PYX_DROME
ID PYX_DROME Reviewed; 956 AA.
AC Q9W0T5; Q8I0I4;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Transient receptor potential channel pyrexia;
GN Name=pyx; ORFNames=CG17142;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Testis;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=15731759; DOI=10.1038/ng1513;
RA Lee Y., Lee Y., Lee J., Bang S., Hyun S., Kang J., Hong S.-T., Bae E.,
RA Kaang B.-K., Kim J.;
RT "Pyrexia is a new thermal transient receptor potential channel endowing
RT tolerance to high temperatures in Drosophila melanogaster.";
RL Nat. Genet. 37:305-310(2005).
CC -!- FUNCTION: Receptor-activated non-selective cation channel involved in
CC protection or tolerance from high temperature stress. Activated by
CC temperatures above 40 degrees Celsius. More permeable to K(+) than to
CC Na(+). May act in stress protection allow flies to survive in natural
CC environments. {ECO:0000269|PubMed:15731759}.
CC -!- SUBUNIT: Homooligomer; between isoform A and isoform B. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=Q9W0T5-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9W0T5-2; Sequence=VSP_013426;
CC -!- TISSUE SPECIFICITY: Expressed in various peripheral nerves and the
CC central nerves in embryos. In adults, it is expressed in sensory
CC neurons lying beneath the bristles around eyes, neurons innervating the
CC bristles on the back of thorax and neurons in maxillary palps,
CC proboscis and antennae. Expressed in multidendritic neurons, which
CC mediate temperature sensing, as well as non-multidendritic neurons in
CC larval epidermis. Localizes ubiquitously throughout neurites.
CC {ECO:0000269|PubMed:15731759}.
CC -!- SIMILARITY: Belongs to the transient receptor (TC 1.A.4) family. STrpC
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAF47356.2; -; Genomic_DNA.
DR EMBL; AE014296; AAN11430.1; -; Genomic_DNA.
DR EMBL; BT001363; AAN71118.1; -; mRNA.
DR EMBL; BT010317; AAQ23635.1; -; mRNA.
DR RefSeq; NP_612015.1; NM_138171.2. [Q9W0T5-1]
DR RefSeq; NP_728501.1; NM_167813.2. [Q9W0T5-2]
DR AlphaFoldDB; Q9W0T5; -.
DR SMR; Q9W0T5; -.
DR BioGRID; 63600; 1.
DR IntAct; Q9W0T5; 2.
DR STRING; 7227.FBpp0072423; -.
DR TCDB; 1.A.4.6.4; the transient receptor potential ca(2+) channel (trp-cc) family.
DR GlyGen; Q9W0T5; 1 site.
DR PaxDb; Q9W0T5; -.
DR PRIDE; Q9W0T5; -.
DR DNASU; 38037; -.
DR EnsemblMetazoa; FBtr0072524; FBpp0072423; FBgn0035113. [Q9W0T5-1]
DR EnsemblMetazoa; FBtr0072525; FBpp0072424; FBgn0035113. [Q9W0T5-2]
DR GeneID; 38037; -.
DR KEGG; dme:Dmel_CG17142; -.
DR UCSC; CG17142-RA; d. melanogaster. [Q9W0T5-1]
DR CTD; 38037; -.
DR FlyBase; FBgn0035113; pyx.
DR VEuPathDB; VectorBase:FBgn0035113; -.
DR eggNOG; KOG0510; Eukaryota.
DR GeneTree; ENSGT00940000156118; -.
DR HOGENOM; CLU_009006_0_0_1; -.
DR InParanoid; Q9W0T5; -.
DR OMA; DVRSQFG; -.
DR PhylomeDB; Q9W0T5; -.
DR SignaLink; Q9W0T5; -.
DR BioGRID-ORCS; 38037; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38037; -.
DR PRO; PR:Q9W0T5; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035113; Expressed in mouthpart and 13 other tissues.
DR Genevisible; Q9W0T5; DM.
DR GO; GO:0034703; C:cation channel complex; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; IC:UniProtKB.
DR GO; GO:0005262; F:calcium channel activity; ISS:FlyBase.
DR GO; GO:0005261; F:cation channel activity; IDA:UniProtKB.
