ID   PYRR_LACLA              Reviewed;         173 AA.
AC   Q9CF77;
DT   30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Pyrimidine operon regulatory protein {ECO:0000255|HAMAP-Rule:MF_01219};
GN   Name=pyrR {ECO:0000255|HAMAP-Rule:MF_01219}; OrderedLocusNames=LL1604;
GN   ORFNames=L0227;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- FUNCTION: Regulates transcriptional attenuation of the pyrimidine
CC       nucleotide (pyr) operon in response to exogenous pyrimidines, probably
CC       by binding to specific sites on pyr mRNA. This probably disrupts an
CC       antiterminator hairpin in the RNA and favors formation of a downstream
CC       transcription terminator, leading to a reduced expression of downstream
CC       genes (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the purine/pyrimidine phosphoribosyltransferase
CC       family. PyrR subfamily. {ECO:0000255|HAMAP-Rule:MF_01219}.
CC   -!- CAUTION: Unlike for B.subtilis, the PyrR protein of L.lactis was shown
CC       not to encode UPRTase activity. {ECO:0000305}.
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DR   EMBL; AE005176; AAK05702.1; -; Genomic_DNA.
DR   PIR; D86825; D86825.
DR   RefSeq; NP_267760.1; NC_002662.1.
DR   RefSeq; WP_010906054.1; NC_002662.1.
DR   AlphaFoldDB; Q9CF77; -.
DR   SMR; Q9CF77; -.
DR   STRING; 272623.L0227; -.
DR   PaxDb; Q9CF77; -.
DR   EnsemblBacteria; AAK05702; AAK05702; L0227.
DR   GeneID; 60356360; -.
DR   GeneID; 66442528; -.
DR   KEGG; lla:L0227; -.
DR   PATRIC; fig|272623.7.peg.1725; -.
DR   eggNOG; COG2065; Bacteria.
DR   HOGENOM; CLU_094234_2_1_9; -.
DR   OMA; PIQPDFC; -.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004845; F:uracil phosphoribosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-UniRule.
DR   CDD; cd06223; PRTases_typeI; 1.
DR   Gene3D; 3.40.50.2020; -; 1.
DR   HAMAP; MF_01219; PyrR; 1.
DR   InterPro; IPR000836; PRibTrfase_dom.
DR   InterPro; IPR029057; PRTase-like.
DR   InterPro; IPR023050; PyrR.
DR   Pfam; PF00156; Pribosyltran; 1.
DR   SUPFAM; SSF53271; SSF53271; 1.
PE   3: Inferred from homology;
KW   Reference proteome; RNA-binding; Transcription; Transcription regulation;
KW   Transcription termination.
FT   CHAIN           1..173
FT                   /note="Pyrimidine operon regulatory protein"
FT                   /id="PRO_0000183039"
FT   MOTIF           93..105
FT                   /note="PRPP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01219"
FT   BINDING         40..41
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         97..105
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         130
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   173 AA;  19831 MW;  608317C1E9E5876E CRC64;
     MARKEIIDEI TMKRAITRIT YEIIERNKEL DKLVLIGIKT RGVYLAKRIQ ERLQQLEGLE
     IPFGELDTRP FRDDKQAQED TTEIDIDITG KDVILVDDVL YTGRTIRAAI DGIVKLGRPA
     RVQLAVLVDR GHRELPIRAD YVGKNIPTGR DEEIIVQMSE HDGNDSILIK RED