QACC_STAAU
ID QACC_STAAU Reviewed; 107 AA.
AC P14319;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Quaternary ammonium compound-resistance protein QacC {ECO:0000305};
DE AltName: Full=Ethidium bromide resistance protein;
DE AltName: Full=Multidrug resistance protein;
DE AltName: Full=Quaternary ammonium determinant C;
GN Name=qacC {ECO:0000303|PubMed:1840534};
GN Synonyms=ebr {ECO:0000303|PubMed:1505735, ECO:0000303|PubMed:2602117},
GN smr {ECO:0000303|PubMed:1615062};
OS Staphylococcus aureus.
OG Plasmid pTZ20, Plasmid pWBG32, Plasmid pSK89, Plasmid pTZ22, and
OG Plasmid pKH8.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=1280;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=L20A; PLASMID=pTZ20;
RX PubMed=2602117; DOI=10.1093/nar/17.23.10103;
RA Sasatsu M., Shima K., Shibata Y., Kono M.;
RT "Nucleotide sequence of a gene that encodes resistance to ethidium bromide
RT from a transferable plasmid in Staphylococcus aureus.";
RL Nucleic Acids Res. 17:10103-10103(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC PLASMID=pWBG32;
RX PubMed=1615062; DOI=10.1016/0147-619x(92)90012-y;
RA Grinius L., Dreguniene G., Goldberg E.B., Liao C.H., Projan S.J.;
RT "A staphylococcal multidrug resistance gene product is a member of a new
RT protein family.";
RL Plasmid 27:119-129(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pSK89;
RX PubMed=1840534; DOI=10.1016/0378-1119(91)90224-y;
RA Littlejohn T.G., DiBerardino D., Messerotti L.J., Spiers S.J.,
RA Skurray R.A.;
RT "Structure and evolution of a family of genes encoding antiseptic and
RT disinfectant resistance in Staphylococcus aureus.";
RL Gene 101:59-66(1991).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=N20; PLASMID=pTZ22;
RX PubMed=1505735; DOI=10.1016/0378-1097(92)90514-o;
RA Sasatsu M., Shibata Y., Noguchi N., Kono M.;
RT "High-level resistance to ethidium bromide and antiseptics in
RT Staphylococcus aureus.";
RL FEMS Microbiol. Lett. 72:109-113(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SA2; PLASMID=pKH8;
RA Moon K.H., Im S.H., Yoon S.J., Kim W.K., Shin C.K., Lee D.W.;
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Leelaporn A., Firth N., Paulsen I.T., Hettiaratchi A., Skurray R.A.;
RL Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION.
RX PubMed=2778425; DOI=10.1099/00221287-135-1-1;
RA Tennent J.M., Lyon B.R., Midgley M., Jones I.G., Purewal A.S.,
RA Skurray R.A.;
RT "Physical and biochemical characterization of the qacA gene encoding
RT antiseptic and disinfectant resistance in Staphylococcus aureus.";
RL J. Gen. Microbiol. 135:1-10(1989).
RN [8]
RP FUNCTION, ACTIVITY REGULATION, SUBCELLULAR LOCATION, TOPOLOGY, AND
RP MUTAGENESIS OF PRO-31; CYS-42; TYR-59 AND TRP-62.
RX PubMed=7751293; DOI=10.1128/jb.177.10.2827-2833.1995;
RA Paulsen I.T., Brown M.H., Dunstan S.J., Skurray R.A.;
RT "Molecular characterization of the staphylococcal multidrug resistance
RT export protein QacC.";
RL J. Bacteriol. 177:2827-2833(1995).
CC -!- FUNCTION: Multidrug exporter. Is implicated for the resistance to
CC bacteriocidal quaternary ammonium compounds and ethidium bromide.
CC {ECO:0000269|PubMed:1615062, ECO:0000269|PubMed:7751293,
CC ECO:0000305|PubMed:2778425}.
CC -!- ACTIVITY REGULATION: Ethidium export is inhibited by N-ethylmaleimide
CC (NEM). {ECO:0000269|PubMed:7751293}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:7751293};
CC Multi-pass membrane protein {ECO:0000269|PubMed:7751293}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
CC {ECO:0000305}.
