ID   PYRF_YARLI              Reviewed;         286 AA.
AC   Q12724; Q6C4H6; Q9C1U4;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Orotidine 5'-phosphate decarboxylase;
DE            EC=4.1.1.23;
DE   AltName: Full=OMP decarboxylase;
DE            Short=OMPDCase;
DE            Short=OMPdecase;
DE   AltName: Full=Uridine 5'-monophosphate synthase;
DE            Short=UMP synthase;
GN   Name=URA3; OrderedLocusNames=YALI0E26741g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 20460 / W29 / CBS 7504 / IFP29;
RX   PubMed=11489863; DOI=10.1128/jb.183.17.5102-5109.2001;
RA   Mauersberger S., Wang H., Gaillardin C., Barth G., Nicaud J.-M.;
RT   "Insertional mutagenesis in the n-alkane-assimilating yeast Yarrowia
RT   lipolytica: generation of tagged mutations in genes involved in hydrophobic
RT   substrate utilization.";
RL   J. Bacteriol. 183:5102-5109(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Strick C.A., James L.C., O'Donnell M.M., Elsenboss L.A.;
RL   Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-7 AND 250-284.
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP;
CC         Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10110};
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC       UMP from orotate: step 2/2.
CC   -!- SIMILARITY: Belongs to the OMP decarboxylase family. {ECO:0000305}.
CC   -!- CAUTION: Strain CLIB 122 / E 150 has a defective URA3 sequence (ura3-
CC       302) which is disrupted (positions 8 to 249) by S.cerevisiae SUC2.
CC       {ECO:0000305}.
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DR   EMBL; AJ306421; CAC32856.1; -; Genomic_DNA.
DR   EMBL; U40564; AAA85392.1; -; Genomic_DNA.
DR   EMBL; CR382131; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; Q12724; -.
DR   SMR; Q12724; -.
DR   STRING; 284591.Q12724; -.
DR   InParanoid; Q12724; -.
DR   UniPathway; UPA00070; UER00120.
DR   Proteomes; UP000001300; Chromosome E.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IBA:GO_Central.
DR   GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IBA:GO_Central.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR014732; OMPdecase.
DR   InterPro; IPR018089; OMPdecase_AS.
DR   InterPro; IPR001754; OMPdeCOase_dom.
DR   InterPro; IPR011060; RibuloseP-bd_barrel.
DR   PANTHER; PTHR32119:SF2; PTHR32119:SF2; 1.
DR   Pfam; PF00215; OMPdecase; 1.
DR   SMART; SM00934; OMPdecase; 1.
DR   SUPFAM; SSF51366; SSF51366; 1.
DR   TIGRFAMs; TIGR01740; pyrF; 2.
DR   PROSITE; PS00156; OMPDECASE; 1.
PE   3: Inferred from homology;
KW   Decarboxylase; Lyase; Pyrimidine biosynthesis; Reference proteome.
FT   CHAIN           1..286
FT                   /note="Orotidine 5'-phosphate decarboxylase"
FT                   /id="PRO_0000134691"
FT   ACT_SITE        91
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10110"
FT   BINDING         35
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         57..59
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         89..98
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         239
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         257
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        104..108
FT                   /note="KNGVY -> RCH (in Ref. 1; CAC32856)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   286 AA;  31630 MW;  7E71BFE84C61F216 CRC64;
     MPSYEARANV HKSAFAARVL KLVAAKKTNL CASLDVTTTK ELIELADKVG PYVCMIKTHI
     DIIDDFTYAG TVLPLKELAL KHGFFLFEDR KFADIGNTVK HQYKNGVYRI AEWSDITNAH
     GVPGTGIIAG LRAGAEETVS EQKKEDVSDY ENSQYKEFLV PSPNEKLARG LLMLAELSCK
     GSLATGEYSK QTIELARSDP EFVVGFIAQN RPKGDSEDWL ILTPGVGLDD KGDALGQQYR
     TVEDVMSTGT DIIIVGRGLY GQNRDPIEEA KRYQKAGWEA YQKINC