ID   QNR_VIBPA               Reviewed;         216 AA.
AC   Q87K03;
DT   14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Pentapeptide repeat protein VPA0095 {ECO:0000305};
GN   OrderedLocusNames=VPA0095 {ECO:0000312|EMBL:BAC61438.1};
OS   Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=223926;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RIMD 2210633;
RX   PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA   Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA   Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA   Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT   "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT   distinct from that of V. cholerae.";
RL   Lancet 361:743-749(2003).
RN   [2]
RP   FUNCTION, AND MUTAGENESIS OF CYS-115.
RC   STRAIN=8611;
RX   PubMed=15855551; DOI=10.1128/aac.49.5.2144-2145.2005;
RA   Saga T., Kaku M., Onodera Y., Yamachika S., Sato K., Takase H.;
RT   "Vibrio parahaemolyticus chromosomal qnr homologue VPA0095: demonstration
RT   by transformation with a mutated gene of its potential to reduce quinolone
RT   susceptibility in Escherichia coli.";
RL   Antimicrob. Agents Chemother. 49:2144-2145(2005).
CC   -!- FUNCTION: Has no effect when overexpressed in E.coli. When Cys-115 is
CC       mutated to Tyr and overexpressed it increases (fluoro)quinolone
CC       resistance in E.coli up to 16-fold for ciprofloxacin, levofloxacin and
CC       nalidixic acid. {ECO:0000269|PubMed:15855551}.
CC   -!- SIMILARITY: Belongs to the pentapeptide repeat protein family.
CC       {ECO:0000305}.
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DR   EMBL; BA000032; BAC61438.1; -; Genomic_DNA.
DR   RefSeq; NP_799605.1; NC_004605.1.
DR   RefSeq; WP_005482928.1; NC_004605.1.
DR   AlphaFoldDB; Q87K03; -.
DR   SMR; Q87K03; -.
DR   STRING; 223926.28808233; -.
DR   EnsemblBacteria; BAC61438; BAC61438; BAC61438.
DR   GeneID; 1190774; -.
DR   KEGG; vpa:VPA0095; -.
DR   PATRIC; fig|223926.6.peg.3055; -.
DR   eggNOG; COG1357; Bacteria.
DR   HOGENOM; CLU_033401_8_0_6; -.
DR   OMA; TGCNLSY; -.
DR   Proteomes; UP000002493; Chromosome 2.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR001646; 5peptide_repeat.
DR   Pfam; PF00805; Pentapeptide; 1.
DR   Pfam; PF13599; Pentapeptide_4; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Reference proteome.
FT   CHAIN           1..216
FT                   /note="Pentapeptide repeat protein VPA0095"
FT                   /id="PRO_0000434157"
FT   MUTAGEN         115
FT                   /note="C->Y: Increased resistance to (fluoro)quinolone
FT                   antibiotics when expressed in E.coli."
FT                   /evidence="ECO:0000269|PubMed:15855551"
SQ   SEQUENCE   216 AA;  24688 MW;  1012E2082D05297C CRC64;
     MLKTDLIFER ENFSHHDFQN ATFKNCHFYM CSFDHADLRD AKFIDCRFIE SKALEGCSFR
     FANLKDASFT NCMLAMSLFN GANCMGLELR KCDLKGANFQ GANFANRVSN TMFFCSAFIT
     GCNLTYCNFE RVLLEKCDLF ENRWNGANLA GATLKGSDLS RCEFSPEQWG TFNVEQCDLT
     HVELDGLDIR RVSLFGVKIC DWQQEQLLAP FGLIIL