QCR10_YEAST
ID QCR10_YEAST Reviewed; 77 AA.
AC P37299; D3DKR8; O13538; Q9T2W2;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Cytochrome b-c1 complex subunit 10, mitochondrial;
DE AltName: Full=Complex III subunit 10;
DE AltName: Full=Complex III subunit XI;
DE AltName: Full=Ubiquinol-cytochrome c oxidoreductase subunit 10;
DE AltName: Full=Ubiquinol-cytochrome c reductase 8.5 kDa protein;
GN Name=QCR10; OrderedLocusNames=YHR001W-A; ORFNames=YHR001BW;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8175712; DOI=10.1016/s0021-9258(18)99967-9;
RA Brandt U., Uribe S., Schaegger H., Trumpower B.L.;
RT "Isolation and characterization of QCR10, the nuclear gene encoding the
RT 8.5-kDa subunit 10 of the Saccharomyces cerevisiae cytochrome bc1
RT complex.";
RL J. Biol. Chem. 269:12947-12953(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP PROTEIN SEQUENCE OF 2-29 AND 38-59.
RC STRAIN=GM50-3C;
RX PubMed=1325905; DOI=10.1111/j.1432-1033.1992.tb17197.x;
RA Geier B.M., Schaegger H., Brandt U., Colson A.-M., von Jagow G.;
RT "Point mutation in cytochrome b of yeast ubihydroquinone:cytochrome-c
RT oxidoreductase causing myxothiazol resistance and facilitated dissociation
RT of the iron-sulfur subunit.";
RL Eur. J. Biochem. 208:375-380(1992).
RN [5]
RP FORMATION OF CYTOCHROME BC1-CYTOCHROME C OXIDASE SUPERCOMPLEX.
RX PubMed=10775262; DOI=10.1093/emboj/19.8.1777;
RA Schaegger H., Pfeiffer K.;
RT "Supercomplexes in the respiratory chains of yeast and mammalian
RT mitochondria.";
RL EMBO J. 19:1777-1783(2000).
RN [6]
RP FORMATION OF CYTOCHROME BC1-CYTOCHROME C OXIDASE SUPERCOMPLEX.
RX PubMed=10764779; DOI=10.1074/jbc.m001901200;
RA Cruciat C.M., Brunner S., Baumann F., Neupert W., Stuart R.A.;
RT "The cytochrome bc1 and cytochrome c oxidase complexes associate to form a
RT single supracomplex in yeast mitochondria.";
RL J. Biol. Chem. 275:18093-18098(2000).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.23 ANGSTROMS).
RX PubMed=30598556; DOI=10.1038/s41594-018-0169-7;
RA Rathore S., Berndtsson J., Marin-Buera L., Conrad J., Carroni M.,
RA Brzezinski P., Ott M.;
RT "Cryo-EM structure of the yeast respiratory supercomplex.";
RL Nat. Struct. Mol. Biol. 26:50-57(2019).
RN [9]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.35 ANGSTROMS).
RX PubMed=30598554; DOI=10.1038/s41594-018-0172-z;
RA Hartley A.M., Lukoyanova N., Zhang Y., Cabrera-Orefice A., Arnold S.,
RA Meunier B., Pinotsis N., Marechal A.;
RT "Structure of yeast cytochrome c oxidase in a supercomplex with cytochrome
RT bc1.";
RL Nat. Struct. Mol. Biol. 26:78-83(2019).
CC -!- FUNCTION: Component of the ubiquinol-cytochrome c oxidoreductase, a
CC multisubunit transmembrane complex that is part of the mitochondrial
CC electron transport chain which drives oxidative phosphorylation. The
CC respiratory chain contains 3 multisubunit complexes succinate
CC dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase
CC (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase
CC (complex IV, CIV), that cooperate to transfer electrons derived from
CC NADH and succinate to molecular oxygen, creating an electrochemical
CC gradient over the inner membrane that drives transmembrane transport
CC and the ATP synthase. The cytochrome b-c1 complex catalyzes electron
CC transfer from ubiquinol to cytochrome c, linking this redox reaction to
CC translocation of protons across the mitochondrial inner membrane, with
CC protons being carried across the membrane as hydrogens on the quinol.
CC In the process called Q cycle, 2 protons are consumed from the matrix,
CC 4 protons are released into the intermembrane space and 2 electrons are
CC passed to cytochrome c (Probable). QCR10 has a role in CIII assembly
CC and RIP1 stability (PubMed:8175712, PubMed:30598556, PubMed:30598554).
CC {ECO:0000269|PubMed:30598554, ECO:0000269|PubMed:30598556,
CC ECO:0000269|PubMed:8175712, ECO:0000305|PubMed:30598554}.
