ID   QNRB4_ECOLX             Reviewed;         215 AA.
AC   Q2PT27;
DT   14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 2.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Pentapeptide repeat protein QnrB4 {ECO:0000303|PubMed:21781207};
DE   AltName: Full=Plasmid-mediated quinolone resistance determinant {ECO:0000303|PubMed:21781207};
GN   Name=qnrB4 {ECO:0000303|PubMed:16870791};
OS   Escherichia coli.
OG   Plasmid pDEC216, and Plasmid pEC37Tc.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX   PubMed=16870791; DOI=10.1128/aac.01647-05;
RA   Robicsek A., Strahilevitz J., Sahm D.F., Jacoby G.A., Hooper D.C.;
RT   "qnr prevalence in ceftazidime-resistant Enterobacteriaceae isolates from
RT   the United States.";
RL   Antimicrob. Agents Chemother. 50:2872-2874(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=37Tc; PLASMID=pEC37Tc;
RX   PubMed=21781207; DOI=10.1111/j.1469-0691.2011.03539.x;
RA   Mata C., Miro E., Toleman M., Rivera M.A., Walsh T.R., Navarro F.;
RT   "Association of bla(DHA-1) and qnrB genes carried by broad-host-range
RT   plasmids among isolates of Enterobacteriaceae at a Spanish hospital.";
RL   Clin. Microbiol. Infect. 17:1514-1517(2011).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DEC216; PLASMID=pDEC216;
RA   Hu G., Han Z.;
RT   "Genetic environment of DHA-1 in Escherichia coli.";
RL   Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION.
RX   PubMed=19060136; DOI=10.1128/jb.01205-08;
RA   Merens A., Matrat S., Aubry A., Lascols C., Jarlier V., Soussy C.J.,
RA   Cavallo J.D., Cambau E.;
RT   "The pentapeptide repeat proteins MfpAMt and QnrB4 exhibit opposite effects
RT   on DNA gyrase catalytic reactions and on the ternary gyrase-DNA-quinolone
RT   complex.";
RL   J. Bacteriol. 191:1587-1594(2009).
CC   -!- FUNCTION: Probably plays a role in resistance to quinolone antibiotics
CC       (PubMed:16870791). Only inhibits ATP-dependent DNA supercoiling by
CC       E.coli gyrase at high concentration (30 uM) (PubMed:19060136). Protects
CC       E.coli gyrase supercoiling activity from inhibition by fluoroquinolones
CC       (ciprofloxacin) at 0.1 uM, does not protect M.tuberculosis gyrase
CC       activity (PubMed:19060136). {ECO:0000269|PubMed:19060136,
CC       ECO:0000305|PubMed:16870791}.
CC   -!- MISCELLANEOUS: The gene for this protein has been isolated from
CC       plasmids in multiple Enterobacteriaceae (PubMed:16870791,
CC       PubMed:19060136). For experiments in situ it was cloned from E.cloacae
CC       strain HM05-184 but tested in E.coli (PubMed:19060136).
CC       {ECO:0000305|PubMed:16870791, ECO:0000305|PubMed:19060136}.
CC   -!- SIMILARITY: Belongs to the pentapeptide repeat protein family.
CC       {ECO:0000305}.
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DR   EMBL; DQ303921; ABC17630.2; -; Genomic_DNA.
DR   EMBL; HQ700359; AEJ36325.1; -; Genomic_DNA.
DR   EMBL; KC848778; AGI55898.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q2PT27; -.
DR   SMR; Q2PT27; -.
DR   PRIDE; Q2PT27; -.
DR   KEGG; ag:ABC17630; -.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR001646; 5peptide_repeat.
DR   Pfam; PF13599; Pentapeptide_4; 2.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Plasmid.
FT   CHAIN           1..215
FT                   /note="Pentapeptide repeat protein QnrB4"
FT                   /id="PRO_0000434156"
FT   DOMAIN          113..147
FT                   /note="Pentapeptide repeat"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   215 AA;  23979 MW;  B7E0163A0D04217A CRC64;
     MMTLALVGEK IDRNRFTGEK VENSTFFNCD FSGADLSGTE FIGCQFYDRE SQKGCNFSRA
     NLKDAIFKSC DLSMADFRNI NALGIEIRHC RAQGSDFRGA SFMNMITTRT WFCSAYITNT
     NLSYANFSKV VLEKCELWEN RWMGTQVLGA TFSGSDLSGG EFSSFDWRAA NVTHCDLTNS
     ELGDLDIRGV DLQGVKLDSY QASLLLERLG IAVMG