ID   ATP95_VIBCE             Reviewed;         514 AA.
AC   P0DV98;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Retron Vc95 probable ATPase {ECO:0000303|PubMed:10564503};
DE            Short=ORF540 {ECO:0000303|PubMed:10564503};
GN   ORFNames=Ga0059260_01848, KV36_09040.1 {ECO:0000303|Ref.1};
OS   Vibrio cholerae serotype O1 biovar El Tor.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=686;
RN   [1] {ECO:0000312|EMBL:JRBD01000006}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MAK676;
RA   Kritzky A., Cheldyshova N.B., Kulshan T.A., Krasnov Y.M., Cherkasov A.V.,
RA   Smirnova N.I.;
RT   "Vibrio cholerae O1 biovar El Tor MAK676 whole genome shotgun sequence.";
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   IDENTIFICATION.
RC   STRAIN=O139;
RX   PubMed=10564503; DOI=10.1046/j.1365-2958.1999.01531.x;
RA   Shimamoto T., Kobayashi M., Tsuchiya T., Shinoda S., Kawakami H.,
RA   Inouye S., Inouye M.;
RT   "A retroelement in Vibrio cholerae.";
RL   Mol. Microbiol. 34:631-632(1999).
RN   [3]
RP   FUNCTION IN ANTIVIRAL DEFENSE, AND IDENTIFICATION AS A RETRON.
RC   STRAIN=MAK676;
RX   PubMed=33157039; DOI=10.1016/j.cell.2020.09.065;
RA   Millman A., Bernheim A., Stokar-Avihail A., Fedorenko T., Voichek M.,
RA   Leavitt A., Oppenheimer-Shaanan Y., Sorek R.;
RT   "Bacterial Retrons Function In Anti-Phage Defense.";
RL   Cell 183:1551-1561(2020).
CC   -!- FUNCTION: Probable ATPase component of antiviral defense system retron
CC       Vc95, composed of a non-coding RNA (ncRNA), a reverse transcriptase
CC       (RT), this protein and a putative HNH endonuclease. Expression of
CC       retron Vc95 confers protection against bacteriophages T2, T4 and T6. At
CC       multiplicity of infection (MOI) of 0.02 cultures slow growth when
CC       infected with T4 but do not collapse, at MOI 2 cultures enter growth
CC       stasis. {ECO:0000269|PubMed:33157039}.
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DR   EMBL; JRBD01000006; -; NOT_ANNOTATED_CDS; Genomic_DNA.
PE   1: Evidence at protein level;
KW   Antiviral defense; ATP-binding; Nucleotide-binding.
FT   CHAIN           1..514
FT                   /note="Retron Vc95 probable ATPase"
FT                   /id="PRO_0000456026"
FT   MOTIF           92..99
FT                   /note="ATP-binding"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   514 AA;  59688 MW;  724061B8ADE31E98 CRC64;
     MNEPTRIPKY VKDKIRQAKQ GDLYSSFTVS EYYYDGEVLE RDIEQSELYL RNVSRIINNA
     KIRLRNISLY DYKKFSKLKF TSSEKNTTII IGNNGSGKST ILESISKCLQ FLSDNIRIQN
     NNNYKFQDSE INIHSISGQT IVRCILEIEN DFSFSCSLTK NRENISRKVS SELEEFKALA
     RMYQRSNELD NNTLSYPLLA YYPVERSVTL KRDDAVKYYE RKKAKYSDKS EGLKNAFDGT
     SNFNDFFSWY KEIDDIINEF KANDSITKEE IEYLLSKTDN KEKIGSLISQ LLEKKNNYNN
     NEDREFLIRQ QKVIQESIKT FVSDIDQVKI SRTPHLDMTV IKNGSEISIF NLSQGEKTLI
     ALVSDIARRL VILNPSLENP LNGYGIVLID EIDLHLHPKW QQTIVQKLEN TFPNIQFILS
     THSPLVLTTV TSEQIKIINE LDYRFKLLSP TSNPFGKNAS DALAIMETSE SPLVHSEEIL
     ALIKKYESLV KRGQEDCRKT KEIKKTHRKH WIYI