QKI_CHICK
ID QKI_CHICK Reviewed; 340 AA.
AC Q9YH18; Q9PST2; Q9YH19; Q9YH20;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2006, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Protein quaking;
GN Name=QKI;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 3 AND 4).
RC STRAIN=Hubbard White Mountain; TISSUE=Testis;
RX PubMed=9547512;
RX DOI=10.1002/(sici)1098-2795(199805)50:1<70::aid-mrd9>3.0.co;2-u;
RA Mezquita J., Pau M., Mezquita C.;
RT "Four isoforms of the signal-transduction and RNA-binding protein QKI
RT expressed during chicken spermatogenesis.";
RL Mol. Reprod. Dev. 50:70-78(1998).
CC -!- FUNCTION: RNA-binding protein that plays a central role in
CC myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core
CC sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA
CC stability and protein translation. Required to protect and promote
CC stability of mRNAs which promotes oligodendrocyte differentiation.
CC Participates in mRNA transport by regulating the nuclear export of MBP
CC mRNA. Also involved in regulation of mRNA splicing of some pre-mRNA.
CC Acts as a translational repressor (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Does not require RNA to homodimerize (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1; Synonyms=QkI-1;
CC IsoId=Q9YH18-1; Sequence=Displayed;
CC Name=2; Synonyms=QkI-2;
CC IsoId=Q9YH18-2; Sequence=VSP_019203;
CC Name=3; Synonyms=QkI-3;
CC IsoId=Q9YH18-3; Sequence=VSP_019200, VSP_019203;
CC Name=4; Synonyms=QkI-4;
CC IsoId=Q9YH18-4; Sequence=VSP_019201, VSP_019202;
CC -!- MISCELLANEOUS: [Isoform 1]: Major isoform.
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DR EMBL; U86764; AAD00621.1; -; mRNA.
DR EMBL; U86765; AAD00622.1; -; Genomic_DNA.
DR EMBL; U86766; AAD00623.1; -; mRNA.
DR EMBL; U86767; AAD00624.1; -; mRNA.
DR RefSeq; NP_989641.2; NM_204310.2. [Q9YH18-1]
DR RefSeq; XP_015139487.1; XM_015284001.1. [Q9YH18-3]
DR RefSeq; XP_015139489.1; XM_015284003.1. [Q9YH18-2]
DR AlphaFoldDB; Q9YH18; -.
DR SMR; Q9YH18; -.
DR STRING; 9031.ENSGALP00000018821; -.
DR PaxDb; Q9YH18; -.
DR Ensembl; ENSGALT00000065122; ENSGALP00000057308; ENSGALG00000011555. [Q9YH18-1]
DR GeneID; 374204; -.
DR KEGG; gga:374204; -.
DR CTD; 9444; -.
DR VEuPathDB; HostDB:geneid_374204; -.
DR eggNOG; KOG1588; Eukaryota.
DR GeneTree; ENSGT00940000155310; -.
DR InParanoid; Q9YH18; -.
DR OrthoDB; 1565749at2759; -.
DR PhylomeDB; Q9YH18; -.
DR PRO; PR:Q9YH18; -.
DR Proteomes; UP000000539; Chromosome 3.
DR Bgee; ENSGALG00000011555; Expressed in cerebellum and 13 other tissues.
DR ExpressionAtlas; Q9YH18; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0045202; C:synapse; IEA:Ensembl.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0017124; F:SH3 domain binding; IEA:UniProtKB-KW.
DR GO; GO:0042759; P:long-chain fatty acid biosynthetic process; IEA:Ensembl.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0042552; P:myelination; IEA:Ensembl.
DR GO; GO:0010628; P:positive regulation of gene expression; IEA:Ensembl.
DR GO; GO:0048024; P:regulation of mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR GO; GO:0007286; P:spermatid development; IEA:Ensembl.
DR GO; GO:0035886; P:vascular associated smooth muscle cell differentiation; IEA:Ensembl.
DR GO; GO:0001570; P:vasculogenesis; IEA:Ensembl.
DR Gene3D; 3.30.1370.10; -; 1.
DR InterPro; IPR045071; BBP-like.
DR InterPro; IPR004087; KH_dom.
DR InterPro; IPR004088; KH_dom_type_1.
DR InterPro; IPR036612; KH_dom_type_1_sf.
DR InterPro; IPR032367; Quaking_NLS.
DR InterPro; IPR032377; STAR_dimer.
DR PANTHER; PTHR11208; PTHR11208; 1.
DR Pfam; PF00013; KH_1; 1.
DR Pfam; PF16551; Quaking_NLS; 1.
DR Pfam; PF16544; STAR_dimer; 1.
DR SMART; SM00322; KH; 1.
DR SUPFAM; SSF54791; SSF54791; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW mRNA processing; mRNA splicing; mRNA transport; Nucleus;
KW Reference proteome; RNA-binding; SH3-binding; Translation regulation;
KW Transport.
FT CHAIN 1..340
FT /note="Protein quaking"
FT /id="PRO_0000239377"
FT DOMAIN 87..153
FT /note="KH"
FT MOTIF 275..278
FT /note="SH3-binding"
FT VAR_SEQ 134
FT /note="K -> KVSFKSRDNHDPAVLEVE (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:9547512"
FT /id="VSP_019200"
FT VAR_SEQ 184..186
FT /note="EGE -> WET (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:9547512"
FT /id="VSP_019201"
FT VAR_SEQ 187..340
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:9547512"
FT /id="VSP_019202"
FT VAR_SEQ 213..220
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:9547512"
FT /id="VSP_019203"
FT CONFLICT 144
FT /note="W -> R (in Ref. 1; AAD00624)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="D -> G (in Ref. 1; AAD00623)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 340 AA; 37570 MW; B3129274D1BB0D45 CRC64;
MVGEMEAKEK PKPSPDYLMQ LMNDKKLMSS LPNFCGIFNH LERLLDEEIS RVRKDMYNDT
LNGSTEKRSA ELPDAVGPIV QLQEKLYVPV KEYPDFNFVG RILGPRGLTA KQLEAETGCK
IMVRGKGSMR DKKKEEQNRG KPNWEHLNED LHVLITVEDA QNRAEIKLKR AVEEVKKLLI
PAAEGEDSLK KMQLMELAIL NGTYRDANIK SPALAFSLAA TAQAPRIITG PAPVLPPAAL
RTPTPAGPTI MPLIRQIQTA VMPNGTPHPT AAIVPPGPEA GLIYTPYEYP YTLAPATSIL
EYPIEPSGVL GAVATKVRRH DMRVHPYQRI VTADRAATGN
