ID   PYLS_METBF              Reviewed;         419 AA.
AC   Q46E77;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Pyrrolysine--tRNA ligase;
DE            EC=6.1.1.26;
DE   AltName: Full=Pyrrolysine--tRNA(Pyl) ligase;
DE   AltName: Full=Pyrrolysyl-tRNA synthetase;
DE            Short=PylRS;
GN   Name=pylS; OrderedLocusNames=Mbar_A0839;
OS   Methanosarcina barkeri (strain Fusaro / DSM 804).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=269797;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Fusaro / DSM 804;
RX   PubMed=16980466; DOI=10.1128/jb.00810-06;
RA   Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S.,
RA   Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R.;
RT   "The Methanosarcina barkeri genome: comparative analysis with
RT   Methanosarcina acetivorans and Methanosarcina mazei reveals extensive
RT   rearrangement within methanosarcinal genomes.";
RL   J. Bacteriol. 188:7922-7931(2006).
RN   [2]
RP   FUNCTION.
RX   PubMed=15314242; DOI=10.1073/pnas.0405362101;
RA   Polycarpo C., Ambrogelly A., Berube A., Winbush S.M., McCloskey J.A.,
RA   Crain P.F., Wood J.L., Soell D.;
RT   "An aminoacyl-tRNA synthetase that specifically activates pyrrolysine.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:12450-12454(2004).
CC   -!- FUNCTION: Catalyzes the attachment of pyrrolysine to tRNA(Pyl).
CC       Pyrrolysine is a lysine derivative encoded by the termination codon
CC       UAG. {ECO:0000269|PubMed:15314242}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-
CC         pyrrolysyl-tRNA(Pyl); Xref=Rhea:RHEA:19277, Rhea:RHEA-COMP:9720,
CC         Rhea:RHEA-COMP:9721, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:58499, ChEBI:CHEBI:78442, ChEBI:CHEBI:78556,
CC         ChEBI:CHEBI:456215; EC=6.1.1.26;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; CP000099; AAZ69815.1; -; Genomic_DNA.
DR   RefSeq; WP_011305865.1; NC_007355.1.
DR   AlphaFoldDB; Q46E77; -.
DR   SMR; Q46E77; -.
DR   STRING; 269797.Mbar_A0839; -.
DR   EnsemblBacteria; AAZ69815; AAZ69815; Mbar_A0839.
DR   GeneID; 3625577; -.
DR   KEGG; mba:Mbar_A0839; -.
DR   eggNOG; arCOG00413; Archaea.
DR   HOGENOM; CLU_648316_0_0_2; -.
DR   OMA; RPMLAPN; -.
DR   OrthoDB; 25314at2157; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0043767; F:pyrrolysyl-tRNA synthetase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0006418; P:tRNA aminoacylation for protein translation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.930.10; -; 1.
DR   HAMAP; MF_01573; Pyl_tRNA_synth; 1.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR002319; Phenylalanyl-tRNA_Synthase.
DR   InterPro; IPR012739; Pyrrolysyl-tRNA_ligase.
DR   InterPro; IPR023877; Pyrrolysyl-tRNA_ligase_C.
DR   InterPro; IPR023878; Pyrrolysyl-tRNA_ligase_N.
DR   Pfam; PF01409; tRNA-synt_2d; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   TIGRFAMs; TIGR02367; PylS_Cterm; 1.
DR   TIGRFAMs; TIGR03912; PylS_Nterm; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..419
FT                   /note="Pyrrolysine--tRNA ligase"
FT                   /id="PRO_0000260450"
FT   REGION          101..157
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        123..139
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   419 AA;  47690 MW;  3AA51387F53F8350 CRC64;
     MDKKPLDVLI SATGLWMSRT GTLHKIKHYE VSRSKIYIEM ACGDHLVVNN SRSCRTARAF
     RHHKYRKTCK RCRVSDEDIN NFLTRSTEGK TSVKVKVVSA PKVKKAMPKS VSRAPKPLEN
     PVSAKASTDT SRSVPSPAKS TPNSPVPTSA PAPSLTRSQL DRVEALLSPE DKISLNIAKP
     FRELESELVT RRKNDFQRLY TNDREDYLGK LERDITKFFV DRDFLEIKSP ILIPAEYVER
     MGINNDTELS KQIFRVDKNL CLRPMLAPTL YNYLRKLDRI LPDPIKIFEV GPCYRKESDG
     KEHLEEFTMV NFCQMGSGCT RENLESLIKE FLDYLEIDFE IVGDSCMVYG DTLDIMHGDL
     ELSSAVVGPV PLDREWGIDK PWIGAGFGLE RLLKVMHGFK NIKRASRSES YYNGISTNL