DR GO; GO:0006816; P:calcium ion transport; IDA:FlyBase.
DR GO; GO:0006812; P:cation transport; IDA:UniProtKB.
DR GO; GO:0048060; P:negative gravitaxis; IMP:FlyBase.
DR GO; GO:0006813; P:potassium ion transport; IDA:FlyBase.
DR GO; GO:0009408; P:response to heat; IMP:UniProtKB.
DR Gene3D; 1.25.40.20; -; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR005821; Ion_trans_dom.
DR Pfam; PF00023; Ank; 1.
DR Pfam; PF12796; Ank_2; 3.
DR Pfam; PF00520; Ion_trans; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 9.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 7.
PE 2: Evidence at transcript level;
KW Alternative splicing; ANK repeat; Glycoprotein; Ion channel; Ion transport;
KW Membrane; Reference proteome; Repeat; Sensory transduction; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..956
FT /note="Transient receptor potential channel pyrexia"
FT /id="PRO_0000215375"
FT TOPO_DOM 1..491
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 513..525
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 526..546
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 547..565
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 566..584
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 585..601
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 602..622
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 623..638
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 639..659
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 660..701
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 702..722
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 723..956
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 132..161
FT /note="ANK 1"
FT REPEAT 166..195
FT /note="ANK 2"
FT REPEAT 198..227
FT /note="ANK 3"
FT REPEAT 231..260
FT /note="ANK 4"
FT REPEAT 265..294
FT /note="ANK 5"
FT REPEAT 298..327
FT /note="ANK 6"
FT REPEAT 331..362
FT /note="ANK 7"
FT REPEAT 366..395
FT /note="ANK 8"
FT CARBOHYD 666
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..418
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_013426"
SQ SEQUENCE 956 AA; 107920 MW; 3C2AD5D0A8433FD8 CRC64;
MENVRFSIIE NDLKWNSESD PTADVDLLLD KRSISSEANS AGVFSDEAHE IFARQQQNQI
LWDLDEVKET LTESPGGKHV VDMVQSGCFL ELMTDSADCN LALICCSVFG SVENTLFLLK
HYNADPNVAD SRGRTPLHFA CCRANAPIAK VLLDFGADPN RWDARKEVTS LHCAASSKSV
ECILLLLRRK ASINIGIEKR SALHYAIDVN AVDCVEILLK YGADPNTPQV YTETPLHTAS
AAGFAKCVQL LLSHNADVRS QFGEGKVTAL HLAAENDYVE CARLLLEHRA EVDCRNASHQ
TPLHLACLSQ SIGTVDLLIS YGANVNAVYR DGRTALHAAI VKQSRSLDCC NALLKAGADV
NKADNYGYTP LHIAALNEFS SCVYTFIEHG ADITARTDGR VSALSFIVRR TPEIIPKLMQ
KLDSSIKAND QEIGDVDCQI KLDFRLLVPS SSMDRGETEL LLSLIEVGQK RILMHPLCET
FLFLKWRRIR KFFLMSLAYH TLFVILFTFY VIWVYVRCCK KEELCVAPGY VSTIGYLVII
LNLILLGKEV FQMAHGLRGY AKYWENWLQW TIGTGVLLCV TPETVRTDDL TAVPVWQHHV
AAIVILLVWL ELMMLVGRFP IFGVYVQMFT KVAVNFAKFL LAYICLLVAF GLSFAVLFND
YPAFENITWS FLKSITMMSG ELEFEDIFYG DYAVKFPVTA HIIFLSFVLL VTVILTNLMV
GLAVSDIQGL QVSATLDRLV RQAELVSRLE SLFFSRLLRS APTNLIQLCK RSALLRTSRD
KLQFTIRPND PRDNQLPEDI KLNVYKLVAE RRDRNQSLRR RQFENNYNIF SRSLQRQQQP
LHTDFLKPEP ATGTTKKTPQ NLFHMHELLR PRSATNVPQQ FRQEAEGTVQ MKNQANVLSA
VLAEVQAIKT QLVDLVAKFE RFSENATRKL NYSTDELCRL RQQGQSVASS HIRRHR
