ID   PYG1A_XENLA             Reviewed;         591 AA.
AC   Q5XHA8; Q642R7;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=CTP synthase 1-A {ECO:0000305};
DE            EC=6.3.4.2 {ECO:0000250|UniProtKB:P17812};
DE   AltName: Full=CTP synthetase 1-A;
DE   AltName: Full=UTP--ammonia ligase 1-A;
GN   Name=ctps1-a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Embryo, and Ovary;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: This enzyme is involved in the de novo synthesis of CTP, a
CC       precursor of DNA, RNA and phospholipids. Catalyzes the ATP-dependent
CC       amination of UTP to CTP with either L-glutamine or ammonia as a source
CC       of nitrogen. {ECO:0000250|UniProtKB:P17812}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-
CC         glutamate + phosphate; Xref=Rhea:RHEA:26426, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:37563, ChEBI:CHEBI:43474, ChEBI:CHEBI:46398,
CC         ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.4.2;
CC         Evidence={ECO:0000250|UniProtKB:P17812};
CC   -!- PATHWAY: Pyrimidine metabolism; CTP biosynthesis via de novo pathway;
CC       CTP from UDP: step 2/2. {ECO:0000250|UniProtKB:P17812}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q5XHA8-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q5XHA8-2; Sequence=VSP_019890, VSP_019891, VSP_019892;
CC   -!- SIMILARITY: Belongs to the CTP synthase family. {ECO:0000305}.
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DR   EMBL; BC081096; AAH81096.1; -; mRNA.
DR   EMBL; BC084164; AAH84164.1; -; mRNA.
DR   RefSeq; NP_001088219.1; NM_001094750.1. [Q5XHA8-1]
DR   AlphaFoldDB; Q5XHA8; -.
DR   SMR; Q5XHA8; -.
DR   BioGRID; 105127; 1.
DR   DNASU; 495047; -.
DR   GeneID; 495047; -.
DR   KEGG; xla:495047; -.
DR   CTD; 495047; -.
DR   Xenbase; XB-GENE-6254536; ctps1.S.
DR   OrthoDB; 810128at2759; -.
DR   UniPathway; UPA00159; UER00277.
DR   Proteomes; UP000186698; Chromosome 2S.
DR   Bgee; 495047; Expressed in neurula embryo and 19 other tissues.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003883; F:CTP synthase activity; ISS:UniProtKB.
DR   GO; GO:0044210; P:'de novo' CTP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006241; P:CTP biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd03113; CTPS_N; 1.
DR   CDD; cd01746; GATase1_CTP_Synthase; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_01227; PyrG; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR004468; CTP_synthase.
DR   InterPro; IPR017456; CTP_synthase_N.
DR   InterPro; IPR017926; GATASE.
DR   InterPro; IPR033828; GATase1_CTP_Synthase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11550; PTHR11550; 1.
DR   Pfam; PF06418; CTP_synth_N; 1.
DR   Pfam; PF00117; GATase; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00337; PyrG; 1.
DR   PROSITE; PS51273; GATASE_TYPE_1; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ATP-binding; Glutamine amidotransferase; Ligase;
KW   Nucleotide-binding; Pyrimidine biosynthesis; Reference proteome.
FT   CHAIN           1..591
FT                   /note="CTP synthase 1-A"
FT                   /id="PRO_0000247030"
FT   DOMAIN          300..554
FT                   /note="Glutamine amidotransferase type-1"
FT   ACT_SITE        399
FT                   /note="For GATase activity"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        526
FT                   /note="For GATase activity"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        528
FT                   /note="For GATase activity"
FT                   /evidence="ECO:0000250"
FT   VAR_SEQ         56..112
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_019890"
FT   VAR_SEQ         397..417
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_019891"
FT   VAR_SEQ         565..591
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_019892"
FT   CONFLICT        365
FT                   /note="E -> D (in Ref. 1; AAH81096)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   591 AA;  66872 MW;  9A1140CA053223E7 CRC64;
     MKYILVTGGV ISGIGKGVIA SSIGTILKSS NLHVTSIKID PYINIDAGTF SPYEHGEVFV
     LDDGGEVDLD LGNYERFLDI RLTKDNNLTT GKIYQSVINK ERKGDYLGKT VQVVPHITDA
     IQEWVMRQAL IPVDEDGIEP EVCVIELGGT VGDIESMPFV EAFRQFQFKV RRENFCNIHV
     SLVPQPSATG EQKTKPTQNS VRELRGLGLS PDLVVCRCST PLDTSVKEKI SMFCHVEPQQ
     VICVHDVSSI YRVPLLLEEQ GVVDYFRQRL DLPIGRQPRR LLMKWKEMAD RYERLLESCS
     IALVGKYTKF SDSYASVIKA LEHSALAINH RLEIKYIDSA DLEQETLQEE PVRYHEAWQK
     LCSSEGILVP GGFGVRGTEG KIQAIAWARK QKKPFLGVCL GMQLAVVEFA RDVLGWTDAN
     STEFNPKTSH PVVIDMPEHN PGQMGGTMRL GKRRTIFHSQ NSIMKKLYGG HEYVEERHRH
     RYEVNPELRR ELEVRGLKFV GQDTEGERME IVELEDHPYF VGVQYHPEFL SRPIKPSPPY
     FGLLLASVGR LSQYIERGCR LSPRDTYSDR SENSSPDAEI AELKLPMIDH D