QCR9_MOUSE
ID QCR9_MOUSE Reviewed; 64 AA.
AC Q8R1I1;
DT 31-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Cytochrome b-c1 complex subunit 9;
DE AltName: Full=Complex III subunit 9;
DE AltName: Full=Complex III subunit X;
DE AltName: Full=Cytochrome c1 non-heme 7 kDa protein;
DE AltName: Full=Ubiquinol-cytochrome c reductase complex 7.2 kDa protein;
GN Name=Uqcr10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PROTEIN SEQUENCE OF 10-16 AND 35-51, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [3]
RP SUBUNIT.
RX PubMed=19026783; DOI=10.1016/j.molcel.2008.10.021;
RA Acin-Perez R., Fernandez-Silva P., Peleato M.L., Perez-Martos A.,
RA Enriquez J.A.;
RT "Respiratory active mitochondrial supercomplexes.";
RL Mol. Cell 32:529-539(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP INTERACTION WITH STMP1.
RX PubMed=35101990; DOI=10.1073/pnas.2120476119;
RA Makarewich C.A., Munir A.Z., Bezprozvannaya S., Gibson A.M., Young Kim S.,
RA Martin-Sandoval M.S., Mathews T.P., Szweda L.I., Bassel-Duby R.,
RA Olson E.N.;
RT "The cardiac-enriched microprotein mitolamban regulates mitochondrial
RT respiratory complex assembly and function in mice.";
RL Proc. Natl. Acad. Sci. U.S.A. 119:0-0(2022).
CC -!- FUNCTION: Component of the ubiquinol-cytochrome c oxidoreductase, a
CC multisubunit transmembrane complex that is part of the mitochondrial
CC electron transport chain which drives oxidative phosphorylation. The
CC respiratory chain contains 3 multisubunit complexes succinate
CC dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase
CC (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase
CC (complex IV, CIV), that cooperate to transfer electrons derived from
CC NADH and succinate to molecular oxygen, creating an electrochemical
CC gradient over the inner membrane that drives transmembrane transport
CC and the ATP synthase. The cytochrome b-c1 complex catalyzes electron
CC transfer from ubiquinol to cytochrome c, linking this redox reaction to
CC translocation of protons across the mitochondrial inner membrane, with
CC protons being carried across the membrane as hydrogens on the quinol.
CC In the process called Q cycle, 2 protons are consumed from the matrix,
CC 4 protons are released into the intermembrane space and 2 electrons are
CC passed to cytochrome c. {ECO:0000250|UniProtKB:P22289}.
CC -!- SUBUNIT: Component of the ubiquinol-cytochrome c oxidoreductase
CC (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme
CC composed of 11 subunits. The complex is composed of 3 respiratory
CC subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core
CC protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular
CC weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8,
CC UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske
CC protein UQCRFS1 (By similarity). The complex exists as an obligatory
CC dimer and forms supercomplexes (SCs) in the inner mitochondrial
CC membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and
CC cytochrome c oxidase (complex IV, CIV), resulting in different
CC assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex
CC MCI(2)III(2)IV(2)) (PubMed:19026783). Interacts with STMP1
CC (PubMed:35101990). {ECO:0000250|UniProtKB:P00130,
CC ECO:0000269|PubMed:19026783, ECO:0000269|PubMed:35101990}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:P22289}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:P22289}.
CC -!- SIMILARITY: Belongs to the UQCR10/QCR9 family. {ECO:0000305}.
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DR EMBL; BC024518; AAH24518.1; -; mRNA.
DR CCDS; CCDS36098.1; -.
DR RefSeq; NP_932096.1; NM_197979.2.
DR PDB; 7O37; EM; 3.20 A; J/U=2-64.
DR PDB; 7O3C; EM; 3.30 A; J/U=2-64.
DR PDB; 7O3E; EM; 3.60 A; J/U=2-64.
DR PDB; 7O3H; EM; 2.60 A; J/U=2-64.
DR PDBsum; 7O37; -.
DR PDBsum; 7O3C; -.
