacteria.
DR HOGENOM; CLU_020207_0_0_6; -.
DR InParanoid; P0AAV4; -.
DR OMA; QPGFAYM; -.
DR PhylomeDB; P0AAV4; -.
DR BioCyc; EcoCyc:G6380-MON; -.
DR BioCyc; MetaCyc:G6380-MON; -.
DR PRO; PR:P0AAV4; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0017168; F:5-oxoprolinase (ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR Gene3D; 2.40.100.10; -; 1.
DR InterPro; IPR003833; CT_C_D.
DR InterPro; IPR029000; Cyclophilin-like_dom_sf.
DR InterPro; IPR010016; KipI_fam.
DR PANTHER; PTHR34698; PTHR34698; 1.
DR Pfam; PF02682; CT_C_D; 1.
DR SMART; SM00796; AHS1; 1.
DR SUPFAM; SSF50891; SSF50891; 1.
DR TIGRFAMs; TIGR00370; TIGR00370; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Hydrolase; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..218
FT /note="5-oxoprolinase subunit B"
FT /id="PRO_0000168706"
FT STRAND 4..9
FT /evidence="ECO:0007829|PDB:5DUD"
FT TURN 10..12
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 13..18
FT /evidence="ECO:0007829|PDB:5DUD"
FT HELIX 24..38
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 44..48
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 53..56
FT /evidence="ECO:0007829|PDB:5DUD"
FT HELIX 60..77
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 87..95
FT /evidence="ECO:0007829|PDB:5DUD"
FT TURN 96..98
FT /evidence="ECO:0007829|PDB:5DUD"
FT HELIX 102..109
FT /evidence="ECO:0007829|PDB:5DUD"
FT HELIX 113..122
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 125..130
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 137..139
FT /evidence="ECO:0007829|PDB:5DUD"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 162..166
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 169..173
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 181..187
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 196..201
FT /evidence="ECO:0007829|PDB:5DUD"
FT STRAND 207..213
FT /evidence="ECO:0007829|PDB:5DUD"
SQ SEQUENCE 218 AA; 23947 MW; 6AEBA50408732890 CRC64;
MQRARCYLIG ETAVVLELEP PVTLASQKRI WRLAQRLVDM PNVVEAIPGM NNITVILRNP
ESLALDAIER LQRWWEESEA LEPESRFIEI PVVYGGAGGP DLAVVAAHCG LSEKQVVELH
SSVEYVVWFL GFQPGFPYLG SLPEQLHTPR RAEPRLLVPA GSVGIGGPQT GVYPLATPGG
WQLIGHTSLS LFDPARDEPI LLRPGDSVRF VPQKEGVC
