ID   PYRB3_PEA               Reviewed;         391 AA.
AC   Q43064;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Aspartate carbamoyltransferase 3, chloroplastic;
DE            EC=2.1.3.2;
DE   AltName: Full=Aspartate transcarbamylase 3;
DE            Short=ATCase 3;
DE   Flags: Precursor;
GN   Name=PYRB3;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Wando; TISSUE=Leaf;
RA   Williamson C.L., To L., Slocum R.D.;
RT   "Characterization of a cDNA encoding a third aspartate transcarbamoylase
RT   (pyrB3) from pea.";
RL   (er) Plant Gene Register PGR96-063(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-
CC         aspartate + phosphate; Xref=Rhea:RHEA:20013, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29991, ChEBI:CHEBI:32814, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:58228; EC=2.1.3.2;
CC   -!- ACTIVITY REGULATION: Allosterically regulated by UMP.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC       (S)-dihydroorotate from bicarbonate: step 2/3.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the aspartate/ornithine carbamoyltransferase
CC       superfamily. ATCase family. {ECO:0000305}.
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DR   EMBL; U05293; AAB67857.1; -; mRNA.
DR   PIR; T06425; T06425.
DR   AlphaFoldDB; Q43064; -.
DR   SMR; Q43064; -.
DR   PRIDE; Q43064; -.
DR   EnsemblPlants; Psat7g089200.1; Psat7g089200.1.cds; Psat7g089200.
DR   EnsemblPlants; Psat7g089200.2; Psat7g089200.2.cds; Psat7g089200.
DR   Gramene; Psat7g089200.1; Psat7g089200.1.cds; Psat7g089200.
DR   Gramene; Psat7g089200.2; Psat7g089200.2.cds; Psat7g089200.
DR   UniPathway; UPA00070; UER00116.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0016597; F:amino acid binding; IEA:InterPro.
DR   GO; GO:0004070; F:aspartate carbamoyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006520; P:cellular amino acid metabolic process; IEA:InterPro.
DR   Gene3D; 3.40.50.1370; -; 2.
DR   HAMAP; MF_00001; Asp_carb_tr; 1.
DR   InterPro; IPR006132; Asp/Orn_carbamoyltranf_P-bd.
DR   InterPro; IPR006130; Asp/Orn_carbamoylTrfase.
DR   InterPro; IPR036901; Asp/Orn_carbamoylTrfase_sf.
DR   InterPro; IPR002082; Asp_carbamoyltransf.
DR   InterPro; IPR006131; Asp_carbamoyltransf_Asp/Orn-bd.
DR   Pfam; PF00185; OTCace; 1.
DR   Pfam; PF02729; OTCace_N; 1.
DR   PRINTS; PR00100; AOTCASE.
DR   SUPFAM; SSF53671; SSF53671; 1.
DR   TIGRFAMs; TIGR00670; asp_carb_tr; 1.
DR   PROSITE; PS00097; CARBAMOYLTRANSFERASE; 1.
PE   2: Evidence at transcript level;
KW   Allosteric enzyme; Chloroplast; Plastid; Pyrimidine biosynthesis;
KW   Transferase; Transit peptide.
FT   TRANSIT         1..69
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           70..391
FT                   /note="Aspartate carbamoyltransferase 3, chloroplastic"
FT                   /id="PRO_0000020351"
SQ   SEQUENCE   391 AA;  44344 MW;  5693EBC634FAEBA9 CRC64;
     MTASSSLFSC SMHMEVLTPK ISKWPKDFVS CHSKISYVET NYLKSTCYPI SRFLCINNLS
     KCDKMIKTRQ RDGIHCFSEG QKFQLDDVIE AQQFDRDILN AIFEIARDME NIERNSPESQ
     ILKGYLMATL FYEPSTRTRL SFESAMRRLG GEVLTTENAR EFSSAAKGET LEDTIRTVEG
     YSDLIVLRHF ESGAARRAAA IAGIPIVNAG DGPGQHPSQA LLDVYTIERE IGKLDGIKVG
     LVGDLANGRT VRSLAYLLAK YKDVKIYFVS PEVVKMKDDI KDYLTSKGVD WEESSDLVEV
     ASECDVVYQT RIQKERFGER LDLYEKARGK FIVNQNILNA MQRHAVIMHP LPRLDEITVD
     VDADPRAAYF RQAKYGLYIR MALLKLLLVG W