PYRF_SCHPO
ID PYRF_SCHPO Reviewed; 264 AA.
AC P14965;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Orotidine 5'-phosphate decarboxylase;
DE EC=4.1.1.23;
DE AltName: Full=OMP decarboxylase;
DE Short=OMPDCase;
DE Short=OMPdecase;
DE AltName: Full=Uridine 5'-monophosphate synthase;
DE Short=UMP synthase;
GN Name=ura4; ORFNames=SPCC330.05c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3241624; DOI=10.1007/bf00331307;
RA Grimm C., Kohli J., Murray J., Maundrell K.;
RT "Genetic engineering of Schizosaccharomyces pombe: a system for gene
RT disruption and replacement using the ura4 gene as a selectable marker.";
RL Mol. Gen. Genet. 215:81-86(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP;
CC Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10110};
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC UMP from orotate: step 2/2.
CC -!- SIMILARITY: Belongs to the OMP decarboxylase family. {ECO:0000305}.
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DR EMBL; X13976; CAA32157.1; -; Genomic_DNA.
DR EMBL; CU329672; CAA20910.1; -; Genomic_DNA.
DR PIR; S08503; S08503.
DR RefSeq; NP_587705.1; NM_001022700.2.
DR AlphaFoldDB; P14965; -.
DR SMR; P14965; -.
DR BioGRID; 275351; 14.
DR STRING; 4896.SPCC330.05c.1; -.
DR iPTMnet; P14965; -.
DR PaxDb; P14965; -.
DR PRIDE; P14965; -.
DR EnsemblFungi; SPCC330.05c.1; SPCC330.05c.1:pep; SPCC330.05c.
DR GeneID; 2538768; -.
DR KEGG; spo:SPCC330.05c; -.
DR PomBase; SPCC330.05c; ura4.
DR VEuPathDB; FungiDB:SPCC330.05c; -.
DR eggNOG; KOG1377; Eukaryota.
DR HOGENOM; CLU_030821_0_0_1; -.
DR InParanoid; P14965; -.
DR OMA; KNFVMGF; -.
DR PhylomeDB; P14965; -.
DR UniPathway; UPA00070; UER00120.
DR PRO; PR:P14965; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0140453; C:protein aggregate center; IDA:PomBase.
DR GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IMP:PomBase.
DR GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IGI:PomBase.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IMP:PomBase.
DR Gene3D; 3.20.20.70; -; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR014732; OMPdecase.
DR InterPro; IPR018089; OMPdecase_AS.
DR InterPro; IPR001754; OMPdeCOase_dom.
DR InterPro; IPR011060; RibuloseP-bd_barrel.
DR PANTHER; PTHR32119:SF2; PTHR32119:SF2; 1.
DR Pfam; PF00215; OMPdecase; 1.
DR SMART; SM00934; OMPdecase; 1.
DR SUPFAM; SSF51366; SSF51366; 1.
DR TIGRFAMs; TIGR01740; pyrF; 1.
DR PROSITE; PS00156; OMPDECASE; 1.
PE 3: Inferred from homology;
KW Decarboxylase; Lyase; Pyrimidine biosynthesis; Reference proteome.
FT CHAIN 1..264
FT /note="Orotidine 5'-phosphate decarboxylase"
FT /id="PRO_0000134683"
FT ACT_SITE 95
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10110"
FT BINDING 40
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 62..64
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 93..102
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 214
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 233
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 264 AA; 29362 MW; C9B78224E1EE1299 CRC64;
MDARVFQSYS ARAEGMKNPI AKELLALMEE KQSNLSVAVD LTKKSEILEL VDKIGPYVCV
IKTHIDVVED FDQDMVEKLV ALGKKHRFLI FEDRKFADIG NTVKLQYASG VYKIASWAHI
TNCHTVPGEG IIQGLKEVGL PLGRGLLLLA EMSSKGSLAT GSYTEKTLEW FEKHTDFCFG
FIAGRRFPNL QSDYITMSPG IGLDVKGDGL GQQYRTPEEV IVNCGSDIII VGRGVYGAGR
NPVVEAKRYR EAGWKAYQQR LSQH
