PYCR2_BURCM
ID PYCR2_BURCM Reviewed; 311 AA.
AC Q0B953;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Delta(1)-pyrroline-2-carboxylate reductase 2;
DE Short=Pyr2C reductase 2;
DE EC=1.5.1.49 {ECO:0000269|PubMed:24980702};
DE AltName: Full=Proline ketimine reductase 2 {ECO:0000303|PubMed:24980702};
GN OrderedLocusNames=Bamb_3766 {ECO:0000312|EMBL:ABI89320.1};
OS Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia
OS (strain AMMD)).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=339670;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-244 / AMMD;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P.,
RA Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K.,
RA Ramette A., Tiedje J., Richardson P.;
RT "Complete sequence of chromosome 2 of Burkholderia cepacia AMMD.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=24980702; DOI=10.7554/elife.03275;
RA Zhao S., Sakai A., Zhang X., Vetting M.W., Kumar R., Hillerich B.,
RA San Francisco B., Solbiati J., Steves A., Brown S., Akiva E., Barber A.,
RA Seidel R.D., Babbitt P.C., Almo S.C., Gerlt J.A., Jacobson M.P.;
RT "Prediction and characterization of enzymatic activities guided by sequence
RT similarity and genome neighborhood networks.";
RL Elife 3:E03275-E03275(2014).
CC -!- FUNCTION: Catalyzes the reduction of Delta(1)-pyrroline-2-carboxylate
CC (Pyr2C) to L-proline, using preferentially NADPH over NADH as the
CC electron donor. May be involved in a degradation pathway that converts
CC trans-3-hydroxy-L-proline (t3LHyp) to L-proline.
CC {ECO:0000269|PubMed:24980702}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-proline + NAD(+) = 1-pyrroline-2-carboxylate + H(+) + NADH;
CC Xref=Rhea:RHEA:20321, ChEBI:CHEBI:15378, ChEBI:CHEBI:39785,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:60039; EC=1.5.1.49;
CC Evidence={ECO:0000269|PubMed:24980702};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-proline + NADP(+) = 1-pyrroline-2-carboxylate + H(+) +
CC NADPH; Xref=Rhea:RHEA:20317, ChEBI:CHEBI:15378, ChEBI:CHEBI:39785,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:60039; EC=1.5.1.49;
CC Evidence={ECO:0000269|PubMed:24980702};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.6 mM for Delta(1)-pyrroline-2-carboxylate (using NADPH as
CC cosubstrate) {ECO:0000269|PubMed:24980702};
CC KM=7.3 mM for Delta(1)-pyrroline-2-carboxylate (using NADH as
CC cosubstrate) {ECO:0000269|PubMed:24980702};
CC Note=kcat is 106 sec(-1) for Pyr2C reduction using NADPH. kcat is 41
CC sec(-1) for Pyr2C reduction using NADH.
CC {ECO:0000269|PubMed:24980702};
CC -!- SIMILARITY: Belongs to the ornithine cyclodeaminase/mu-crystallin
CC family. {ECO:0000305}.
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DR EMBL; CP000441; ABI89320.1; -; Genomic_DNA.
DR RefSeq; WP_011658776.1; NZ_CP009799.1.
DR AlphaFoldDB; Q0B953; -.
DR SMR; Q0B953; -.
DR STRING; 339670.Bamb_3766; -.
DR EnsemblBacteria; ABI89320; ABI89320; Bamb_3766.
DR GeneID; 44694402; -.
DR KEGG; bam:Bamb_3766; -.
DR PATRIC; fig|339670.21.peg.4010; -.
DR eggNOG; COG2423; Bacteria.
DR OMA; QARWQTR; -.
DR SABIO-RK; Q0B953; -.
DR Proteomes; UP000000662; Chromosome 2.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.1780.10; -; 1.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR003462; ODC_Mu_crystall.
DR InterPro; IPR023401; ODC_N.
DR PANTHER; PTHR13812; PTHR13812; 1.
DR Pfam; PF02423; OCD_Mu_crystall; 1.
DR PIRSF; PIRSF001439; CryM; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW NAD; NADP; Oxidoreductase.
FT CHAIN 1..311
FT /note="Delta(1)-pyrroline-2-carboxylate reductase 2"
FT /id="PRO_0000432302"
SQ SEQUENCE 311 AA; 32186 MW; 6D31701E8624E6B7 CRC64;
MTALSRIPAF DAAETAALLD YPALLATLTH TVAEYAAGEI VSPERLVVPL QGGGVMLSMP
SSARDLASHK LVNVCPGNAA RGLPTILGQV SAYDATTGEM RFVLDGPTVT GRRTAAITAL
GIQALHGAAP RDILLIGTGK QAANHAEALA AIFPDARLHV RGSRAGSAAA FCAAHRAQAP
QLAPLDGDAI PDAIDVVVTL TTSRTPVYRE AAREGRLVVG VGAFTADAAE IDADTVRHSR
LVVDDPAGAR HEAGDLIVAQ VDWQRVASLA DVLRGAFERS GPLLFKTVGC AAWDLAACRT
ARDALDARRG G
