位置:首页 > 蛋白库 > PYRF_LACLA
PYRF_LACLA
ID   PYRF_LACLA              Reviewed;         237 AA.
AC   Q9CFW9; Q9L9C5;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Orotidine 5'-phosphate decarboxylase {ECO:0000255|HAMAP-Rule:MF_01200};
DE            EC=4.1.1.23 {ECO:0000255|HAMAP-Rule:MF_01200};
DE   AltName: Full=OMP decarboxylase {ECO:0000255|HAMAP-Rule:MF_01200};
DE            Short=OMPDCase {ECO:0000255|HAMAP-Rule:MF_01200};
DE            Short=OMPdecase {ECO:0000255|HAMAP-Rule:MF_01200};
GN   Name=pyrF {ECO:0000255|HAMAP-Rule:MF_01200}; OrderedLocusNames=LL1345;
GN   ORFNames=L181692;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CHCC377;
RX   PubMed=10742196; DOI=10.1128/aem.66.4.1253-1258.2000;
RA   Soerensen K.I., Larsen R., Kibenich A., Junge M.P., Johansen E.;
RT   "A food-grade cloning system for industrial strains of Lactococcus
RT   lactis.";
RL   Appl. Environ. Microbiol. 66:1253-1258(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- FUNCTION: Catalyzes the decarboxylation of orotidine 5'-monophosphate
CC       (OMP) to uridine 5'-monophosphate (UMP). {ECO:0000255|HAMAP-
CC       Rule:MF_01200}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP;
CC         Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01200};
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC       UMP from orotate: step 2/2. {ECO:0000255|HAMAP-Rule:MF_01200}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01200}.
CC   -!- SIMILARITY: Belongs to the OMP decarboxylase family. Type 1 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01200}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF174425; AAF63958.1; -; Genomic_DNA.
DR   EMBL; AE005176; AAK05443.1; -; Genomic_DNA.
DR   PIR; A86793; A86793.
DR   RefSeq; NP_267501.1; NC_002662.1.
DR   RefSeq; WP_003131093.1; NC_002662.1.
DR   AlphaFoldDB; Q9CFW9; -.
DR   SMR; Q9CFW9; -.
DR   STRING; 272623.L181692; -.
DR   PaxDb; Q9CFW9; -.
DR   EnsemblBacteria; AAK05443; AAK05443; L181692.
DR   KEGG; lla:L181692; -.
DR   PATRIC; fig|272623.7.peg.1451; -.
DR   eggNOG; COG0284; Bacteria.
DR   HOGENOM; CLU_067069_1_1_9; -.
DR   OMA; NFKIFLD; -.
DR   UniPathway; UPA00070; UER00120.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.20.20.70; -; 1.
DR   HAMAP; MF_01200_B; OMPdecase_type1_B; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR014732; OMPdecase.
DR   InterPro; IPR018089; OMPdecase_AS.
DR   InterPro; IPR001754; OMPdeCOase_dom.
DR   InterPro; IPR011060; RibuloseP-bd_barrel.
DR   PANTHER; PTHR32119:SF2; PTHR32119:SF2; 1.
DR   Pfam; PF00215; OMPdecase; 1.
DR   SMART; SM00934; OMPdecase; 1.
DR   SUPFAM; SSF51366; SSF51366; 1.
DR   TIGRFAMs; TIGR01740; pyrF; 1.
DR   PROSITE; PS00156; OMPDECASE; 1.
PE   3: Inferred from homology;
KW   Decarboxylase; Lyase; Pyrimidine biosynthesis; Reference proteome.
FT   CHAIN           1..237
FT                   /note="Orotidine 5'-phosphate decarboxylase"
FT                   /id="PRO_0000134549"
FT   ACT_SITE        63
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01200"
FT   BINDING         11
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01200"
FT   BINDING         34
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01200"
FT   BINDING         61..70
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01200"
FT   BINDING         124
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01200"
FT   BINDING         186
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01200"
FT   BINDING         195
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01200"
FT   BINDING         215
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01200"
FT   BINDING         216
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01200"
FT   CONFLICT        76
FT                   /note="H -> R (in Ref. 1; AAF63958)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        83
FT                   /note="V -> L (in Ref. 1; AAF63958)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        129
FT                   /note="E -> K (in Ref. 1; AAF63958)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        237
FT                   /note="R -> Q (in Ref. 1; AAF63958)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   237 AA;  26106 MW;  BBAA395B4DE31A9B CRC64;
     MQENRPVIAL DFPEFSDVKD FLEKFDPSEQ LYIKLGMELF YTAGPQVVYY VKSLGHSVFL
     DLKLHDIPNT VESSMHVLAR LGVDMVNVHA AGGVEMMVAA KRGLEAGTPV GRQRPKLIAV
     TQLTSTSEEI MQNDQKIMTS LEESVINYAQ KTAQAGLDGV VCSAHEVEKI KAATSKEFIC
     LTPGIRPEGA SKGDQKRVMT PKEARTIGSD YIVVGRPITQ AKDPVASYHA IKAEWNR
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024