DR PDBsum; 7O3E; -.
DR PDBsum; 7O3H; -.
DR AlphaFoldDB; Q8R1I1; -.
DR SMR; Q8R1I1; -.
DR BioGRID; 211253; 42.
DR ComplexPortal; CPX-563; Mitochondrial respiratory chain complex III.
DR CORUM; Q8R1I1; -.
DR IntAct; Q8R1I1; 3.
DR MINT; Q8R1I1; -.
DR STRING; 10090.ENSMUSP00000054856; -.
DR iPTMnet; Q8R1I1; -.
DR PhosphoSitePlus; Q8R1I1; -.
DR EPD; Q8R1I1; -.
DR jPOST; Q8R1I1; -.
DR MaxQB; Q8R1I1; -.
DR PaxDb; Q8R1I1; -.
DR PRIDE; Q8R1I1; -.
DR ProteomicsDB; 301841; -.
DR Antibodypedia; 24577; 91 antibodies from 22 providers.
DR DNASU; 66152; -.
DR Ensembl; ENSMUST00000058407; ENSMUSP00000054856; ENSMUSG00000059534.
DR GeneID; 66152; -.
DR KEGG; mmu:66152; -.
DR UCSC; uc007hvb.1; mouse.
DR CTD; 29796; -.
DR MGI; MGI:1913402; Uqcr10.
DR VEuPathDB; HostDB:ENSMUSG00000059534; -.
DR eggNOG; KOG3494; Eukaryota.
DR GeneTree; ENSGT00390000014052; -.
DR HOGENOM; CLU_171977_2_0_1; -.
DR InParanoid; Q8R1I1; -.
DR OMA; DIKHRYI; -.
DR OrthoDB; 1624769at2759; -.
DR PhylomeDB; Q8R1I1; -.
DR TreeFam; TF324385; -.
DR Reactome; R-MMU-611105; Respiratory electron transport.
DR BioGRID-ORCS; 66152; 23 hits in 73 CRISPR screens.
DR ChiTaRS; Uqcr10; mouse.
DR PRO; PR:Q8R1I1; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8R1I1; protein.
DR Bgee; ENSMUSG00000059534; Expressed in right kidney and 233 other tissues.
DR ExpressionAtlas; Q8R1I1; baseline and differential.
DR Genevisible; Q8R1I1; MM.
DR GO; GO:0005743; C:mitochondrial inner membrane; HDA:MGI.
DR GO; GO:0005750; C:mitochondrial respiratory chain complex III; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0045333; P:cellular respiration; IC:ComplexPortal.
DR GO; GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IBA:GO_Central.
DR Gene3D; 1.20.5.260; -; 1.
DR InterPro; IPR008027; QCR9.
DR InterPro; IPR036656; QCR9_sf.
DR PANTHER; PTHR12980; PTHR12980; 1.
DR Pfam; PF05365; UCR_UQCRX_QCR9; 1.
DR SUPFAM; SSF81514; SSF81514; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Respiratory chain; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..64
FT /note="Cytochrome b-c1 complex subunit 9"
FT /id="PRO_0000193554"
FT TOPO_DOM 2..21
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000250|UniProtKB:P00130"
FT TRANSMEM 22..47
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P00130"
FT TOPO_DOM 48..63
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250|UniProtKB:P00130"
FT HELIX 6..13
FT /evidence="ECO:0007829|PDB:7O3H"
FT HELIX 18..47
FT /evidence="ECO:0007829|PDB:7O3H"
FT TURN 49..51
FT /evidence="ECO:0007829|PDB:7O3H"
FT HELIX 53..56
FT /evidence="ECO:0007829|PDB:7O3H"
FT HELIX 57..59
FT /evidence="ECO:0007829|PDB:7O3H"
SQ SEQUENCE 64 AA; 7446 MW; 54DECD7452434CAC CRC64;
MSSPTIPSRL YSLLFRRTST FALTIAVGAL FFERAFDQGA DAIYEHINEG KLWKHIKHKY
ENKE
