PYRD1_DROME
ID PYRD1_DROME Reviewed; 472 AA.
AC Q9VIP2;
DT 05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1 {ECO:0000303|PubMed:30345904};
DE EC=1.8.1.-;
GN Name=Pyroxd1 {ECO:0000312|FlyBase:FBgn0032846};
GN ORFNames=CG10721 {ECO:0000312|FlyBase:FBgn0032846};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAY54852.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAY54852.1};
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Celniker S.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP DISRUPTION PHENOTYPE.
RX PubMed=30345904; DOI=10.1152/physiolgenomics.00036.2018;
RA Saha M., Reddy H.M., Salih M., Estrella E., Jones M.D., Mitsuhashi S.,
RA Cho K.A., Suzuki-Hatano S., Rizzo S.A., Hamad M.H., Mukhtar M.M.,
RA Hamed A.A., Elseed M.A., Lek M., Valkanas E., MacArthur D.G., Kunkel L.M.,
RA Pacak C.A., Draper I., Kang P.B.;
RT "The impact of PYROXD1 deficiency on cellular respiration and correlations
RT with genetic analyses of limb-girdle muscular dystrophy in Saudi Arabia and
RT Sudan.";
RL Physiol. Genomics 50:929-939(2018).
CC -!- FUNCTION: Probable oxidoreductase. {ECO:0000305}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000250|UniProtKB:O52582};
CC Note=Binds 1 FAD per subunit. {ECO:0000250|UniProtKB:O52582};
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in failure to
CC emerge from the pupal case and in lethality.
CC {ECO:0000269|PubMed:30345904}.
CC -!- SIMILARITY: Belongs to the class-I pyridine nucleotide-disulfide
CC oxidoreductase family. PYROXD1 subfamily. {ECO:0000305}.
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DR EMBL; AE014134; AAF53874.1; -; Genomic_DNA.
DR EMBL; AE014134; AGB93154.1; -; Genomic_DNA.
DR EMBL; BT022436; AAY54852.1; -; mRNA.
DR RefSeq; NP_001260619.1; NM_001273690.1.
DR RefSeq; NP_610023.1; NM_136179.3.
DR AlphaFoldDB; Q9VIP2; -.
DR SMR; Q9VIP2; -.
DR IntAct; Q9VIP2; 3.
DR STRING; 7227.FBpp0304005; -.
DR PaxDb; Q9VIP2; -.
DR DNASU; 35296; -.
DR EnsemblMetazoa; FBtr0081333; FBpp0080865; FBgn0032846.
DR EnsemblMetazoa; FBtr0331615; FBpp0304005; FBgn0032846.
DR GeneID; 35296; -.
DR KEGG; dme:Dmel_CG10721; -.
DR UCSC; CG10721-RA; d. melanogaster.
DR CTD; 79912; -.
DR FlyBase; FBgn0032846; Pyroxd1.
DR VEuPathDB; VectorBase:FBgn0032846; -.
DR eggNOG; KOG2755; Eukaryota.
DR GeneTree; ENSGT00390000014894; -.
DR HOGENOM; CLU_026335_0_0_1; -.
DR InParanoid; Q9VIP2; -.
DR OMA; MCENLIL; -.
DR OrthoDB; 1463391at2759; -.
DR PhylomeDB; Q9VIP2; -.
DR BioGRID-ORCS; 35296; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 35296; -.
DR PRO; PR:Q9VIP2; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032846; Expressed in embryonic/larval hemocyte (Drosophila) and 23 other tissues.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.390.30; -; 1.
DR Gene3D; 3.50.50.60; -; 3.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer_sf.
DR InterPro; IPR041575; Rubredoxin_C.
DR Pfam; PF07992; Pyr_redox_2; 2.
DR Pfam; PF18267; Rubredoxin_C; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
PE 2: Evidence at transcript level;
KW FAD; Flavoprotein; Oxidoreductase; Reference proteome.
FT CHAIN 1..472
FT /note="Pyridine nucleotide-disulfide oxidoreductase domain-
FT containing protein 1"
FT /id="PRO_0000445780"
SQ SEQUENCE 472 AA; 52351 MW; 46E5B911B3B4315B CRC64;
MSRKCEFLVV GGGIAGVSCA ESLAIYRPNA SILLLTESSI VKSVTNLVPV ARYLHKFDVR
EQDVSEMGAS FQTLVDRLDH INSREHCIRT KAGLEIKYRY LCLCTGGTPK LFSGKVVNPL
VIGIRDTDSV QLLQRKLATA KDVLILGNGG IASELAYELK DVNVHWVVKD SHISATFVDP
GAAEFFHIAM NECNAKDSSP VVAIKRMRYS EVLPKEQTNN HGAALGPDWH RSVDLSGARE
GEENRLPKIY YKSRISSVQD LADDAGAIVK LEHEDGSFQQ LTCDFIVSAT GVWPNTDYTC
DSPLQFSDDG GISVDEMMRT NLVDVFAAGD VCTANWPAAM HWFQMRLWTQ ARQMGSMAGR
SMAAASEGES VYQDFCFELF GHVTKLFGYP VVLLGRFNGQ DLGRDYEILV RCTRNKEYIK
FVLQNGRLRG AMLIGNTDLA ETCENLILNG IDLEPYGDDI LNPDIDIEDY FD
