PYRP_BACSU
ID PYRP_BACSU Reviewed; 435 AA.
AC P39766; P25982;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Uracil permease;
DE AltName: Full=Uracil transporter;
GN Name=pyrP; OrderedLocusNames=BSU15480;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1709162; DOI=10.1016/s0021-9258(18)31559-x;
RA Quinn C.L., Stephenson B.T., Switzer R.L.;
RT "Functional organization and nucleotide sequence of the Bacillus subtilis
RT pyrimidine biosynthetic operon.";
RL J. Biol. Chem. 266:9113-9127(1991).
RN [2]
RP SEQUENCE REVISION, AND CHARACTERIZATION.
RX PubMed=8206849; DOI=10.1128/jb.176.12.3708-3722.1994;
RA Turner R.J., Lu Y., Switzer R.L.;
RT "Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene
RT cluster by an autogenous transcriptional attenuation mechanism.";
RL J. Bacteriol. 176:3708-3722(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP SEQUENCE REVISION TO 231-260 AND 271-272.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
CC -!- FUNCTION: Transport of uracil in the cell.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. {ECO:0000305}.
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DR EMBL; M59757; AAA21266.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13422.2; -; Genomic_DNA.
DR PIR; A38984; A57986.
DR RefSeq; NP_389431.2; NC_000964.3.
DR RefSeq; WP_003221479.1; NZ_JNCM01000035.1.
DR RefSeq; WP_009967203.1; NZ_CM000487.1.
DR AlphaFoldDB; P39766; -.
DR SMR; P39766; -.
DR STRING; 224308.BSU15480; -.
DR TCDB; 2.A.40.1.4; the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.
DR PRIDE; P39766; -.
DR EnsemblBacteria; CAB13422; CAB13422; BSU_15480.
DR GeneID; 64303439; -.
DR GeneID; 936604; -.
DR KEGG; bsu:BSU15480; -.
DR PATRIC; fig|224308.179.peg.1687; -.
DR eggNOG; COG2233; Bacteria.
DR InParanoid; P39766; -.
DR OMA; INHGIGM; -.
DR PhylomeDB; P39766; -.
DR BioCyc; BSUB:BSU15480-MON; -.
DR PRO; PR:P39766; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015205; F:nucleobase transmembrane transporter activity; IEA:UniProt.
DR InterPro; IPR006043; NCS2.
DR InterPro; IPR006042; Xan_ur_permease.
DR Pfam; PF00860; Xan_ur_permease; 1.
DR TIGRFAMs; TIGR00801; ncs2; 1.
DR PROSITE; PS01116; XANTH_URACIL_PERMASE; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..435
FT /note="Uracil permease"
FT /id="PRO_0000165957"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 231..260
FT /note="SPSVTLGIAAAMVPVAFVTMSEHIGHQMVL -> HRQLRSASQPQWFLSHLS
FT QCQSISATNGAE (in Ref. 1; AAA21266)"
FT /evidence="ECO:0000305"
FT CONFLICT 271..272
FT /note="KP -> A (in Ref. 1; AAA21266)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 435 AA; 45452 MW; 2EB735352A034D33 CRC64;
MSKKKVNLGV RDVPTPFSWV SFSLQHLFAM FGSTILVPKL VGMSPAVALV TSGIGTLAYL
LITKGQIPAY LGSSFAFISP IILVKATGGP GAAMVGAFLA GLVYGLIALL IRQLGTGWLM
KILPPVVVGP VIIVIGLGLA STAVNMAMYA DPNASELVYS LKHFSVAGVT LAITIICAIF
LRGFLSLIPV LIGIIGGYLF ALTQGIVNFQ PVLDAKWFAV PEFIIPFKDY SPSVTLGIAA
AMVPVAFVTM SEHIGHQMVL SKVVGQDFIK KPGLHRSIMG DSVATILASL IGGPPTTTYG
ENIGVLAITR VFSVFVIGGA AVIALCFGFI GKISALISSV PSAVMGGVSF LLFGIIASSG
LRMLIDNKID YENNRNLIIT SVILVIGVGG AFIQVSQGGF QVSGMALAAI VGVILNLILP
QAKEEQADTS EQHHI
