ATP83_ECOLX
ID ATP83_ECOLX Reviewed; 542 AA.
AC Q47527;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 03-AUG-2022, sequence version 2.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Retron Ec83 probable ATPase {ECO:0000303|PubMed:1282191};
GN ORFNames=Ga0124318_11282;
OS Escherichia coli.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1] {ECO:0000312|EMBL:CAA78294.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PUTATIVE FUNCTION, AND DOMAIN.
RC STRAIN=Clinical strain 161;
RX PubMed=1282191; DOI=10.1111/j.1365-2958.1992.tb01788.x;
RA Lim D.;
RT "Structure and biosynthesis of unbranched multicopy single-stranded DNA by
RT reverse transcriptase in a clinical Escherichia coli isolate.";
RL Mol. Microbiol. 6:3531-3542(1992).
RN [2] {ECO:0000312|EMBL:CXYK01000012}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=05-2753;
RA Hur Y.J.;
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION IN ANTIVIRAL DEFENSE, AND IDENTIFICATION AS A RETRON.
RC STRAIN=05-2753;
RX PubMed=33157039; DOI=10.1016/j.cell.2020.09.065;
RA Millman A., Bernheim A., Stokar-Avihail A., Fedorenko T., Voichek M.,
RA Leavitt A., Oppenheimer-Shaanan Y., Sorek R.;
RT "Bacterial Retrons Function In Anti-Phage Defense.";
RL Cell 183:1551-1561(2020).
CC -!- FUNCTION: Probable ATPase component of antiviral defense system retron
CC Ec83, composed of a non-coding RNA (ncRNA), a reverse transcriptase
CC (RT), this protein and a putative HNH endonuclease. Expression of
CC retron Ec83 confers protection against bacteriophage T2, T4 and T6. At
CC multiplicity of infection (MOI) of 0.02 cultures slow growth when
CC infected with T4 but do not collapse, at MOI 2 cultures enter growth
CC stasis. {ECO:0000269|PubMed:33157039}.
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DR EMBL; Z12832; CAA78294.1; -; Genomic_DNA.
DR EMBL; CXYK01000012; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S28007; S28007.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF13304; AAA_21; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; ATP-binding; Nucleotide-binding.
FT CHAIN 1..542
FT /note="Retron Ec83 probable ATPase"
FT /id="PRO_0000456025"
FT MOTIF 92..99
FT /note="ATP-binding"
FT /evidence="ECO:0000305|PubMed:1282191"
FT CONFLICT 458..542
FT /note="GVRGAISSWVLENLFEVAQRPPEDKYTKLLQEYKNLVFSEKYASEDARKLGA
FT TLSQHFGPDDETLVELKLEIEKRIWEDDFEKDQ -> ELEEQYLLGYWRTCSKLLKGRQ
FT RISTQNSYRNIKI (in Ref. 1; CAA78294)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 542 AA; 61685 MW; 13EF9B0C1B1F765A CRC64;
MEQNLPSRIT KLIKKSESGD FASSYQLYKV FGSKEYGVEP DEKMSDYFKE LSAKQLEGGQ
LRVADIHLEN YKGFESLIMD FSMKKNSTIL VGNNGCGKST ILDAIQKGLT HLSSRLSTRS
HNGDGIEKHE LRKGQNYASI AINYDYMGIR FPMIIATTEP GYEDRAKSNY SGINELGSIF
KTAHSINPNV SFPLIAMYTV ERANDVSTRD IENSEEIKEA QIWDKFKAYN KSLTGKADFK
LFFRWFKELI EIENSDNADI TALRAEIRAK EKDLDNPLLK ALLAENKNSE TTKKLLEDHQ
NSLKVLKEKL NSYYSVNSKT LHTVEDAMYS FLPGFSNLKL QRAPLDLIVD KNNVSLSVLQ
LSQGEKTILA LIADIARRLT LLNPNSVNPL DGTGIVLIDE IDLHLHPSWQ QNIIPRLEKT
FKNIQFIVTT HSPQVCHTID SQNIWLLKNG QKFKAPKGVR GAISSWVLEN LFEVAQRPPE
DKYTKLLQEY KNLVFSEKYA SEDARKLGAT LSQHFGPDDE TLVELKLEIE KRIWEDDFEK
DQ