PYM1_DANRE
ID PYM1_DANRE Reviewed; 194 AA.
AC Q6PH11; Q6NVC8;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Partner of Y14 and mago {ECO:0000250|UniProtKB:P82804};
DE AltName: Full=PYM homolog 1 exon junction complex-associated factor {ECO:0000250|UniProtKB:Q9BRP8};
DE AltName: Full=Protein wibg homolog;
GN Name=pym1 {ECO:0000250|UniProtKB:Q9BRP8}; Synonyms=pym, wibg;
GN ORFNames=zgc:65891;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo, and Testis;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Key regulator of the exon junction complex (EJC), a
CC multiprotein complex that associates immediately upstream of the exon-
CC exon junction on mRNAs and serves as a positional landmark for the
CC intron exon structure of genes and directs post-transcriptional
CC processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA
CC decay (NMD) or translation. Acts as an EJC disassembly factor, allowing
CC translation-dependent EJC removal and recycling by disrupting mature
CC EJC from spliced mRNAs. Its association with the 40S ribosomal subunit
CC probably prevents a translation-independent disassembly of the EJC from
CC spliced mRNAs, by restricting its activity to mRNAs that have been
CC translated. Interferes with NMD and enhances translation of spliced
CC mRNAs, probably by antagonizing EJC functions (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts (via N-terminus) with magoh and rbm8a; the
CC interaction is direct. Associates (eIF2A-like region) with the 40S
CC ribosomal subunit and the 48S preinitiation complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BRP8}.
CC Nucleus, nucleolus {ECO:0000250|UniProtKB:Q9BRP8}. Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q9BRP8}. Note=Shuttles between the nucleus and
CC the cytoplasm. Nuclear export is mediated by XPO1/CRM1.
CC {ECO:0000250|UniProtKB:Q9BRP8}.
CC -!- SIMILARITY: Belongs to the pym family. {ECO:0000305}.
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DR EMBL; BC056755; AAH56755.1; -; mRNA.
DR EMBL; BC068187; AAH68187.1; -; mRNA.
DR EMBL; BC100118; AAI00119.1; -; mRNA.
DR RefSeq; NP_956888.2; NM_200594.2.
DR AlphaFoldDB; Q6PH11; -.
DR SMR; Q6PH11; -.
DR STRING; 7955.ENSDARP00000067667; -.
DR PaxDb; Q6PH11; -.
DR Ensembl; ENSDART00000167361; ENSDARP00000136874; ENSDARG00000046024.
DR GeneID; 393566; -.
DR KEGG; dre:393566; -.
DR CTD; 84305; -.
DR ZFIN; ZDB-GENE-040426-1464; pym1.
DR eggNOG; KOG4325; Eukaryota.
DR GeneTree; ENSGT00730000111107; -.
DR InParanoid; Q6PH11; -.
DR OrthoDB; 1545729at2759; -.
DR PhylomeDB; Q6PH11; -.
DR TreeFam; TF324615; -.
DR PRO; PR:Q6PH11; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 8.
DR Bgee; ENSDARG00000046024; Expressed in mature ovarian follicle and 21 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0035145; C:exon-exon junction complex; ISS:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0043022; F:ribosome binding; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:1903259; P:exon-exon junction complex disassembly; IBA:GO_Central.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; ISS:UniProtKB.
DR GO; GO:0045727; P:positive regulation of translation; ISS:UniProtKB.
DR InterPro; IPR039333; PYM1.
DR InterPro; IPR015362; WIBG_mago-bd.
DR InterPro; IPR036348; WIBG_N_sf.
DR PANTHER; PTHR22959; PTHR22959; 1.
DR Pfam; PF09282; Mago-bind; 1.
DR SMART; SM01273; Mago-bind; 1.
DR SUPFAM; SSF101931; SSF101931; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Cytoplasm; Nonsense-mediated mRNA decay; Nucleus;
KW Reference proteome; Translation regulation.
FT CHAIN 1..194
FT /note="Partner of Y14 and mago"
FT /id="PRO_0000287287"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 54..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 137..192
FT /evidence="ECO:0000255"
FT COMPBIAS 64..79
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..108
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..134
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 102
FT /note="S -> P (in Ref. 1; AAH68187)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 194 AA; 22066 MW; B3010A21866CC700 CRC64;
MATPYVTDES GKYIAATQRP DGSWRKPRRV RDGYVPQEEV PVYENKFVKF FKSKPELPPG
VCVETPPQTQ TQPSDAAGLS RTAKRNMKRK EKRRQQGQET KSEPELQPEP ELQPEPEPQG
LSQQMQQLEL SASQGPGAAD SARRLKNLRK KLRQVEELQQ RVLSGELKPS QEQLDKLGRA
QALREELQQL EAHS
