ID   PYS1_PSEAI              Reviewed;         618 AA.
AC   Q06583;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Pyocin-S1;
DE            EC=3.1.-.-;
DE   AltName: Full=Killer protein;
GN   Name=pys1;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-13 AND
RP   484-504.
RC   STRAIN=NIH-H;
RX   PubMed=8491711; DOI=10.1128/jb.175.10.2907-2916.1993;
RA   Sano Y., Matsui H., Kobayashi M., Kageyama M.;
RT   "Molecular structures and functions of pyocins S1 and S2 in Pseudomonas
RT   aeruginosa.";
RL   J. Bacteriol. 175:2907-2916(1993).
CC   -!- FUNCTION: Causes breakdown of chromosomal DNA as well as complete
CC       inhibition of lipid synthesis in sensitive cells.
CC   -!- SUBUNIT: Purified pyocin S1 makes up a complex of the two (large and
CC       small) proteins. The large protein, but not the pyocin complex, shows
CC       in vitro DNase activity.
CC   -!- MISCELLANEOUS: Pyocins contain N-terminal receptor-binding domain,
CC       translocation domain and C-terminal DNase domain.
CC   -!- SIMILARITY: Belongs to the colicin/pyosin nuclease family.
CC       {ECO:0000305}.
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DR   EMBL; D12707; BAA02201.1; -; Genomic_DNA.
DR   PIR; A36907; A36907.
DR   AlphaFoldDB; Q06583; -.
DR   SMR; Q06583; -.
DR   eggNOG; COG4104; Bacteria.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0005102; F:signaling receptor binding; IEA:InterPro.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   CDD; cd00085; HNHc; 1.
DR   Gene3D; 3.90.540.10; -; 1.
DR   InterPro; IPR037146; Colicin/pyocin_DNase_dom_sf.
DR   InterPro; IPR044925; His-Me_finger_sf.
DR   InterPro; IPR003615; HNH_nuc.
DR   InterPro; IPR003060; Pyocin_killer.
DR   InterPro; IPR016128; Pyosin/cloacin_T_dom.
DR   InterPro; IPR036302; Pyosin/cloacin_T_dom_sf.
DR   Pfam; PF06958; Pyocin_S; 1.
DR   PRINTS; PR01300; PYOCINKILLER.
DR   SMART; SM00507; HNHc; 1.
DR   SUPFAM; SSF54060; SSF54060; 1.
DR   SUPFAM; SSF69369; SSF69369; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Endonuclease; Hydrolase; Nuclease.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:8491711"
FT   CHAIN           2..618
FT                   /note="Pyocin-S1"
FT                   /id="PRO_0000218685"
SQ   SEQUENCE   618 AA;  65629 MW;  EEBEF47F8163E24F CRC64;
     MARPIADLIH FNSTTVTASG DVYYGPGGGT GIGPIARPIE HGLDSSTENG WQEFESYADV
     GVDPRRYVPL QVKEKRREIE LQFRDAEKKL EASVQAELDK ADAALGPAKN LAPLDVINRS
     LTIVGNALQQ KNQKLLLNQK KITSLGAKNF LTRTAEEIGE QAVREGNING PEAYMRFLDR
     EMEGLTAAYN VKLFTEAISS LQIRMNTLTA AKASIEAAAA NKAREQAAAE AKRKAEEQAR
     QQAAIRAANT YAMPANGSVV ATAAGRGLIQ VAQGAASLAQ AISDAIAVLG RVLASAPSVM
     AVGFASLTYS SRTAEQWQDQ TPDSVRYALG MDAAKLGLPP SVNLNAVAKA SGTVDLPMRL
     TNEARGNTTT LSVVSTDGVS VPKAVPVRMA AYNATTGLYE VTVPSTTAEA PPLILTWTPA
     SPPGNQNPSS TTPVVPKPVP VYEGATLTPV KATPETYPGV ITLPEDLIIG FPADSGIKPI
     YVMFRDPRDV PGAATGKGQP VSGNWLGAAS QGEGAPIPSQ IADKLRGKTF KNWRDFREQF
     WIAVANDPEL SKQFNPGSLA VMRDGGAPYV RESEQAGGRI KIEIHHKVRV ADGGGVYNMG
     NLVAVTPKRH IEIHKGGK