CC -!- SUBUNIT: Component of the ubiquinol-cytochrome c oxidoreductase
CC (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme
CC composed of 10 subunits. The complex is composed of 3 respiratory
CC subunits cytochrome b (COB), cytochrome c1 (CYT1) and Rieske protein
CC (RIP1), 2 core protein subunits COR1 and QCR2, and 5 low-molecular
CC weight protein subunits QCR6, QCR7, QCR8, QCR9 and QCR10
CC (PubMed:30598554). The complex exists as an obligatory dimer and forms
CC supercomplexes (SCs) in the inner mitochondrial membrane with a monomer
CC or a dimer of cytochrome c oxidase (complex IV, CIV), resulting in 2
CC different assemblies (supercomplexes III(2)IV and III(2)IV(2))
CC (PubMed:10775262, PubMed:10764779, PubMed:30598556, PubMed:30598554).
CC QCR10 interacts with CYT1, QCR9 and RIP1 on the intermembrane space
CC (IMS) and with COR2 and QCR7 on the matrix side (PubMed:30598556,
CC PubMed:30598554). {ECO:0000269|PubMed:10764779,
CC ECO:0000269|PubMed:10775262, ECO:0000269|PubMed:30598554,
CC ECO:0000269|PubMed:30598556}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:30598554}; Single-pass membrane protein
CC {ECO:0000269|PubMed:30598554}.
CC -!- MISCELLANEOUS: Present with 5590 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the UQCR11/QCR10 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB68434.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U07275; AAA19322.1; -; Unassigned_DNA.
DR EMBL; U10555; AAB68434.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006934; DAA06688.1; -; Genomic_DNA.
DR PIR; A53768; A53768.
DR RefSeq; NP_011864.1; NM_001181426.1.
DR PDB; 6GIQ; EM; 3.23 A; U/V=1-77.
DR PDB; 6HU9; EM; 3.35 A; J/U=1-77.
DR PDB; 6T0B; EM; 2.80 A; J/U=1-77.
DR PDB; 6T15; EM; 3.29 A; J/U=1-77.
DR PDBsum; 6GIQ; -.
DR PDBsum; 6HU9; -.
DR PDBsum; 6T0B; -.
DR PDBsum; 6T15; -.
DR AlphaFoldDB; P37299; -.
DR SMR; P37299; -.
DR BioGRID; 36426; 81.
DR ComplexPortal; CPX-567; Mitochondrial respiratory chain complex III.
DR DIP; DIP-7141N; -.
DR IntAct; P37299; 3.
DR STRING; 4932.YHR001W-A; -.
DR MaxQB; P37299; -.
DR PaxDb; P37299; -.
DR PRIDE; P37299; -.
DR TopDownProteomics; P37299; -.
DR EnsemblFungi; YHR001W-A_mRNA; YHR001W-A; YHR001W-A.
DR GeneID; 856390; -.
DR KEGG; sce:YHR001W-A; -.
DR SGD; S000003529; QCR10.
DR VEuPathDB; FungiDB:YHR001W-A; -.
DR eggNOG; ENOG502S83P; Eukaryota.
DR HOGENOM; CLU_152072_2_0_1; -.
DR InParanoid; P37299; -.
DR OMA; VFTEGWP; -.
DR BioCyc; MetaCyc:G3O-31243-MON; -.
DR BioCyc; YEAST:G3O-31243-MON; -.
DR PRO; PR:P37299; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P37299; protein.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:ComplexPortal.
DR GO; GO:0005750; C:mitochondrial respiratory chain complex III; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0009060; P:aerobic respiration; IGI:SGD.
DR GO; GO:0045333; P:cellular respiration; IDA:ComplexPortal.
DR GO; GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IDA:ComplexPortal.
DR GO; GO:1902600; P:proton transmembrane transport; IEA:GOC.
DR InterPro; IPR019182; Cytochrome_b-c1_su10_fun.
DR PANTHER; PTHR28254; PTHR28254; 1.
DR Pfam; PF09796; QCR10; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transmembrane; Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:1325905"
FT CHAIN 2..77
FT /note="Cytochrome b-c1 complex subunit 10, mitochondrial"
FT /id="PRO_0000193563"
FT TOPO_DOM 2..21
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000269|PubMed:30598554"
FT TRANSMEM 22..45
FT /note="Helical"
FT /evidence="ECO:0000269|PubMed:30598554"
FT TOPO_DOM 46..77
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000269|PubMed:30598554"
FT STRAND 9..11
FT /evidence="ECO:0007829|PDB:6T15"
FT STRAND 14..17
FT /evidence="ECO:0007829|PDB:6T0B"
FT HELIX 20..44
FT /evidence="ECO:0007829|PDB:6T0B"
FT TURN 45..47
FT /evidence="ECO:0007829|PDB:6T0B"
FT HELIX 49..54
FT /evidence="ECO:0007829|PDB:6T0B"
FT TURN 55..57
FT /evidence="ECO:0007829|PDB:6T0B"
FT STRAND 60..63
FT /evidence="ECO:0007829|PDB:6T0B"
SQ SEQUENCE 77 AA; 8593 MW; B9212B4FD194BB3E CRC64;
MAYTSHLSSK TGLHFGRLSL RSLTAYAPNL MLWGGASMLG LFVFTEGWPK FQDTLYKKIP
LLGPTLEDHT PPEDKPN
