PYM_ARATH
ID PYM_ARATH Reviewed; 259 AA.
AC O48533;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Protein POLYCHOME;
DE AltName: Full=Protein UV-B-INSENSITIVE 4;
GN Name=PYM; Synonyms=UVI4; OrderedLocusNames=At2g42260; ORFNames=T24P15.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10224275; DOI=10.1093/genetics/152.1.461;
RA Perazza D., Herzog M., Huelskamp M., Brown S., Dorne A.-M.,
RA Bonneville J.-M.;
RT "Trichome cell growth in Arabidopsis thaliana can be derepressed by
RT mutations in at least five genes.";
RL Genetics 152:461-476(1999).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=16623893; DOI=10.1111/j.1365-313x.2006.02696.x;
RA Hase Y., Trung K.H., Matsunaga T., Tanaka A.;
RT "A mutation in the uvi4 gene promotes progression of endo-reduplication and
RT confers increased tolerance towards ultraviolet B light.";
RL Plant J. 46:317-326(2006).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH FZR2; FZR3; CDC20.1
RP AND CDC20.5.
RX PubMed=22167058; DOI=10.1105/tpc.111.092049;
RA Iwata E., Ikeda S., Matsunaga S., Kurata M., Yoshioka Y., Criqui M.-C.,
RA Genschik P., Ito M.;
RT "GIGAS CELL1, a novel negative regulator of the anaphase-promoting
RT complex/cyclosome, is required for proper mitotic progression and cell fate
RT determination in Arabidopsis.";
RL Plant Cell 23:4382-4393(2011).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 64-ARG--LEU-67;
RP 92-GLY--ASN-95; 119-ARG--LEU-122 AND 258-MET-ARG-259, INDUCTION,
RP INTERACTION WITH FZR2 AND APC5, AND SUBCELLULAR LOCATION.
RX PubMed=22167059; DOI=10.1105/tpc.111.091793;
RA Heyman J., Van den Daele H., De Wit K., Boudolf V., Berckmans B.,
RA Verkest A., Alvim Kamei C.L., De Jaeger G., Koncz C., De Veylder L.;
RT "Arabidopsis ULTRAVIOLET-B-INSENSITIVE4 maintains cell division activity by
RT temporal inhibition of the anaphase-promoting complex/cyclosome.";
RL Plant Cell 23:4394-4410(2011).
RN [9]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22899078; DOI=10.4161/psb.21133;
RA Iwata E., Ikeda S., Abe N., Kobayashi A., Kurata M., Matsunaga S.,
RA Yoshioka Y., Criqui M.C., Genschik P., Ito M.;
RT "Roles of GIG1 and UVI4 in genome duplication in Arabidopsis thaliana.";
RL Plant Signal. Behav. 7:1079-1081(2012).
RN [10]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=23345424; DOI=10.1073/pnas.1217024110;
RA Bao Z., Yang H., Hua J.;
RT "Perturbation of cell cycle regulation triggers plant immune response via
RT activation of disease resistance genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:2407-2412(2013).
CC -!- FUNCTION: Negative regulator of the anaphase-promoting
CC complex/cyclosome (APC/C) ubiquitin ligase required for proper mitotic
CC progression and cell fate determination; inhibits premature cell
CC differentiation. Prevents DNA endoreplication by promoting the
CC maintenance of the mitotic state by preferentially inhibiting
CC APC/C(FZR) and triggering cyclins accumulation (e.g. CYCB1-1, CYCB1-2
CC and CYCA2-3) in a temporal manner. Required for megagametophyte and
CC endosperm development. Counteracts the activity of CCS52A1 thus
CC inhibiting the turnover of CYCA2-3. Confers immunity to bacterial
CC pathogens (e.g. Pseudomonas syringae pv. tomato DC3000), which is
CC associated with increased expression of disease resistance (R) genes.
CC {ECO:0000269|PubMed:10224275, ECO:0000269|PubMed:16623893,
CC ECO:0000269|PubMed:22167058, ECO:0000269|PubMed:22167059,
CC ECO:0000269|PubMed:22899078, ECO:0000269|PubMed:23345424}.
CC -!- SUBUNIT: Interacts with APC/C activators such as APC5, FZR2, FZR3,
CC CDC20.1 and CDC20.5. {ECO:0000269|PubMed:22167058,
CC ECO:0000269|PubMed:22167059}.
CC -!- INTERACTION:
CC O48533; O23160: MYB73; NbExp=3; IntAct=EBI-2130722, EBI-25506855;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22167059}.
CC Note=Follows a patchy nuclear accumulation pattern.
CC -!- TISSUE SPECIFICITY: Expressed mainly in actively dividing cells (e.g.
CC central cylinder of the root tip, young leaves and vascular tissues).
CC {ECO:0000269|PubMed:16623893}.
CC -!- DEVELOPMENTAL STAGE: Levels fade progressively in a basipetal fashion
CC as the leaf develops. {ECO:0000269|PubMed:16623893}.
CC -!- INDUCTION: Expressed in an S phase-dependent fashion.
