PYRB_LACLM
ID PYRB_LACLM Reviewed; 310 AA.
AC Q9L4N6; A2RJN4;
DT 25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Aspartate carbamoyltransferase {ECO:0000255|HAMAP-Rule:MF_00001};
DE EC=2.1.3.2 {ECO:0000255|HAMAP-Rule:MF_00001};
DE AltName: Full=Aspartate transcarbamylase {ECO:0000255|HAMAP-Rule:MF_00001};
DE Short=ATCase {ECO:0000255|HAMAP-Rule:MF_00001};
GN Name=pyrB {ECO:0000255|HAMAP-Rule:MF_00001}; OrderedLocusNames=llmg_0893;
OS Lactococcus lactis subsp. cremoris (strain MG1363).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus; Lactococcus cremoris subsp. cremoris.
OX NCBI_TaxID=416870;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11292797; DOI=10.1128/jb.183.9.2785-2794.2001;
RA Martinussen J., Schallert J., Andersen B., Hammer K.;
RT "The pyrimidine operon pyrRPB-carA from Lactococcus lactis.";
RL J. Bacteriol. 183:2785-2794(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MG1363;
RX PubMed=17307855; DOI=10.1128/jb.01768-06;
RA Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C.,
RA Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P.,
RA van Sinderen D., Kok J.;
RT "The complete genome sequence of the lactic acid bacterial paradigm
RT Lactococcus lactis subsp. cremoris MG1363.";
RL J. Bacteriol. 189:3256-3270(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-
CC aspartate + phosphate; Xref=Rhea:RHEA:20013, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29991, ChEBI:CHEBI:32814, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:58228; EC=2.1.3.2; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00001};
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC (S)-dihydroorotate from bicarbonate: step 2/3. {ECO:0000255|HAMAP-
CC Rule:MF_00001}.
CC -!- SIMILARITY: Belongs to the aspartate/ornithine carbamoyltransferase
CC superfamily. ATCase family. {ECO:0000255|HAMAP-Rule:MF_00001}.
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DR EMBL; AJ132624; CAB89871.1; -; Genomic_DNA.
DR EMBL; AM406671; CAL97487.1; -; Genomic_DNA.
DR RefSeq; WP_011834851.1; NZ_WJVF01000036.1.
DR AlphaFoldDB; Q9L4N6; -.
DR SMR; Q9L4N6; -.
DR STRING; 416870.llmg_0893; -.
DR EnsemblBacteria; CAL97487; CAL97487; llmg_0893.
DR KEGG; llm:llmg_0893; -.
DR eggNOG; COG0540; Bacteria.
DR HOGENOM; CLU_043846_2_1_9; -.
DR OMA; FPTEREY; -.
DR PhylomeDB; Q9L4N6; -.
DR BioCyc; LLAC416870:LLMG_RS04560-MON; -.
DR UniPathway; UPA00070; UER00116.
DR Proteomes; UP000000364; Chromosome.
DR GO; GO:0016597; F:amino acid binding; IEA:InterPro.
DR GO; GO:0004070; F:aspartate carbamoyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006520; P:cellular amino acid metabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.1370; -; 2.
DR HAMAP; MF_00001; Asp_carb_tr; 1.
DR InterPro; IPR006132; Asp/Orn_carbamoyltranf_P-bd.
DR InterPro; IPR006130; Asp/Orn_carbamoylTrfase.
DR InterPro; IPR036901; Asp/Orn_carbamoylTrfase_sf.
DR InterPro; IPR002082; Asp_carbamoyltransf.
DR InterPro; IPR006131; Asp_carbamoyltransf_Asp/Orn-bd.
DR PANTHER; PTHR11405:SF16; PTHR11405:SF16; 1.
DR Pfam; PF00185; OTCace; 1.
DR Pfam; PF02729; OTCace_N; 1.
DR PRINTS; PR00100; AOTCASE.
DR SUPFAM; SSF53671; SSF53671; 1.
DR TIGRFAMs; TIGR00670; asp_carb_tr; 1.
DR PROSITE; PS00097; CARBAMOYLTRANSFERASE; 1.
PE 3: Inferred from homology;
KW Pyrimidine biosynthesis; Transferase.
FT CHAIN 1..310
FT /note="Aspartate carbamoyltransferase"
FT /id="PRO_0000113146"
SQ SEQUENCE 310 AA; 34558 MW; DA6C688ECA9F803E CRC64;
MSVKNGLVQL ENLTSMENLS IDEVMGLIKR ASAFKAGKAD FALDEQTFAS NLFFENSTRT
HHSFHIAERK LGLDVLEFDA QASSISKGET LYDTVLTLDA LGVDICVIRS GVEHYYEELV
NSDNIHCAIV NGGDGSGQHP SQCLLDLMTI YEEFGKFEGL KIAISGDLTH SRVAKSNMMM
LQKLGARLYF TGPAAWYSEE FDDYGHYANL DRILPELDVH MLLRVQHERH DSGESFSKEG
YHTHFGLTQE RAKMLKPTAI IMHPAPVNRD VEIADSLVES PQSRIVQQMS NGVYTRMAIL
EAILAGKKAK