ID   PYRKH_THEMA             Reviewed;         282 AA.
AC   Q9X1X4;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit homolog;
DE   AltName: Full=Dihydroorotate oxidase B, electron transfer subunit homolog;
GN   OrderedLocusNames=TM_1639;
OS   Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS   / MSB8).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=243274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=10360571; DOI=10.1038/20601;
RA   Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA   Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA   Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA   Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA   Smith H.O., Venter J.C., Fraser C.M.;
RT   "Evidence for lateral gene transfer between Archaea and Bacteria from
RT   genome sequence of Thermotoga maritima.";
RL   Nature 399:323-329(1999).
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Evidence={ECO:0000250};
CC       Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC       Note=Binds 1 FAD per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the PyrK family. {ECO:0000305}.
CC   -!- CAUTION: Lacks the first cysteine that binds the 2Fe-2S complex.
CC       {ECO:0000305}.
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DR   EMBL; AE000512; AAD36706.1; -; Genomic_DNA.
DR   PIR; G72230; G72230.
DR   RefSeq; NP_229439.1; NC_000853.1.
DR   PDB; 4YLF; X-ray; 2.30 A; A/C=1-276.
DR   PDB; 4YRY; X-ray; 2.40 A; A/C=1-276.
DR   PDBsum; 4YLF; -.
DR   PDBsum; 4YRY; -.
DR   AlphaFoldDB; Q9X1X4; -.
DR   SMR; Q9X1X4; -.
DR   STRING; 243274.THEMA_06050; -.
DR   EnsemblBacteria; AAD36706; AAD36706; TM_1639.
DR   KEGG; tma:TM1639; -.
DR   PATRIC; fig|243274.5.peg.1658; -.
DR   eggNOG; COG0543; Bacteria.
DR   InParanoid; Q9X1X4; -.
DR   OMA; AGQFIIL; -.
DR   BRENDA; 1.6.1.4; 6331.
DR   Proteomes; UP000008183; Chromosome.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.80; -; 1.
DR   InterPro; IPR008333; Cbr1-like_FAD-bd_dom.
DR   InterPro; IPR012165; Cyt_c3_hydrogenase_gsu.
DR   InterPro; IPR019480; Dihydroorotate_DH_Fe-S-bd.
DR   InterPro; IPR017927; FAD-bd_FR_type.
DR   InterPro; IPR039261; FNR_nucleotide-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR   InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR   Pfam; PF10418; DHODB_Fe-S_bind; 1.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PIRSF; PIRSF006816; Cyc3_hyd_g; 1.
DR   SUPFAM; SSF52343; SSF52343; 1.
DR   SUPFAM; SSF63380; SSF63380; 1.
DR   PROSITE; PS51384; FAD_FR; 1.
PE   1: Evidence at protein level;
KW   2Fe-2S; 3D-structure; Electron transport; FAD; Flavoprotein; Iron;
KW   Iron-sulfur; Metal-binding; Reference proteome; Transport.
FT   CHAIN           1..282
FT                   /note="Dihydroorotate dehydrogenase B (NAD(+)), electron
FT                   transfer subunit homolog"
FT                   /id="PRO_0000148372"
FT   DOMAIN          2..100
FT                   /note="FAD-binding FR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00716"
FT   BINDING         225
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000250"
FT   BINDING         228
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000250"
FT   BINDING         240
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000250"
FT   STRAND          6..14
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          16..25
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   HELIX           27..32
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          38..44
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          50..58
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   TURN            59..62
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          63..69
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   HELIX           73..80
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          88..95
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          104..111
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   HELIX           112..115
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   TURN            116..118
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   HELIX           119..126
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   TURN            127..129
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          131..137
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          139..144
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   HELIX           149..154
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          155..169
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   HELIX           171..174
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   TURN            175..181
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          187..192
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   HELIX           193..201
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   TURN            202..205
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          210..213
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          219..225
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          229..232
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          235..238
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   TURN            239..241
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   STRAND          244..247
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   TURN            248..250
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   HELIX           253..260
FT                   /evidence="ECO:0007829|PDB:4YLF"
FT   HELIX           267..273
FT                   /evidence="ECO:0007829|PDB:4YLF"
SQ   SEQUENCE   282 AA;  31091 MW;  BEA9816C469D2AF1 CRC64;
     MGGTALNEIV KKVKIAEDVF DFWIHSPSVS KEARPGQFVV IRLHEKGERI PLTVADTKPE
     EGLFRMVVKV VGKTTHELSL KKEGDTILDV VGPLGNPSEI ENYGNVLLVG GGVGIATLYP
     IAKALKEAGN NITTVLGART KDYLIMVDEF KEISDVLLVT DDGSAGMKGV VTDAMDKLFR
     ERKFDICWAV GPTIMMKFCT LKAREFGVPI WVSLNPIMVD GTGMCGACRV TVSGQIKFAC
     VDGPEFRGEE VDWDELLKRL AQYREQEKIS YERFLKTAGE SE