PYRF_CANAL
ID PYRF_CANAL Reviewed; 270 AA.
AC P13649; A0A1D8PJ56; Q5AJ76; Q9UVV6;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 4.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Orotidine 5'-phosphate decarboxylase;
DE EC=4.1.1.23;
DE AltName: Full=OMP decarboxylase;
DE Short=OMPDCase;
DE Short=OMPdecase;
DE AltName: Full=Uridine 5'-monophosphate synthase;
DE Short=UMP synthase;
GN Name=URA3; OrderedLocusNames=CAALFM_C301350CA;
GN ORFNames=CaO19.1716, CaO19.9284;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 10231 / CBS 6431 / DSM 1386 / NBRC 1594;
RX PubMed=2574635; DOI=10.1007/bf00391471;
RA Ernst J.F., Losberger C.;
RT "Sequence and transcript analysis of the C. albicans URA3 gene encoding
RT orotidine-5'-phosphate decarboxylase.";
RL Curr. Genet. 16:153-157(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1177;
RA Hoyer L.L.;
RT "Construction and evaluation of a Candida albicans gene expression
RT cassette.";
RL Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP;
CC Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10110};
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC UMP from orotate: step 2/2.
CC -!- SIMILARITY: Belongs to the OMP decarboxylase family. {ECO:0000305}.
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DR EMBL; X14198; CAA32410.1; -; Genomic_DNA.
DR EMBL; AF109400; AAF13298.1; -; Genomic_DNA.
DR EMBL; CP017625; AOW28178.1; -; Genomic_DNA.
DR PIR; A48331; DCCKA.
DR RefSeq; XP_721787.2; XM_716694.2.
DR AlphaFoldDB; P13649; -.
DR SMR; P13649; -.
DR STRING; 237561.P13649; -.
DR GeneID; 3636649; -.
DR KEGG; cal:CAALFM_C301350CA; -.
DR CGD; CAL0000191638; URA3.
DR VEuPathDB; FungiDB:C3_01350C_A; -.
DR eggNOG; KOG1377; Eukaryota.
DR HOGENOM; CLU_030821_0_0_1; -.
DR InParanoid; P13649; -.
DR OrthoDB; 1303452at2759; -.
DR UniPathway; UPA00070; UER00120.
DR PRO; PR:P13649; -.
DR Proteomes; UP000000559; Chromosome 3.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IBA:GO_Central.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IBA:GO_Central.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR014732; OMPdecase.
DR InterPro; IPR018089; OMPdecase_AS.
DR InterPro; IPR001754; OMPdeCOase_dom.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR PANTHER; PTHR32119:SF2; PTHR32119:SF2; 1.
DR Pfam; PF00215; OMPdecase; 1.
DR SMART; SM00934; OMPdecase; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
DR TIGRFAMs; TIGR01740; pyrF; 1.
DR PROSITE; PS00156; OMPDECASE; 1.
PE 3: Inferred from homology;
KW Decarboxylase; Lyase; Pyrimidine biosynthesis; Reference proteome.
FT CHAIN 1..270
FT /note="Orotidine 5'-phosphate decarboxylase"
FT /id="PRO_0000134646"
FT ACT_SITE 95
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10110"
FT BINDING 39
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 61..63
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 93..102
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 221
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 239
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 81
FT /note="G -> E (in Ref. 1; CAA32410 and 2; AAF13298)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="V -> I (in Ref. 1; CAA32410 and 2; AAF13298)"
FT /evidence="ECO:0000305"
FT CONFLICT 257
FT /note="D -> N (in Ref. 1; CAA32410)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 270 AA; 29893 MW; 1B4FC3B48EBB727D CRC64;
MTVNTKTYSE RAETHASPVA QRLFRLMESK KTNLCASIDV DTTKEFLELI DKLGPYVCLI
KTHIDIINDF SYESTIEPLL GLSRKHQFMI FEDRKFADIG NTVKKQYIGG VYKISSWADI
TNAHGVTGNG VVEGLKQGAK ETTTNQEPRG LLMLAELSSV GSLAYGEYSQ KTVEIAKSDK
EFVVGFIAQR DMGGQEEGFD WLIMTPGVGL DDKGDGLGQQ YRTVDEVVST GTDIIIVGRG
LFGKGRDPDI EGKRYRDAGW NAYLKKTGQL
