PYHD_NPVAC
ID PYHD_NPVAC Reviewed; 245 AA.
AC P04871; Q64813;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 23-FEB-2022, entry version 81.
DE RecName: Full=Polyhedrin;
DE AltName: Full=Major occlusion protein;
GN Name=PH; Synonyms=P29, POLH;
OS Autographa californica nuclear polyhedrosis virus (AcMNPV).
OC Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX NCBI_TaxID=46015;
OH NCBI_TaxID=7088; Lepidoptera (butterflies and moths).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=E2;
RA Hooft van Iddekinge B.J.L., Smith G.E., Summers M.D.;
RT "Nucleotide sequence of the polyhedrin gene of Autographa californica
RT nuclear polyhedrosis virus.";
RL Virology 131:561-565(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C6;
RX PubMed=1926775; DOI=10.1016/0042-6822(91)90770-c;
RA Possee R.D., Sun T.P., Howard S.C., Ayres M.D., Hill-Perkins M.,
RA Gearing K.L.;
RT "Nucleotide sequence of the Autographa californica nuclear polyhedrosis 9.4
RT kbp EcoRI-I and -R (polyhedrin gene) region.";
RL Virology 185:229-241(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C6;
RX PubMed=8030224; DOI=10.1006/viro.1994.1380;
RA Ayres M.D., Howard S.C., Kuzio J., Lopez-Ferber M., Possee R.D.;
RT "The complete DNA sequence of Autographa californica nuclear polyhedrosis
RT virus.";
RL Virology 202:586-605(1994).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-85.
RC STRAIN=L1;
RX PubMed=6325875; DOI=10.1128/mcb.4.3.399-406.1984;
RA Pennock G.D., Shoemaker C., Miller L.K.;
RT "Strong and regulated expression of Escherichia coli beta-galactosidase in
RT insect cells with a baculovirus vector.";
RL Mol. Cell. Biol. 4:399-406(1984).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-18 AND 209-245.
RA Howard S.C., Ayres M.D., Possee R.D.;
RT "Mapping the 5' and 3' ends of Autographa californica nuclear polyhedrosis
RT virus polyhedrin mRNA.";
RL Virus Res. 5:109-119(1986).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-72.
RC STRAIN=C6;
RX PubMed=2407804; DOI=10.1099/0022-1317-71-2-251;
RA Gearing K.L., Possee R.D.;
RT "Functional analysis of a 603 nucleotide open reading frame upstream of the
RT polyhedrin gene of Autographa californica nuclear polyhedrosis virus.";
RL J. Gen. Virol. 71:251-262(1990).
RN [7]
RP MUTAGENESIS OF LEU-85.
RX PubMed=19731454; DOI=10.1099/0022-1317-68-3-901;
RA Carstens E.B., Ye L.B., Faulkner P.;
RT "A point mutation in the polyhedrin gene of a baculovirus, Autographa
RT californica MNPV, prevents crystallization of occlusion bodies.";
RL J. Gen. Virol. 68:901-905(1987).
RN [8]
RP SUBCELLULAR LOCATION, AND NUCLEAR LOCALIZATION SIGNAL.
RX PubMed=1962451; DOI=10.1016/0042-6822(91)90551-l;
RA Jarvis D.L., Bohlmeyer D.A., Garcia A. Jr.;
RT "Requirements for nuclear localization and supramolecular assembly of a
RT baculovirus polyhedrin protein.";
RL Virology 185:795-810(1991).
RN [9]
RP FUNCTION, AND MUTAGENESIS OF LEU-183.
RX PubMed=1607865; DOI=10.1099/0022-1317-73-6-1471;
RA Carstens E.B., Williams G.V., Faulkner P., Partington S.;
RT "Analysis of polyhedra morphology mutants of Autographa californica nuclear
RT polyhedrosis virus: molecular and ultrastructural features.";
RL J. Gen. Virol. 73:1471-1479(1992).
RN [10]
RP FUNCTION, AND MUTAGENESIS OF GLY-25.
RX PubMed=10963398; DOI=10.1006/jipa.2000.4934;
RA Lin G.Y., Zhong J., Wang X.Z.;
RT "Abnormal formation of polyhedra resulting from a single mutation in the
RT polyhedrin gene of Autographa californica multicapsid
RT nucleopolyhedrovirus.";
RL J. Invertebr. Pathol. 76:13-19(2000).
RN [11]
RP MUTAGENESIS OF VAL-118.
RX PubMed=19038296; DOI=10.1016/j.virusres.2008.10.010;
RA Ribeiro B.M., Generino A.P., Acacio C.N., Kalapothakis E., Bao S.N.;
RT "Characterization of a new Autographa californica multiple
RT nucleopolyhedrovirus (AcMNPV) polyhedra mutant.";
RL Virus Res. 140:1-7(2009).
RN [12]
RP X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS).
RX PubMed=19959989; DOI=10.1038/emboj.2009.352;
RA Ji X., Sutton G., Evans G., Axford D., Owen R., Stuart D.I.;
RT "How baculovirus polyhedra fit square pegs into round holes to robustly
RT package viruses.";
RL EMBO J. 29:505-514(2010).
RN [13]
RP X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS).
RX PubMed=20007786; DOI=10.1073/pnas.0910686106;
RA Coulibaly F., Chiu E., Gutmann S., Rajendran C., Haebel P.W., Ikeda K.,
RA Mori H., Ward V.K., Schulze-Briese C., Metcalf P.;
RT "The atomic structure of baculovirus polyhedra reveals the independent
RT emergence of infectious crystals in DNA and RNA viruses.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:22205-22210(2009).
