ID   ATP78_ECOLX             Reviewed;         550 AA.
AC   P0DV91;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Retron Ec78 probable ATPase {ECO:0000303|PubMed:9281493};
GN   ORFNames=Ga0100609_101823;
OS   Escherichia coli.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1] {ECO:0000312|EMBL:JHRW01000018}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=102598;
RA   Hazen T.H., Donnenberg M., Nataro J., Kaper J., Rasko D.;
RT   "Emergence of novel diverse phylogenomic lineages of EPEC.";
RL   Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000312|EMBL:U02551}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-293.
RC   STRAIN=Clinical strain 110;
RX   PubMed=9281493; DOI=10.1006/plas.1997.1298;
RA   Lima T.M., Lim D.;
RT   "A novel retron that produces RNA-less msDNA in Escherichia coli using
RT   reverse transcriptase.";
RL   Plasmid 38:25-33(1997).
RN   [3]
RP   FUNCTION IN ANTIVIRAL DEFENSE, AND IDENTIFICATION AS A RETRON.
RC   STRAIN=102598;
RX   PubMed=33157039; DOI=10.1016/j.cell.2020.09.065;
RA   Millman A., Bernheim A., Stokar-Avihail A., Fedorenko T., Voichek M.,
RA   Leavitt A., Oppenheimer-Shaanan Y., Sorek R.;
RT   "Bacterial Retrons Function In Anti-Phage Defense.";
RL   Cell 183:1551-1561(2020).
CC   -!- FUNCTION: Probable ATPase component of antiviral defense system retron
CC       Ec78, composed of a non-coding RNA (ncRNA), a reverse transcriptase
CC       (RT), this protein and a putative HNH endonuclease. Expression of
CC       retron Ec78 confers protection against bacteriophage T5. At
CC       multiplicity of infection (MOI) of 0.02 cultures slow growth when
CC       infected with T5 but do not collapse, at MOI 2 cultures enter growth
CC       stasis. {ECO:0000269|PubMed:33157039}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; JHRW01000018; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; U02551; -; NOT_ANNOTATED_CDS; Genomic_DNA.
PE   1: Evidence at protein level;
KW   Antiviral defense; ATP-binding; Nucleotide-binding.
FT   CHAIN           1..550
FT                   /note="Retron Ec78 probable ATPase"
FT                   /id="PRO_0000456024"
FT   MOTIF           93..100
FT                   /note="ATP-binding"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        16
FT                   /note="S -> Y (in Ref. 2; U02551)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        194..235
FT                   /note="TINLPTFALYNVERSQPFNRNTKDNAGRREERFDAYSQALGG -> QSTCRR
FT                   LLFTTLSDRNPLTATQKIMPDAEKSVLMPIVKLSV (in Ref. 2; U02551)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   550 AA;  62385 MW;  F9E73CFBC81BB7FA CRC64;
     MTKQYERKAK GGNLLSAFEL YQRNTDNMPG LGEMLVDEWF ETCRDYIQDG HVDESGTFRP
     DNAFYLRRLT LKDFRRFSLL EIKFEEDLTV IIGNNGKGKT SILYAIAKTL SWFVANILKE
     GGSGQRLSEL TDIKNDAENR YADVSSTFFF GKGLKSVPIR LSRSALGTAE RRDSEVKPAR
     DLADIWRVIN EAKTINLPTF ALYNVERSQP FNRNTKDNAG RREERFDAYS QALGGAGRFD
     HFVEWYIYLH KRTISDISSS IKELEQQVND LQRSVDGGMV SVKSLLEQMK LKLSEASERN
     DAAVSSKMVT ESVQKSIVEK SICSVVPSIS KIWVEMTTGS DLVKVTNDGH DVTIDQLSDG
     QRVFLSLVAD LARRMVMLNP LLENPLEGRG IVLIDEIELH LHPKWQQEVI LNLRSVFPNI
     QFIITTHSPI VLSTIEKRCI REFDPNDDGN QSFLDSPDMQ TKGSENAQIL EQVMNVHPTP
     PGIAESHWLG DFELLLLDNS GELDNQSQEL YDKIKTHFGI DSAELKKADS LIRINKMKNK
     INKIRAEKGK