ID   PYG_SYNY3               Reviewed;         249 AA.
AC   P73093;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   17-JUN-2020, sequence version 2.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Phycobilisome rod-core linker polypeptide CpcG;
GN   Name=cpcG; Synonyms=cpcG1 {ECO:0000303|PubMed:16049785};
GN   OrderedLocusNames=slr2051;
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC   unclassified Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-20.
RX   PubMed=9298645; DOI=10.1002/elps.1150180806;
RA   Sazuka T., Ohara O.;
RT   "Towards a proteome project of cyanobacterium Synechocystis sp. strain
RT   PCC6803: linking 130 protein spots with their respective genes.";
RL   Electrophoresis 18:1252-1258(1997).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-11, AND SUBUNIT.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=31015331; DOI=10.1128/mbio.00669-19;
RA   Liu H., Weisz D.A., Zhang M.M., Cheng M., Zhang B., Zhang H.,
RA   Gerstenecker G.S., Pakrasi H.B., Gross M.L., Blankenship R.E.;
RT   "Phycobilisomes Harbor FNRL in Cyanobacteria.";
RL   MBio 10:0-0(2019).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=16049785; DOI=10.1007/s11120-004-7762-9;
RA   Kondo K., Geng X.X., Katayama M., Ikeuchi M.;
RT   "Distinct roles of CpcG1 and CpcG2 in phycobilisome assembly in the
RT   cyanobacterium Synechocystis sp. PCC 6803.";
RL   Photosyn. Res. 84:269-273(2005).
RN   [5]
RP   FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=17468217; DOI=10.1104/pp.107.099267;
RA   Kondo K., Ochiai Y., Katayama M., Ikeuchi M.;
RT   "The membrane-associated CpcG2-phycobilisome in Synechocystis: a new
RT   photosystem I antenna.";
RL   Plant Physiol. 144:1200-1210(2007).
RN   [6]
RP   DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=19152018; DOI=10.1007/s11120-008-9399-6;
RA   Kondo K., Mullineaux C.W., Ikeuchi M.;
RT   "Distinct roles of CpcG1-phycobilisome and CpcG2-phycobilisome in state
RT   transitions in a cyanobacterium Synechocystis sp. PCC 6803.";
RL   Photosyn. Res. 99:217-225(2009).
CC   -!- FUNCTION: Rod-core linker protein required for attachment of rods with
CC       the core of the major allophycocyanins in phycobilisomes, plays a major
CC       role in light harvesting. Although able to transfer energy to both
CC       photosystems, this is predominantly a PSII antenna (PubMed:16049785,
CC       PubMed:17468217, PubMed:19152018). Involved in state transitions
CC       (PubMed:19152018). {ECO:0000269|PubMed:16049785,
CC       ECO:0000269|PubMed:17468217, ECO:0000269|PubMed:19152018}.
CC   -!- SUBUNIT: Part of the phycobilisome, a hemidiscoidal structure that is
CC       composed of two distinct substructures: a core complex and a number of
CC       rods radiating from the core. This protein is part of a conventional
CC       PBS rod (called CpcG-PBS) with on average 3 stacked phycocyanin
CC       hexamers (PC, CpcA and CpcB). Linker CpcG connects the PC stack to the
CC       thylakoid, the hexamers are linked by 1 copy of CpcC1, 1 copy of CpcC2
CC       and the stack is terminated by a single copy of CpcD. Ferredoxin--NADP
CC       reductase (petH) is also part of the complex.
CC       {ECO:0000269|PubMed:17468217}.
CC   -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane
CC       {ECO:0000269|PubMed:17468217}; Peripheral membrane protein
CC       {ECO:0000305|PubMed:17468217}; Cytoplasmic side
CC       {ECO:0000305|PubMed:17468217}. Note=The phycobilisome supercomplex
CC       detaches from the thylakoid easily. {ECO:0000269|PubMed:17468217}.
CC   -!- DISRUPTION PHENOTYPE: Decreased growth rate, decreased energy transfer
CC       to photosystem II. Decreased accumulation of phycocyanin (PC), loss of
CC       the phycobilisome supercomplex and major phycobiliproteins ApcE and
CC       allophycocyanin. A double cpcG1/cpcG2 deletion has even less PC, but
CC       grows slightly better than this single mutant (PubMed:16049785,
CC       PubMed:17468217). Unable to perform state 2 to state 1 transitions
CC       (i.e. cannot transfer energy to PSII) (PubMed:19152018).
CC       {ECO:0000269|PubMed:16049785, ECO:0000269|PubMed:17468217,
CC       ECO:0000269|PubMed:19152018}.
CC   -!- SIMILARITY: Belongs to the phycobilisome linker protein family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00775}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA17118.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; BA000022; BAA17118.1; ALT_INIT; Genomic_DNA.
DR   PIR; S75204; S75204.
DR   AlphaFoldDB; P73093; -.
DR   SMR; P73093; -.
DR   IntAct; P73093; 7.
DR   STRING; 1148.1652194; -.
DR   PaxDb; P73093; -.
DR   EnsemblBacteria; BAA17118; BAA17118; BAA17118.
DR   KEGG; syn:slr2051; -.
DR   eggNOG; COG0448; Bacteria.
DR   PhylomeDB; P73093; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0030089; C:phycobilisome; IEA:UniProtKB-KW.
DR   GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015979; P:photosynthesis; IEA:InterPro.
DR   Gene3D; 1.10.3130.20; -; 1.
DR   InterPro; IPR001297; PBS_linker_dom.
DR   InterPro; IPR038255; PBS_linker_sf.
DR   InterPro; IPR016470; Phycobilisome.
DR   Pfam; PF00427; PBS_linker_poly; 1.
DR   PIRSF; PIRSF005898; Phycobilisome_CpeC/CpcI; 1.
DR   PROSITE; PS51445; PBS_LINKER; 1.
PE   1: Evidence at protein level;
KW   Antenna complex; Direct protein sequencing; Membrane; Phycobilisome;
KW   Reference proteome; Thylakoid.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:31015331,
FT                   ECO:0000269|PubMed:9298645"
FT   CHAIN           2..249
FT                   /note="Phycobilisome rod-core linker polypeptide CpcG"
FT                   /id="PRO_0000449924"
FT   DOMAIN          11..188
FT                   /note="PBS-linker"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00775"
FT   CONFLICT        12..13
FT                   /note="SQ -> QK (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   249 AA;  28902 MW;  C1CD6E5DCDB6DFFC CRC64;
     MALPLLNYAP KSQNVRVEGY EIGSEEKPVV FTTENILSSS DMDNLIEAAY RQIFFHAFKW
     DREKVLESQL RNGQITVRDF VRGLLLSNTF RNSFYEKNSN YRFVEHCVQK ILGRDVYSER
     EKIAWSIVVA TKGYQGLIDD LLNSDEYLNN FGYDTVPYQR RRNLPGREAG ELPFNIKSPR
     YDAYHRRQLG FPQIVWQNEV RRFIPQEKKL TAGNPMNFLG MARSINPAAN TIPKVSAQNI
     NIEASVPRR