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DR EMBL; M37888; AAA26666.1; -; Genomic_DNA.
DR EMBL; X15574; CAA33598.1; -; Genomic_DNA.
DR EMBL; M33479; AAA26668.1; -; Genomic_DNA.
DR EMBL; M37889; AAB02114.1; -; Genomic_DNA.
DR EMBL; X62587; CAA44471.1; -; Genomic_DNA.
DR EMBL; X62587; CAA44472.1; -; Genomic_DNA.
DR EMBL; U50077; AAB07747.1; -; Genomic_DNA.
DR EMBL; U15783; AAA60379.1; -; Genomic_DNA.
DR PIR; JQ1439; JQ1439.
DR RefSeq; NP_863640.1; NC_005024.1.
DR RefSeq; NP_878005.1; NC_005054.1.
DR RefSeq; WP_001146389.1; NZ_WWCF01000015.1.
DR RefSeq; YP_002790936.1; NC_012547.1.
DR RefSeq; YP_006938404.1; NC_013339.1.
DR RefSeq; YP_006938512.1; NC_013342.1.
DR RefSeq; YP_006938611.1; NC_013344.1.
DR RefSeq; YP_006938730.1; NC_013350.1.
DR RefSeq; YP_534903.1; NC_007928.1.
DR AlphaFoldDB; P14319; -.
DR BMRB; P14319; -.
DR SMR; P14319; -.
DR TCDB; 2.A.7.1.1; the drug/metabolite transporter (dmt) superfamily.
DR GeneID; 66852236; -.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR InterPro; IPR045324; Small_multidrug_res.
DR PANTHER; PTHR30561; PTHR30561; 1.
DR Pfam; PF00893; Multi_Drug_Res; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Plasmid; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..107
FT /note="Quaternary ammonium compound-resistance protein
FT QacC"
FT /id="PRO_0000108083"
FT TOPO_DOM 1..2
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:7751293"
FT TRANSMEM 3..20
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 21..29
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:7751293"
FT TRANSMEM 30..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 48..56
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:7751293"
FT TRANSMEM 57..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..85
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:7751293"
FT TRANSMEM 86..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..107
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:7751293"
FT MUTAGEN 31
FT /note="P->A,G: Decrease in resistance to ethidium bromide,
FT but does not affect resistance to quaternary ammonium
FT compounds."
FT /evidence="ECO:0000269|PubMed:7751293"
FT MUTAGEN 42
FT /note="C->A,G: Decrease in resistance to ethidium bromide,
FT but does not affect resistance to quaternary ammonium
FT compounds."
FT /evidence="ECO:0000269|PubMed:7751293"
FT MUTAGEN 42
FT /note="C->D,E,I,K,L,P,R,V: Confers sensitivity to ethidium
FT bromide and quaternary ammonium compounds."
FT /evidence="ECO:0000269|PubMed:7751293"
FT MUTAGEN 42
FT /note="C->S: Confers sensitivity to ethidium bromide, but
FT does not affect resistance to quaternary ammonium
FT compounds."
FT /evidence="ECO:0000269|PubMed:7751293"
FT MUTAGEN 42
FT /note="C->T: No change in activity."
FT /evidence="ECO:0000269|PubMed:7751293"
FT MUTAGEN 59
FT /note="Y->S,W: Confers sensitivity to ethidium bromide and
FT quaternary ammonium compounds."
FT /evidence="ECO:0000269|PubMed:7751293"
FT MUTAGEN 62
FT /note="W->F,Y: Confers sensitivity to ethidium bromide and
FT quaternary ammonium compounds."
FT /evidence="ECO:0000269|PubMed:7751293"
SQ SEQUENCE 107 AA; 11673 MW; 9A0155B54A052E1F CRC64;
MPYIYLIIAI STEVIGSAFL KSSEGFSKFI PSLGTIISFG ICFYFLSKTM QHLPLNITYA
TWAGLGLVLT TVVSIIIFKE QINLITIVSI VLIIVGVVSL NIFGTSH