CC {ECO:0000269|PubMed:22167059}.
CC -!- DISRUPTION PHENOTYPE: Premature occurrence of endoreplication during
CC organ development leading to an increased DNA content in hypocotyls and
CC trichomes associated with an increased branching of trichomes (e.g.
CC four to five branches). Reduced accumulation of CYCA2-3 during the S
CC phase leading to a premature exit from the cell cycle, thus triggering
CC the onset of the endocycle. Increased tolerance towards ultraviolet B
CC light (UVB), probably due to the enhanced polyploidization.
CC Considerably larger average cell area in the leaf adaxial epidermis
CC leading to fewer but larger epidermal cells.
CC {ECO:0000269|PubMed:10224275, ECO:0000269|PubMed:16623893,
CC ECO:0000269|PubMed:22167058, ECO:0000269|PubMed:22167059,
CC ECO:0000269|PubMed:22899078}.
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DR EMBL; AC002561; AAB88650.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10097.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62271.1; -; Genomic_DNA.
DR EMBL; BT005212; AAO63276.1; -; mRNA.
DR EMBL; AK227846; BAE99823.1; -; mRNA.
DR PIR; T00935; T00935.
DR RefSeq; NP_001324441.1; NM_001336961.1.
DR RefSeq; NP_181755.1; NM_129788.3.
DR AlphaFoldDB; O48533; -.
DR SMR; O48533; -.
DR BioGRID; 4164; 21.
DR IntAct; O48533; 21.
DR STRING; 3702.AT2G42260.1; -.
DR PaxDb; O48533; -.
DR PRIDE; O48533; -.
DR EnsemblPlants; AT2G42260.1; AT2G42260.1; AT2G42260.
DR EnsemblPlants; AT2G42260.2; AT2G42260.2; AT2G42260.
DR GeneID; 818827; -.
DR Gramene; AT2G42260.1; AT2G42260.1; AT2G42260.
DR Gramene; AT2G42260.2; AT2G42260.2; AT2G42260.
DR KEGG; ath:AT2G42260; -.
DR Araport; AT2G42260; -.
DR TAIR; locus:2060035; AT2G42260.
DR eggNOG; ENOG502RZ6Y; Eukaryota.
DR HOGENOM; CLU_078386_0_0_1; -.
DR InParanoid; O48533; -.
DR OMA; PEWYPRS; -.
DR OrthoDB; 1487532at2759; -.
DR PhylomeDB; O48533; -.
DR PRO; PR:O48533; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O48533; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0042023; P:DNA endoreduplication; IMP:TAIR.
DR GO; GO:0009960; P:endosperm development; IMP:UniProtKB.
DR GO; GO:0009561; P:megagametogenesis; IMP:UniProtKB.
DR GO; GO:1904667; P:negative regulation of ubiquitin protein ligase activity; IMP:UniProtKB.
DR GO; GO:1900426; P:positive regulation of defense response to bacterium; IMP:UniProtKB.
DR GO; GO:0051783; P:regulation of nuclear division; IEA:InterPro.
DR GO; GO:0010224; P:response to UV-B; IMP:TAIR.
DR GO; GO:0010091; P:trichome branching; IMP:TAIR.
DR InterPro; IPR034590; POLYCHOME/GIG1.
DR PANTHER; PTHR35119; PTHR35119; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Developmental protein; DNA replication; Mitosis;
KW Nucleus; Plant defense; Reference proteome.
FT CHAIN 1..259
FT /note="Protein POLYCHOME"
FT /id="PRO_0000423305"
FT REGION 236..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 64..67
FT /note="RGGL->AGGA: Can complement disruption phenotype."
FT /evidence="ECO:0000269|PubMed:22167059"
FT MUTAGEN 92..95
FT /note="GREN->ARAA: Impaired interaction with FZR2. Cannot
FT complement disruption phenotype."
FT /evidence="ECO:0000269|PubMed:22167059"
FT MUTAGEN 119..122
FT /note="RTPL->ATPA: Impaired interaction with FZR2. Can
FT complement disruption phenotype."
FT /evidence="ECO:0000269|PubMed:22167059"
FT MUTAGEN 258..259
FT /note="Missing: Impaired interaction with FZR2. Cannot
FT complement disruption phenotype."
FT /evidence="ECO:0000269|PubMed:22167059"
SQ SEQUENCE 259 AA; 28731 MW; D6A973F22DD569C4 CRC64;
MPEARDRIER QVDYPAAFLN RRSHGILLDE PATQHNLFGS PVQRVPSEAT GGLGSIGQGS
MTGRGGLVRG NFGIRRTGGG RRGQIQFRSP QGRENMSLGV TRRGRARASN SVLPSWYPRT
PLRDISAVVR AIERRRARMG EGVGRDIETP TPQQLGVLDS LVPLSGAHLE HDYSMVTPGP
SIGFKRPWPP STAKVHQILL DITRENTGEE DALTPEKKLL NSIDKVEKVV MEEIQKMKST
PSAKRAEREK RVRTLMSMR
