PYRF_PICAN
ID PYRF_PICAN Reviewed; 263 AA.
AC Q06375;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Orotidine 5'-phosphate decarboxylase;
DE EC=4.1.1.23;
DE AltName: Full=OMP decarboxylase;
DE Short=OMPDCase;
DE Short=OMPdecase;
DE AltName: Full=Uridine 5'-monophosphate synthase;
DE Short=UMP synthase;
GN Name=URA3;
OS Pichia angusta (Yeast) (Hansenula polymorpha).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Pichiaceae; Ogataea.
OX NCBI_TaxID=870730;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL
RC Y-5445;
RX PubMed=7764392; DOI=10.1007/bf00170393;
RA Merckelbach A., Goedecke S., Janowicz Z.A., Hollenberg C.P.;
RT "Cloning and sequencing of the ura3 locus of the methylotrophic yeast
RT Hansenula polymorpha and its use for the generation of a deletion by gene
RT replacement.";
RL Appl. Microbiol. Biotechnol. 40:361-364(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP;
CC Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10110};
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC UMP from orotate: step 2/2.
CC -!- SIMILARITY: Belongs to the OMP decarboxylase family. {ECO:0000305}.
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DR EMBL; X69461; CAA49221.1; -; Genomic_DNA.
DR PIR; S31323; S31323.
DR AlphaFoldDB; Q06375; -.
DR SMR; Q06375; -.
DR PRIDE; Q06375; -.
DR UniPathway; UPA00070; UER00120.
DR GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR014732; OMPdecase.
DR InterPro; IPR018089; OMPdecase_AS.
DR InterPro; IPR001754; OMPdeCOase_dom.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR PANTHER; PTHR32119:SF2; PTHR32119:SF2; 1.
DR Pfam; PF00215; OMPdecase; 1.
DR SMART; SM00934; OMPdecase; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
DR TIGRFAMs; TIGR01740; pyrF; 1.
DR PROSITE; PS00156; OMPDECASE; 1.
PE 3: Inferred from homology;
KW Decarboxylase; Lyase; Pyrimidine biosynthesis.
FT CHAIN 1..263
FT /note="Orotidine 5'-phosphate decarboxylase"
FT /id="PRO_0000134672"
FT ACT_SITE 92
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10110"
FT BINDING 36
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 58..60
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 90..99
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 216
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 234
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 263 AA; 29275 MW; 0934EF673B03A820 CRC64;
MYKSYGERAK SHPSKVASRL LNLMESKQTN LCASVDVTKT QELLELLDKL GPYICLVKTH
IDIVEDFSYE HTILPLQGLA KKHNFMIFED RKFADIGNTV KLQYKGGIYR TSKWADITNA
HGVTGAGIVE GLKQAAEEST DEPRGLLMLA ELSSKGSLAT GEYTQKTVEI AKSDKEFVIG
FIAQRDMGGR EEGFDWLIMT PGVGLDDKGD SLGQQYRTVD EVMQTGTDVI IVGRGLFGKG
RDPEVEGKRY RNAGWEAYKR RIA
