ID   PYIR_MAGGR              Reviewed;         611 AA.
AC   A0A4P8W7L7;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   31-JUL-2019, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Pyrichalasin H cluster regulator pyiR {ECO:0000303|PubMed:31099577};
DE   AltName: Full=Pyrichalasin H biosynthesis cluster protein R {ECO:0000303|PubMed:31099577};
GN   Name=pyiR {ECO:0000303|PubMed:31099577};
OS   Magnaporthe grisea (Crabgrass-specific blast fungus) (Pyricularia grisea).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=148305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=NI980;
RX   PubMed=31099577; DOI=10.1021/acs.orglett.9b01344;
RA   Wang C., Hantke V., Cox R.J., Skellam E.;
RT   "Targeted gene inactivations expose silent cytochalasans in Magnaporthe
RT   grisea NI980.";
RL   Org. Lett. 21:4163-4167(2019).
RN   [2]
RP   FUNCTION.
RX   PubMed=31644300; DOI=10.1021/acs.orglett.9b03372;
RA   Wang C., Becker K., Pfuetze S., Kuhnert E., Stadler M., Cox R.J.,
RA   Skellam E.;
RT   "Investigating the function of cryptic cytochalasan cytochrome P450
RT   monooxygenases using combinatorial biosynthesis.";
RL   Org. Lett. 21:8756-8760(2019).
CC   -!- FUNCTION: Transcription factor that specifically regulates the
CC       expression of the gene cluster that mediates the biosynthesis of the
CC       mycotoxin pyrichalasin H, a tyrosine-derived cytochalasan that inhibits
CC       the growth of rice seedlings, but also inhibits lymphocyte capping and
CC       actin polymerization and alters cell morphology (PubMed:31099577)
CC       (Probable). Pyrichalasin H is indicated as the responsible agent for
CC       the genus-specific pathogenicity of M.grisea toward crabgrass
CC       (PubMed:31099577). {ECO:0000269|PubMed:31099577,
CC       ECO:0000305|PubMed:31644300}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
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DR   EMBL; MK801691; QCS37518.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A4P8W7L7; -.
DR   Proteomes; UP000515153; Genome assembly.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..611
FT                   /note="Pyrichalasin H cluster regulator pyiR"
FT                   /id="PRO_0000449445"
FT   DNA_BIND        11..47
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          53..128
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          169..192
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          265..291
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          401..427
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          521..550
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          564..593
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        75..114
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   611 AA;  65336 MW;  4175ED14EE15CABE CRC64;
     MHRFNPRRSA CDRCRGHKLR CIRLDPGPND TGALLPCKRC VKAGAECIHT ANLSVKPPGD
     GHHSAHRATE SPGHPAAFQQ ETRVSDRQLR ESSLGISPTQ PAPQRQTQRW SSSSGPHRPE
     DRESLPPWHM FATPMFSRQQ LGFSKLPSHA NGPPDDRTAD GFEVGSEAAL AAPPGSERSP
     HRTSRAPSDG TTTFSLVDKV FDLDGRAVCP LSGSVLQENA GIVTAAASTT VTSAQTHHML
     PFSQVEPLTP SSYFNVPTCS TLTTGGAGSQ SLRDQQMQQH QRHGSASGRS SAATQDSCLQ
     LLSQLSSKFL MDFGKSSAGD WSKMANNNTN NHLSTTISNL FDGLQIFLKT IECLRPATFL
     ENSSSDSECS YSDLCDESEF VGSTGDNQMQ VYPGAMAVDH AHEGSSTENS AHRRGRGASP
     TADAAPQPLD MPMTLTILTC YTWLLKGYEV VLSEIYQMLA SQDRHQGLQT LPTIVQGVGI
     GGFKLEDHPD MQIEIVIHVG WQLLQRIEGL LGVRVVSDEG RGGLGGSSRD ESSTEGGDGG
     GAGSSASDER RILDPRAAAA VLDSWFTTTT RGGSGGSGPG EGTGDSNGGR TVEIKGTIAN
     IRKYLRSYGR N