PYRF_NEUCR
ID PYRF_NEUCR Reviewed; 397 AA.
AC P05035; Q7RV44;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 13-AUG-1987, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Orotidine 5'-phosphate decarboxylase;
DE EC=4.1.1.23;
DE AltName: Full=OMP decarboxylase;
DE Short=OMPDCase;
DE Short=OMPdecase;
DE AltName: Full=Uridine 5'-monophosphate synthase;
DE Short=UMP synthase;
GN Name=pyr-4; ORFNames=B23H20.130, NCU03488;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3019837; DOI=10.1016/0378-1119(86)90007-7;
RA Newbury S.F., Glazebrook J.A., Radford A.;
RT "Sequence analysis of the pyr-4 (orotidine 5'-P decarboxylase) gene of
RT Neurospora crassa.";
RL Gene 43:51-58(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2959843; DOI=10.1007/bf00329672;
RA Glazebrook J.A., Mitchell K., Radford A.;
RT "Molecular genetic analysis of the pyr-4 gene of Neurospora crassa.";
RL Mol. Gen. Genet. 209:399-402(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP;
CC Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10110};
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC UMP from orotate: step 2/2.
CC -!- SIMILARITY: Belongs to the OMP decarboxylase family. {ECO:0000305}.
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DR EMBL; M13448; AAA33611.1; -; Genomic_DNA.
DR EMBL; X05993; CAA29411.1; -; Genomic_DNA.
DR EMBL; AL669988; CAD21085.1; -; Genomic_DNA.
DR EMBL; CM002237; EAA26639.1; -; Genomic_DNA.
DR PIR; A24398; DCNCOP.
DR RefSeq; XP_955875.1; XM_950782.3.
DR AlphaFoldDB; P05035; -.
DR SMR; P05035; -.
DR STRING; 5141.EFNCRP00000002693; -.
DR EnsemblFungi; EAA26639; EAA26639; NCU03488.
DR GeneID; 3872022; -.
DR KEGG; ncr:NCU03488; -.
DR VEuPathDB; FungiDB:NCU03488; -.
DR HOGENOM; CLU_030821_1_1_1; -.
DR InParanoid; P05035; -.
DR OMA; KNFVMGF; -.
DR UniPathway; UPA00070; UER00120.
DR Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IBA:GO_Central.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IBA:GO_Central.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR014732; OMPdecase.
DR InterPro; IPR018089; OMPdecase_AS.
DR InterPro; IPR001754; OMPdeCOase_dom.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR PANTHER; PTHR32119:SF2; PTHR32119:SF2; 1.
DR Pfam; PF00215; OMPdecase; 1.
DR SMART; SM00934; OMPdecase; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
DR PROSITE; PS00156; OMPDECASE; 1.
PE 3: Inferred from homology;
KW Decarboxylase; Lyase; Pyrimidine biosynthesis; Reference proteome.
FT CHAIN 1..397
FT /note="Orotidine 5'-phosphate decarboxylase"
FT /id="PRO_0000134665"
FT ACT_SITE 107
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10110"
FT BINDING 48
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 70..72
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 105..114
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 347
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 366
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 397 AA; 43903 MW; C9A39CDA33A3FCD9 CRC64;
MSTSQETQPH WSLKQSFAER VESSTHPLTS YLFRLMEVKQ SNLCLSADVE HARDLLALAD
KVGPSIVVLK THYDLITGWD YHPHTGTGAK LAALARKHGF LIFEDRKFVD IGSTVQKQYT
AGTARIVEWA HITNADIHAG EAMVSAMAQA AQKWRERIPY EVKTSVSVGT PVADQFADEE
AEDQVEELRK VVTRETSTTT KDTDGRKSSI VSITTVTQTY EPADSPRLVK TISEDDEMVF
PGIEEAPLDR GLLILAQMSS KGCLMDGKYT WECVKAARKN KGFVMGYVAQ QNLNGITKEA
LAPSYEDGES TTEEEAQADN FIHMTPGCKL PPPGEEAPQG DGLGQQYNTP DNLVNIKGTD
IAIVGRGIIT AADPPAEAER YRRKAWKAYQ DRRERLA
