PYG7_ARATH
ID PYG7_ARATH Reviewed; 301 AA.
AC B9DHG0; O80551; Q8LFF4; Q94EJ1;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Tetratricopeptide repeat domain-containing protein PYG7, chloroplastic {ECO:0000305};
DE AltName: Full=Protein PALE YELLOW GREEN 7 {ECO:0000303|PubMed:16679416};
DE Flags: Precursor;
GN Name=PYG7 {ECO:0000303|PubMed:16679416};
GN OrderedLocusNames=At1g22700 {ECO:0000312|Araport:AT1G22700};
GN ORFNames=T22J18.13 {ECO:0000312|EMBL:AAC25516.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION BY LIGHT, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=16679416; DOI=10.1104/pp.106.078147;
RA Stoeckel J., Bennewitz S., Hein P., Oelmueller R.;
RT "The evolutionarily conserved tetratrico peptide repeat protein pale yellow
RT green7 is required for photosystem I accumulation in Arabidopsis and
RT copurifies with the complex.";
RL Plant Physiol. 141:870-878(2006).
RN [7]
RP FUNCTION, AND INTERACTION WITH PSA3.
RX PubMed=28522455; DOI=10.1104/pp.17.00524;
RA Shen J., Williams-Carrier R., Barkan A.;
RT "PSA3, a protein on the stromal face of the thylakoid membrane, promotes
RT photosystem I accumulation in cooperation with the assembly factor PYG7.";
RL Plant Physiol. 174:1850-1862(2017).
CC -!- FUNCTION: Nuclear genome-encoded factor required for the accumulation
CC of photosystem I (PSI). Functions as PSI biogenesis factor
CC (PubMed:16679416, PubMed:28522455). Cooperates with PSA3 to promote the
CC stable assembly of PSI in the thylakoid membrane. May target primarily
CC the PsaC subunit (PubMed:28522455). {ECO:0000269|PubMed:16679416,
CC ECO:0000269|PubMed:28522455}.
CC -!- SUBUNIT: Interacts with PSA3. {ECO:0000269|PubMed:28522455}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:16679416}; Multi-pass membrane protein
CC {ECO:0000255}. Note=Copurifies with PSI. {ECO:0000269|PubMed:16679416}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=B9DHG0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B9DHG0-2; Sequence=VSP_059018;
CC -!- INDUCTION: Induced by light. {ECO:0000269|PubMed:16679416}.
CC -!- DISRUPTION PHENOTYPE: Seedling lethality when homozygous. Dwarf and
CC pale yellowish phenotype when grown on MS medium supplemented with 2%
CC sucrose. {ECO:0000269|PubMed:16679416}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC25516.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC003979; AAC25516.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30273.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30274.1; -; Genomic_DNA.
DR EMBL; AF410279; AAK95265.1; -; mRNA.
DR EMBL; BT001148; AAN64539.1; -; mRNA.
DR EMBL; AK317512; BAH20177.1; -; mRNA.
DR EMBL; AY084882; AAM61445.1; -; mRNA.
DR PIR; T00774; T00774.
DR RefSeq; NP_564178.2; NM_102117.5. [B9DHG0-2]
DR RefSeq; NP_850950.1; NM_180619.2. [B9DHG0-1]
DR AlphaFoldDB; B9DHG0; -.
DR SMR; B9DHG0; -.
DR STRING; 3702.AT1G22700.1; -.
DR iPTMnet; B9DHG0; -.
DR PaxDb; B9DHG0; -.
DR PRIDE; B9DHG0; -.
DR ProteomicsDB; 224796; -. [B9DHG0-1]
DR EnsemblPlants; AT1G22700.1; AT1G22700.1; AT1G22700. [B9DHG0-1]
DR EnsemblPlants; AT1G22700.2; AT1G22700.2; AT1G22700. [B9DHG0-2]
DR GeneID; 838876; -.
DR Gramene; AT1G22700.1; AT1G22700.1; AT1G22700. [B9DHG0-1]
DR Gramene; AT1G22700.2; AT1G22700.2; AT1G22700. [B9DHG0-2]
DR KEGG; ath:AT1G22700; -.
DR Araport; AT1G22700; -.
DR TAIR; locus:2199639; AT1G22700.
DR eggNOG; ENOG502QW5C; Eukaryota.
DR InParanoid; B9DHG0; -.
DR OrthoDB; 950085at2759; -.
DR PhylomeDB; B9DHG0; -.
DR PRO; PR:B9DHG0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; B9DHG0; baseline and differential.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0048564; P:photosystem I assembly; IMP:TAIR.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR001440; TPR_1.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF00515; TPR_1; 1.
DR SMART; SM00028; TPR; 2.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS50005; TPR; 3.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Membrane; Plastid; Reference proteome;
KW Repeat; Thylakoid; TPR repeat; Transit peptide; Transmembrane;
KW Transmembrane helix.
FT TRANSIT 1..61
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 62..301
FT /note="Tetratricopeptide repeat domain-containing protein
FT PYG7, chloroplastic"
FT /id="PRO_0000440981"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 168..201
FT /note="TPR 1"
FT /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT ProRule:PRU00339"
FT REPEAT 206..239
FT /note="TPR 2"
FT /evidence="ECO:0000255, ECO:0000255|PROSITE-
FT ProRule:PRU00339"
FT REPEAT 240..273
FT /note="TPR 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT VAR_SEQ 54..58
FT /note="Missing (in isoform 2)"
FT /id="VSP_059018"
FT CONFLICT 180
FT /note="Missing (in Ref. 5; AAM61445)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 301 AA; 33662 MW; D4F56F973AE694C8 CRC64;
MFESNMVLQT LSSSSPPIHR LYLHHSQILP SSGSPSKISL QIHGRTLAIR SFHDYVFAEI
SARGLPALNK ASLKKLPIKG STFLLGQSLL MVSAHPQLAA AAEIIKPEPI YEVGELFELS
IQLSYLLLLL GLLGVGTFYV IRQVLVRREL DLSAKELQEQ VRSGDASATE LFELGAVMLR
RKFYPAANKF LQQAIQKWDG DDQDLAQVYN ALGVSYVRED KLDKGIAQFE MAVKLQPGYV
TAWNNLGDAY EKKKELPLAL NAFEEVLLFD PNNKVARPRR DALKDRVKLY KGVVAVKSKK
R
