QACA_STAAU
ID QACA_STAAU Reviewed; 514 AA.
AC P0A0J9; P23215;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Antiseptic resistance protein;
GN Name=qacA;
OS Staphylococcus aureus.
OG Plasmid VRSAp, and Plasmid pSK1.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=1280;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pSK1;
RX PubMed=2089219; DOI=10.1111/j.1365-2958.1990.tb00565.x;
RA Rouch D.A., Cram D.S., Diberardino D., Littlejohn T.G., Skurray R.A.;
RT "Efflux-mediated antiseptic resistance gene qacA from Staphylococcus
RT aureus: common ancestry with tetracycline- and sugar-transport proteins.";
RL Mol. Microbiol. 4:2051-2062(1990).
RN [2]
RP TOPOLOGY.
RX PubMed=8622987; DOI=10.1073/pnas.93.8.3630;
RA Paulsen I.T., Brown M.H., Littlejohn T.G., Mitchell B.A., Skurray R.A.;
RT "Multidrug resistance proteins QacA and QacB from Staphylococcus aureus:
RT membrane topology and identification of residues involved in substrate
RT specificity.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:3630-3635(1996).
CC -!- FUNCTION: Confers export-mediated resistance against antiseptic and
CC disinfectant compounds such as intercalating dyes, quaternary ammonium
CC salts and diamidines.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X56628; CAA39963.1; -; Genomic_DNA.
DR PIR; S12394; S12394.
DR RefSeq; WP_000622776.1; NZ_WKGQ01000034.1.
DR RefSeq; YP_003813123.1; NC_014369.1.
DR RefSeq; YP_536864.1; NC_007931.1.
DR AlphaFoldDB; P0A0J9; -.
DR SMR; P0A0J9; -.
DR TCDB; 2.A.1.3.4; the major facilitator superfamily (mfs).
DR GeneID; 66840931; -.
DR OMA; IAVTCKQ; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Cell membrane; Membrane; Plasmid; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..514
FT /note="Antiseptic resistance protein"
FT /id="PRO_0000173380"
FT TOPO_DOM 1..23
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 24..41
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 42..57
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 58..75
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 76..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 87..104
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 105..112
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 113..130
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 131..146
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 147..164
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 165..172
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 173..190
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 191..207
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 208..225
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 226..237
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 238..255
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 256..278
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 279..295
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 296..315
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 316..333
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 334..341
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 342..360
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 361..369
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 370..387
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 388..408
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 409..426
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 427..481
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:8622987"
FT TRANSMEM 482..499
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 500..514
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:8622987"
SQ SEQUENCE 514 AA; 55016 MW; 8FA5273C1E3D6E38 CRC64;
MISFFTKTTD MMTSKKRWTA LVVLAVSLFV VTMDMTILIM ALPELVRELE PSGTQQLWIV
DIYSLVLAGF IIPLSAFADK WGRKKALLTG FALFGLVSLA IFFAESAEFV IAIRFLLGIA
GALIMPTTLS MIRVIFENPK ERATALAVWS IASSIGAVFG PIIGGALLEQ FSWHSAFLIN
VPFAIIAVVA GLFLLPESKL SKEKSHSWDI PSTILSIAGM IGLVWSIKEF SKEGLADIIP
WVVIVLAITM IVIFVKRNLS SSDPMLDVRL FKKRSFSAGT IAAFMTMFAM ASVLLLASQW
LQVVEELSPF KAGLYLLPMA IGDMVFAPIA PGLAARFGPK IVLPSGIGIA AIGMFIMYFF
GHPLSYSTMA LALILVGAGM ASLAVASALI MLETPTSKAG NAAAVEESMY DLGNVFGVAV
LGSLSSMLYR VFLDISSFSS KGIVGDLAHV AEESVVGAVE VAKATGIKQL ANEAVTSFND
AFVATALVGG IIMIIISIVV YLLIPKSLDI TKQK