CC -!- FUNCTION: Major component of the virus occlusion bodies which are large
CC proteinaceous structures termed polyhedra. These structures serve as
CC the protective package for the virus particles outside the infected
CC host and allow natural transmission of virus between insect hosts,
CC assisting persistence in the environment. Forms the paracrystalline
CC lattice of polyhedra and interacts with enveloped virions as well as
CC other accessory molecules and structures to form a mature viral
CC occlusion body. {ECO:0000269|PubMed:1607865}.
CC -!- INTERACTION:
CC P04871; P04871: PH; NbExp=4; IntAct=EBI-7644439, EBI-7644439;
CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:1962451}.
CC -!- SIMILARITY: Belongs to the polyhedrin family. {ECO:0000305}.
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DR EMBL; K01149; AAA46719.1; -; Genomic_DNA.
DR EMBL; M75679; AAA46704.1; -; Genomic_DNA.
DR EMBL; K02700; AAA46722.1; -; Genomic_DNA.
DR EMBL; M21887; AAA46720.1; -; Genomic_DNA.
DR EMBL; M24120; AAA46721.1; -; Genomic_DNA.
DR EMBL; D00700; BAA00607.1; -; Genomic_DNA.
DR EMBL; L22858; AAA66638.1; -; Genomic_DNA.
DR PIR; A03803; PYNVA2.
DR RefSeq; NP_054037.1; NC_001623.1.
DR PDB; 2WUX; X-ray; 1.84 A; A=1-245.
DR PDB; 2WUY; X-ray; 3.09 A; A=1-245.
DR PDB; 3JW6; X-ray; 2.30 A; A=1-245.
DR PDBsum; 2WUX; -.
DR PDBsum; 2WUY; -.
DR PDBsum; 3JW6; -.
DR SMR; P04871; -.
DR DIP; DIP-49027N; -.
DR MINT; P04871; -.
DR GeneID; 1403840; -.
DR KEGG; vg:1403840; -.
DR EvolutionaryTrace; P04871; -.
DR Proteomes; UP000008292; Genome.
DR GO; GO:0044204; C:host cell nuclear matrix; IDA:UniProtKB.
DR GO; GO:0039679; C:viral occlusion body; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR InterPro; IPR001746; Polyhedrin.
DR Pfam; PF00738; Polyhedrin; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Host nucleus; Reference proteome; Viral occlusion body.
FT CHAIN 1..245
FT /note="Polyhedrin"
FT /id="PRO_0000217243"
FT REGION 32..35
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:1962451"
FT MUTAGEN 25
FT /note="G->D: Forms a single large polyhedron."
FT /evidence="ECO:0000269|PubMed:10963398"
FT MUTAGEN 85
FT /note="L->F: Prevents crystallization of occlusion bodies."
FT /evidence="ECO:0000269|PubMed:19731454"
FT MUTAGEN 118
FT /note="V->F: Forms few or no polyhedra and accumulates with
FT a dispersed granular protein mass."
FT /evidence="ECO:0000269|PubMed:19038296"
FT MUTAGEN 183
FT /note="L->F: Prevents the occlusion of virus particles."
FT /evidence="ECO:0000269|PubMed:1607865"
FT STRAND 13..16
FT /evidence="ECO:0007829|PDB:2WUX"
FT STRAND 19..23
FT /evidence="ECO:0007829|PDB:2WUX"
FT HELIX 24..28
FT /evidence="ECO:0007829|PDB:2WUX"
FT HELIX 50..52
FT /evidence="ECO:0007829|PDB:2WUX"
FT STRAND 54..56
FT /evidence="ECO:0007829|PDB:2WUX"
FT TURN 59..61
FT /evidence="ECO:0007829|PDB:2WUX"
FT STRAND 62..78
FT /evidence="ECO:0007829|PDB:2WUX"
FT STRAND 82..85
FT /evidence="ECO:0007829|PDB:2WUX"
FT HELIX 91..106
FT /evidence="ECO:0007829|PDB:2WUX"
FT STRAND 111..130
FT /evidence="ECO:0007829|PDB:2WUX"
FT STRAND 135..143
FT /evidence="ECO:0007829|PDB:2WUX"
FT STRAND 151..154
FT /evidence="ECO:0007829|PDB:2WUX"
FT STRAND 166..170
FT /evidence="ECO:0007829|PDB:2WUX"
FT HELIX 191..196
FT /evidence="ECO:0007829|PDB:2WUX"
FT HELIX 199..202
FT /evidence="ECO:0007829|PDB:2WUX"
FT STRAND 205..213
FT /evidence="ECO:0007829|PDB:2WUX"
FT STRAND 218..233
FT /evidence="ECO:0007829|PDB:2WUX"
SQ SEQUENCE 245 AA; 28642 MW; F5F5FE9A9BF8189E CRC64;
MPDYSYRPTI GRTYVYDNKY YKNLGAVIKN AKRKKHFAEH EIEEATLDPL DNYLVAEDPF
LGPGKNQKLT LFKEIRNVKP DTMKLVVGWK GKEFYRETWT RFMEDSFPIV NDQEVMDVFL
VVNMRPTRPN RCYKFLAQHA LRCDPDYVPH DVIRIVEPSW VGSNNEYRIS LAKKGGGCPI
MNLHSEYTNS FEQFIDRVIW ENFYKPIVYI GTDSAEEEEI LLEVSLVFKV KEFAPDAPLF
TGPAY
