PYRF_COCIM
ID PYRF_COCIM Reviewed; 274 AA.
AC Q1E9A1; J3KI15; Q9C0T0; Q9C144; Q9C145;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Orotidine 5'-phosphate decarboxylase;
DE EC=4.1.1.23;
DE AltName: Full=OMP decarboxylase;
DE Short=OMPDCase;
DE Short=OMPdecase;
DE AltName: Full=Uridine 5'-monophosphate synthase;
DE Short=UMP synthase;
GN Name=URA3; ORFNames=CIMG_00862;
OS Coccidioides immitis (strain RS) (Valley fever fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Onygenaceae; Coccidioides.
OX NCBI_TaxID=246410;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RS;
RX PubMed=19717792; DOI=10.1101/gr.087551.108;
RA Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA Henn M.R., Birren B.W., Taylor J.W.;
RT "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT and their relatives.";
RL Genome Res. 19:1722-1731(2009).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=RS;
RX PubMed=20516208; DOI=10.1101/gr.103911.109;
RA Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J.,
RA Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D.,
RA Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A.,
RA FitzGerald M., Lui A., Macdonald J.P., Priest M., Orbach M.J.,
RA Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R.,
RA Taylor J.W., Rounsley S.D.;
RT "Population genomic sequencing of Coccidioides fungi reveals recent
RT hybridization and transposon control.";
RL Genome Res. 20:938-946(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 70-201, AND VARIANTS SER-75 AND
RP VAL-76.
RC STRAIN=RMSCC 1695 / CA3, RMSCC 2019 / CA4, and RMSCC 2267 / CA1;
RX PubMed=9144263; DOI=10.1073/pnas.94.10.5478;
RA Koufopanou V., Burt A., Taylor J.W.;
RT "Concordance of gene genealogies reveals reproductive isolation in the
RT pathogenic fungus Coccidioides immitis.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:5478-5482(1997).
RN [4]
RP ERRATUM OF PUBMED:9144263.
RA Koufopanou V., Burt A., Taylor J.W.;
RL Proc. Natl. Acad. Sci. U.S.A. 95:8414-8414(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP;
CC Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10110};
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC UMP from orotate: step 2/2.
CC -!- SIMILARITY: Belongs to the OMP decarboxylase family. {ECO:0000305}.
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DR EMBL; GG704911; EAS35508.1; -; Genomic_DNA.
DR EMBL; AJ292103; CAC35047.1; -; Genomic_DNA.
DR EMBL; AJ292104; CAC35048.1; -; Genomic_DNA.
DR EMBL; AJ292105; CAC35049.1; -; Genomic_DNA.
DR RefSeq; XP_001247091.1; XM_001247090.2.
DR AlphaFoldDB; Q1E9A1; -.
DR SMR; Q1E9A1; -.
DR STRING; 246410.Q1E9A1; -.
DR EnsemblFungi; EAS35508; EAS35508; CIMG_00862.
DR GeneID; 4567662; -.
DR KEGG; cim:CIMG_00862; -.
DR VEuPathDB; FungiDB:CIMG_00862; -.
DR InParanoid; Q1E9A1; -.
DR OMA; KNFVMGF; -.
DR OrthoDB; 1303452at2759; -.
DR UniPathway; UPA00070; UER00120.
DR Proteomes; UP000001261; Unassembled WGS sequence.
DR GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR014732; OMPdecase.
DR InterPro; IPR018089; OMPdecase_AS.
DR InterPro; IPR001754; OMPdeCOase_dom.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR PANTHER; PTHR32119:SF2; PTHR32119:SF2; 1.
DR Pfam; PF00215; OMPdecase; 1.
DR SMART; SM00934; OMPdecase; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
DR TIGRFAMs; TIGR01740; pyrF; 1.
DR PROSITE; PS00156; OMPDECASE; 1.
PE 3: Inferred from homology;
KW Decarboxylase; Lyase; Pyrimidine biosynthesis; Reference proteome.
FT CHAIN 1..274
FT /note="Orotidine 5'-phosphate decarboxylase"
FT /id="PRO_0000252292"
FT ACT_SITE 95
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10110"
FT BINDING 40
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 62..64
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 93..102
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 227
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 245
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT VARIANT 75
FT /note="T -> S (in strain: RMSCC 1695 / CA3)"
FT /evidence="ECO:0000269|PubMed:9144263"
FT VARIANT 76
FT /note="I -> V (in strain: RMSCC 1695 / CA3 and RMSCC 2267 /
FT CA1)"
FT /evidence="ECO:0000269|PubMed:9144263"
SQ SEQUENCE 274 AA; 29606 MW; 9076091670B57E43 CRC64;
MASKSQVPYE DRARDHPNPL ARRLFQIATE KQSNVAVSAD VTTTKELLDL ADRLGPYMVV
LKTHIDILAD FSAETITGLQ SLSQKHNFLI FEDRKFVDIG NTVQKQYHGG ALHISEWAHI
VNATVLPGPG IIDALAQVAS APDFPHASDR GLLILATMTS KGSLATGQYT ELSVELARKY
KGFVLGFVAS RSLEGVETAG KADDEDFVLF TTGVNLASKG DALGQQYQTP ESAIGGGADF
IISGRGIYAA PDPVDAARRY QKAGWDAYLK RVGR
